Enzymatic/biochemical analysis of Actinomyces with commercial test kits with an emphasis on newly described species

Kerttula, Anne-Marie; Carlson, Petteri; Sarkonen, Nanna; Hall, Val; Könönen, Eija

doi:10.1016/j.anaerobe.2004.11.002

Cited by 6 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A. israelii has also been isolated from cases of postoperative keratitis (171) and primary infections of the lacrimal duct, i.e., canaliculitis (172)(173)(174). In addition, A. meyeri and A. georgiae have been found in specimens from cases of canaliculitis (172,175), while A. naeslundii was identified in a swab specimen taken from a cornea after trauma leading to keratitis (107). Of 59 eye secretions/tear fluid examined in a reference laboratory in Germany, 22% were positive for "A. viscosus," 19% were positive for A. israelii, 14% were positive for A. naeslundii, and 10% were positive for A. gerencseriae and A. odontolyticus each; A. israelii and A. gerencseriae were isolated from canaliculitis cases, whereas "A. viscosus," A. naeslundii, and A. odontolyticus were more common in cases of conjunctivitis (13).…”

Section: Eye Infectionsmentioning

confidence: 99%

“…Nipple piercing was suggested to be a predisposing factor for Actinomyces infection of the breast in two patients, one due to A. radingae (204) and another due to A. turicensis together with a Gram-positive anaerobic coccus, Peptoniphilus harei (206). Other sites of the upper body with abscesses with an involvement of Actinomyces organisms include the face, neck, axilla, armpit, chest, and/or back, with A. europaeus, A. georgiae, A. meyeri, A. neuii (both subspecies), and A. radingae being the identified species (25,64,92,107,175,205). In addition, A. turicensis has been isolated from necrotic tissue associated with cervicofacial fasciitis (107), and A. neuii has been isolated from a mammary hematoma (207).…”

Section: Superficial Infectionsmentioning

confidence: 99%

“…Abscesses are typically found in the groin, buttock, rectal area, and skin of the genitals. Other Actinomyces species commonly detected include A. europaeus, A. funkei, A. neuii (both subspecies), and A. radingae (25,47,64,107,114,175,178,202,209). It has been suggested that lipid-rich areas are favorable for the growth of these Actinomyces species (178).…”

Section: Superficial Infectionsmentioning

confidence: 99%

“…It has been suggested that lipid-rich areas are favorable for the growth of these Actinomyces species (178). Furthermore, A. europaeus, A. funkei, and A. turicensis have been isolated from decubitus ulcers (25,114,202); A. neuii and A. radingae have been isolated from diabetic ulcers (118,175); and A. europaeus, A. turicensis, and A. odontolyticus have been isolated from skin-related infections in lower extremities (25,202,210). It was suggested that the latter species, isolated from an intravenous drug abuser, originated from the oral cavity due to licking of an injection needle (210).…”

Section: Superficial Infectionsmentioning

confidence: 99%

“…Many Actinomyces species have been identified in soft tissue abscesses of the body above the waistline. Breast tissue is a site commonly affected by actinomycosis, with A. neuii especially being identified as a causative organism (11,64,119,175,(199)(200)(201). Also, A. europaeus (25,64,202,203), A. radingae (25,64,204), A. meyeri (92,205), and A. turicensis (25,204,206) have been recovered.…”

Section: Superficial Infectionsmentioning

confidence: 99%

See 4 more Smart Citations

Actinomyces and Related Organisms in Human Infections

2015

Self Cite

View full text Add to dashboard Cite

SUMMARY Actinomyces israelii has long been recognized as a causative agent of actinomycosis. During the past 3 decades, a large number of novel Actinomyces species have been described. Their detection and identification in clinical microbiology laboratories and recognition as pathogens in clinical settings can be challenging. With the introduction of advanced molecular methods, knowledge about their clinical relevance is gradually increasing, and the spectrum of diseases associated with Actinomyces and Actinomyces -like organisms is widening accordingly; for example, Actinomyces meyeri , Actinomyces neuii , and Actinomyces turicensis as well as Actinotignum (formerly Actinobaculum ) schaalii are emerging as important causes of specific infections at various body sites. In the present review, we have gathered this information to provide a comprehensive and microbiologically consistent overview of the significance of Actinomyces and some closely related taxa in human infections.

show abstract

Section: Eye Infectionsmentioning

confidence: 99%

Section: Superficial Infectionsmentioning

confidence: 99%

Section: Superficial Infectionsmentioning

confidence: 99%

Section: Superficial Infectionsmentioning

confidence: 99%

Section: Superficial Infectionsmentioning

confidence: 99%

See 3 more Smart Citations

Actinomyces and Related Organisms in Human Infections

2015

Self Cite

View full text Add to dashboard Cite

show abstract

Actinomyces timonensis sp. nov., isolated from a human clinical osteo-articular sample

Renvoisé

Raoult

Roux

2010

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Gram-positive, non-spore-forming rods were isolated from a human osteo-articular sample (strain 7400942 T ). Based on cellular morphology and the results of biochemical analysis, this strain was tentatively identified as a novel species of the genus Actinomyces. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the bacterium was closely related to the type strain of Actinomyces denticolens (96.9 % 16S rRNA gene sequence similarity). A comparison of biochemical traits showed that strain 7400942 T was distinct from A. denticolens in a number of characteristics, i.e. in contrast with A. denticolens, strain 7400942 T was negative for nitrate reduction and for b-galactosidase, a-glucosidase and alanine arylamidase activities, it was positive for acid production from N-acetylglucosamine, melezitose and glycogen, and it was negative for acid production from turanose. Matrix-assisted laser-desorption/ionization time-offlight MS protein analysis confirmed that strain 7400942 T represents a novel species, as scores obtained for its spectra were significant (.2.2) only with strain 7400942On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain should be designated Actinomyces timonensis sp. nov.; the type strain is strain 7400942The genus Actinomyces consists of high-G+C-containing, Gram-positive, anaerobic and aerotolerant, non-sporeforming, non-motile, rod-shaped micro-organisms. The natural habitats of members of the genus Actinomyces are the mucous membranes of humans and animals, particularly the oral mucosa, and urogenital and intestinal tracts (Acevedo et al., 2008). Mucosal disruption leads to infection. Most commonly, actinomycosis involves the cervicofacial area, followed by the abdominal and thoracic areas (Acevedo et al., 2008), but cutaneous, uterine, ocular, cerebrospinal and hepatic infections have also been described. Infection of bones is mainly due to adjacent tissue infection, but has also been described in some fractures or haematogenous spread. The lesions of actinomycosis are distinct from those observed in pyogenic osteomyelitis, multiple myeloma, fungal disease and osseous lesions of tuberculosis or metastatic cancer (Rothschild et al., 2006). Actinomyces naeslundii has been reported to cause hip prosthesis infection (Funke et al., 1997).Many studies have been performed to characterize Actinomyces representatives using phenotypic approaches (Santala et al., 2004;Miller et al., 1995; Kerttula et al., 2005), but their identification has remained problematic. Molecular approaches have been performed to detect, identify and clarify the taxonomic positions of putative pathogens (Hall et al., 2001;Woo et al., 2003;Song, 2005) and many novel species have been described from human or animal sources (Pascual et al., 1999; Hall et al., 2003aHall et al., , b, 2005An et al., 2006), principally based on 16S rRNA gene sequence comparisons and supported by other taxonomic tools (Hall, 2008).In this report, a strain of a novel species isolated from a h...

show abstract