Enzymatic Biofuel Cell for Oxidation of Glucose to CO<sub>2</sub>

Xu, Shuai

doi:10.1021/cs200523s

Cited by 118 publications

(93 citation statements)

References 14 publications

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“…Compared with the six-enzyme EFC that oxidizes glucose to CO 2 (ref. 13), the inherently low, but Supplementary Table S3. promiscuous, activities of one enzyme that catalyses several substrates results in very low power densities. The use of more than ten enzymes for implementing complex reactions for the production of biocommodities, fine chemicals and pharmaceuticals seems to be not economically prohibitive 25,26,[30][31][32][33] .…”

Section: Discussionmentioning

confidence: 99%

“…The incomplete oxidation of organic fuels mediated by one or a few enzymes in EFCs results in low energy densities, waste of fuel and inhibition of enzymes by the metabolic products. In vitro synthetic enzymatic pathways have been constructed on the anode compartments in EFCs for deep or complete oxidation of methanol 9,10 , ethanol 11 , glycerol 12 and glucose 13 . However, previous studies did not provide quantitative evidence (for example, Faraday efficiency) for the complete oxidation of organic fuels in microbial fuel cells 14,15 .…”

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confidence: 99%

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A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhu

Tam²,

Sun³

et al. 2014

Nat Commun

254

194

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High-energy-density, green, safe batteries are highly desirable for meeting the rapidly growing needs of portable electronics. The incomplete oxidation of sugars mediated by one or a few enzymes in enzymatic fuel cells suffers from low energy densities and slow reaction rates. Here we show that nearly 24 electrons per glucose unit of maltodextrin can be produced through a synthetic catabolic pathway that comprises 13 enzymes in an air-breathing enzymatic fuel cell. This enzymatic fuel cell is based on non-immobilized enzymes that exhibit a maximum power output of 0.8 mW cm À 2 and a maximum current density of 6 mA cm À 2 , which are far higher than the values for systems based on immobilized enzymes. Enzymatic fuel cells containing a 15% (wt/v) maltodextrin solution have an energy-storage density of 596 Ah kg À 1 , which is one order of magnitude higher than that of lithium-ion batteries. Sugar-powered biobatteries could serve as next-generation green power sources, particularly for portable electronics.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhu

Tam²,

Sun³

et al. 2014

Nat Commun

254

194

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“…Mediators have been used to enhance electron transfer rate [6,9,11] and carbon or mesoporous materials with diameters comparable to the size of the enzyme were explored for enhanced enzyme-electrode interactions [12]. The following approaches provide proper enzyme orientation: specific covalent attachment of enzymes to the electrode surface [13,14]; protein engineering …”

Section: Bottom Up Approach: Integration Of Biocatalysts With Differementioning

confidence: 99%

Bioelectrocatalysis and More

Babanova¹

2017

JNMR

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“…In 2011, Xu et al demonstrated the complete oxidation of glucose with a non-natural oxidation pathway. 6 A six-enzyme cascade was utilized to perform a 12-step, 24-electron oxidation of glucose. The power density showed an almost 50-fold increase with the cascade.…”

mentioning

confidence: 99%

“…In this work, we will demonstrate the detailed, mass spectrometric product analysis on a previously reported glucose oxidation system that utilizes a six-enzyme cascade to oxidize glucose to the final product, carbon dioxide. 6 PQQ-dependent glucose, gluconate, alcohol and aldehyde dehydrogenases, oxalate oxidase and aldolase were immobilized on carbon fiber electrodes to perform the complete oxidation of glucose (as shown in Figure 1). The biofuel cell operation products were quantitatively analyzed and the biofuel cell efficiencies were calculated, compared, and discussed.…”

mentioning

confidence: 99%

Characterizing Efficiency of Multi-Enzyme Cascade-Based Biofuel Cells by Product Analysis

2014

ECS Electrochemistry Letters

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The performance of biofuel cells with enzyme cascades have normally been characterized with open circuit potential, power density, and current density measurements. In this work, we demonstrate that with the method of quantitative product analysis by mass spectrometry, we can obtain other valuable information about the biofuel cell efficiency. Faradaic efficiency, coulombic efficiency and product efficiency were calculated for a six-enzyme glucose biofuel cell system. Oxidation pathway bottlenecks were determined with quantitative mass spectrometry measurements via direct infusion. These measurements and calculations give an in-depth understanding of the bioelectrocatalytic bottlenecks in the enzyme cascade for the target fuel (glucose 12 By mimicking the complete Krebs cycle on a carbon electrode, the power density was increased by 8.71-fold compared to a single enzyme-based ethanol biofuel cell. In 2009 and 2011, complete oxidations of other complex fuels (pyruvate 9 and lactate 10 ) with Krebs cycle enzymes were reported, which resulted in 26-fold enhancements in power density performance compared to a single enzyme-based biofuel cell. In 2011, Xu et al. demonstrated the complete oxidation of glucose with a non-natural oxidation pathway.6 A six-enzyme cascade was utilized to perform a 12-step, 24-electron oxidation of glucose. The power density showed an almost 50-fold increase with the cascade. Enhancements have also been observed for glucose in a bi-enzyme cascade 13 and for the deep and complete oxidation of maltodextrin. 14,15 Studies of the utilization of enzyme cascades in biofuel cell systems have demonstrated that deeper or complete oxidation of fuels can increase the power density and current density of biofuel cells. However, in order to have a better understanding on the performance of an enzyme cascade system, there are more questions to be answered: (1) How much of the electricity produced is from the designed oxidation pathway (faradaic efficiency)? (2) What ratio of the theoretical maximum amount of electrical energy in the fuel is produced during the biofuel cell operation process (coulombic efficiency)? (3) What percentage of the fuel is completely oxidized to the final product(s) (product efficiency)? To answer these questions, a careful analysis on the intermediates and products of the biofuel cell systems needs to be carried out. Product analysis not only gives the quantitative analysis needed to calculate the different types of efficiencies mentioned above, but also provides evidence to investigate the bottleneck step(s) in the oxidation pathway which is crucial information for bioanode cascade optimization and, therefore, fuel cell performance optimization.Faradaic efficiency, coulombic efficiency, and product efficiency describe different aspects of biofuel cell performance. Faradaic effi- * Electrochemical Society Fellow.z E-mail: minteer@chem.utah.edu ciency is defined as the fraction of charge passed to form intermediates and product(s) in an electrochemical process divided by the to...

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Enzymatic Biofuel Cell for Oxidation of Glucose to CO₂

Cited by 118 publications

References 14 publications

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Bioelectrocatalysis and More

Characterizing Efficiency of Multi-Enzyme Cascade-Based Biofuel Cells by Product Analysis

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Enzymatic Biofuel Cell for Oxidation of Glucose to CO2

Cited by 118 publications

References 14 publications

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Bioelectrocatalysis and More

Characterizing Efficiency of Multi-Enzyme Cascade-Based Biofuel Cells by Product Analysis

Contact Info

Product

Resources

About

Enzymatic Biofuel Cell for Oxidation of Glucose to CO₂