Enzymatic Biosensor Based on One‐step Electrodeposition of Graphene‐gold Nanohybrid Materials and its Sensing Performance for Glucose

Abstract

RGO/Au/Ni electrode was manufactured by a convenient, controllable, and environmental process, which was carried out by cyclic voltammetry (CV), and in this process, graphene-gold nanohybrid materials were simultaneously deposited on the nickel foam. Then the GOx was immobilized on the RGO/Au/Ni electrode by covalent bonding, and obtained the enzymatic biosensor. Scanning electron microscope (SEM) and Raman spectroscopy were adopted to confirm the microstructure of the fabricated RGO/Au/Ni electrode. Fourier transform infra-red spectroscopy (FT-IR) was used to characterize the prepared enzymatic electrode. CV, chronoamperometry, and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical performance of the fabricated enzymatic biosensor. It is found that AuNPs were well dispersed on the wrinkled RGO sheets, and the biosensor had a high sensitivity to glucose (32.83 μA • mM À 1 • cm À 2 ) with a wide linear range (0.15�26.15 mM), the strengths of anti-interference ability, good stability, and repeatability, etc.

Fabricating MOF‐derived Co3O4 Materials with Co‐catalysis Centers Matching 2‐reaction Sites of Dopamine for Extremely Low Limit of Detection

“…In biological fluid there are various interference species such as UA and AA that have very close oxidation potential with that of DA. Some commonly existing interferences including AA, KCl, UA, NaCl, H 2 O 2 , glucose are selected to examine the selectivity of HS−Co 3 O 4 sensor [33, 34]. DPV curves in Figure 4c displays the various interferences in 10 μm DA have no significant signals but only DA gives an extremely high response on HS−Co 3 O 4 sensor, indicating that all AA, KCl, UA, NaCl, H 2 O 2 and glucose have negligible interference signals and thereby solidly confirming excellent selectivity of the sensor.…”

“…In biological fluid there are various interference species such as UA and AA that have very close oxidation potential with that of DA. Some commonly existing interferences including AA, KCl, UA, NaCl, H 2 O 2 , glucose are selected to examine the selectivity of HSÀ Co 3 O 4 sensor [33,34]. DPV curves in Figure 4c displays the various interferences in 10 μm DA have no The stability of the sensor was also examined by testing after 14 days of its initial measurement as in Figure S3a, displaying that the sensor response only drops by 3.21 %, further verifying its good stability.…”

Effect of Modification of a Laccase-Based Electrochemical Biosensor with Carbon Nanotubes on Signal Separation of Dihydroxybenzene Isomers

“…16 Traditionally, laccase biosensors are obtained by conventional "wet" methods, which involve immobilization of the laccase enzyme on the electrode surface due to, e.g., covalent bond formation. 17 It very often needs a long-hour immersion of the electrochemical transducer in an enzyme solution to bond with the electrode surface. Therefore, the applicability of this approach in biosensor construction is significantly limited.…”