Enzymatic Blood Glucose Determination by Colorimetry of N,N-Diethylaniline-4-Aminoantipyrine

Kabasakalian, Peter; Kalliney, Sami; Westcott, Anita

doi:10.1093/clinchem/20.5.606

Cited by 65 publications

(15 citation statements)

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“…Overall, the pores can behave as micro/nano-reactors with a relevant chemical entity, such as a catalyst, firmly anchored inside and the proper reactant molecules can reach the pores through the polymer mesh by diffusion, feeding in this way the reaction. To test this hypothesis, we made a preliminary experiment by loading free glucose oxidase enzyme, GOX (Figure 7a,b), enzymatic activity was tested by colorimetric assay [52]. Remarkably, the monolithic porous material loaded with GOX (1 U/mL) and PANI (0.8% wt) retained the enzymatic activity towards glucose oxidation to glucanolactone (Figure 7c).…”

Section: Resultsmentioning

confidence: 99%

Open Porous Composite Monoliths for Biomedical Applications via Photocrosslinking of Low Internal Phase Nano-Emulsion Templates

et al. 2021

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Highly cross-linked polyethylene glycol monoliths (HCPEG) with interconnected micro and nanoporosity are produced via photo-crosslinking of low internal phase emulsions (LIPE). Unlike previous works, this approach allows the pre-processing functionalization of both polymer matrix and porosity by loading both phases of the emulsion template with several active fillers, such as enzymes, semiconductive polymers, and metallic nanostructures. Importantly, both polymer matrix and porosity of the resulting composite HCPEG monoliths show neither serious cross-contamination nor morphological alterations. All in all, this material behaves like a network of nano/micro flasks embedded into a permeable media. Mechanical and dielectric properties of these composites HCPEG monoliths can be tuned by varying the content of fillers. Since these composite materials are produced by photo-crosslinking of LIPEs, they can be easily and rapidly processed into complex shapes like microneedles arrays through replica molding without detrimental modifications of the porous morphology. In principle, the proposed strategy allows us to fabricate medical devices. As proof of concept, we embedded glucose oxidase enzyme in the nanoporosity and the resulting composite porous material retained the catalytic activity towards the oxidation of glucose.

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Section: Resultsmentioning

confidence: 99%

Open Porous Composite Monoliths for Biomedical Applications via Photocrosslinking of Low Internal Phase Nano-Emulsion Templates

et al. 2021

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“…The 4‐aminoantipyrine motif is a ubiquitous feature of natural products and is also a metabolite of aminopyrine with analgesic. Also, it is used as a reagent for biochemical reactions producing peroxides or phenols, and to measure extracellular water . Based on the above reports, we explored the cross‐coupling reaction of 4‐aminoantipyrine ( 6 ) with aryl iodides.…”

Section: Resultsmentioning

confidence: 99%

Ligand‐free CuTC‐catalyzed N‐arylation of amides, anilines and 4‐aminoantipyrine: synthesis of N‐arylacrylamides, 4‐amido‐N‐phenylbenzamides and 4‐amino(N‐phenyl)antipyrenes

Quan

Xia

Zhang

et al. 2013

Applied Organom Chemis

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N-Arylation of amides and anilines with aryl iodides was efficiently catalyzed by copper thiophenecarboxylate under ligandfree conditions with good to excellent yields. A variety of substituted aryl iodides, amides, anilines and 4-aminoantipyrine were found to be applicable to the simple catalytic system. Furthermore, some practical, unique secondary amides, such as N-arylacrylamides and 4-amido-N-phenylbenzamides, and 4-amino(N-phenyl)antipyrenes, which are difficult to obtain by the classical methods, were prepared. a Reaction conditions: iodobenzene (1a, 1.0 mmol), acrylamide (2a, 1.2 mmol), base (2.0 mmol), solvent (3.0 ml), under nitrogen. b Isolated yield. c H 2 O (1.0 mmol) was added to the reaction. Z.-J Quan et al. wileyonlinelibrary.com/journal/aoc Additional supporting information may be found in the online version of this article at the publisher' web-site. Scheme 1. A possible mechanism for the CuTC-catalyzed N-arylation.Ligand-free CuTC-catalyzed N-arylation Appl.

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“…Glucose levels were measured by enzymatic colorimetry on a UV-VIS spectrophotometer at 500 nm using a glucose liquicolor kit. The absorbance obtained was compared with the absorbance of the blank control and then multiplied by the standard concentration [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Binahong (Anredera cordifolia (Tenore) Steen.) Leaf Extract Modulates Fatty Acids and Amino Acids to Lower Blood Glucose in High-Fat Diet-Induced Diabetes Mellitus Rats

Dwitiyanti

Harahap

Elya

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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Patients with diabetes are 1.6 times more likely to use complementary alternative medicine than nondiabetic patients. Previous studies have shown that Anredera cordifolia (Tenore) Steen. (A. cordifolia) leaf extract has the capacity to lower blood glucose, but the actual mechanisms are unclear. Therefore, in this study, we explored the effect of A. cordifolia leaf extract on the metabolism of fatty acids and amino acids. Six-week-old male Wistar rats were randomly divided into six experimental groups (n = 5 per group). Two groups were fed with a regular diet or a high-fat diet (HFD) for six weeks. The regular diet and HFD groups were administered with 0.5% carboxymethylcellulose as a vehicle, and HFD rats were also fed with a suspension of glibenclamide (0.51 mg/kg body weight (BW)) or A. cordifolia leaf extract (25, 50, and 100 mg/kg BW). During the whole treatment, BW and food intake were recorded weekly. The rats were euthanized seven weeks after treatment. Blood glucose was evaluated by spectrophotometry, while fatty acids and amino acids were evaluated using a gas chromatography/flame ionization detector (GC/FID). All doses of A. cordifolia administration reduced blood glucose significantly, and 50 mg/kg BW was most effective in lowering blood glucose, similar to the effects of glibenclamide. A. cordifolia leaf extract affected the levels of medium-chain fatty acids, especially at 50 mg/kg BW. In contrast, glibenclamide affected long-chain fatty acids (LCFAs) to lower blood glucose. Based on the analysis conducted, we conclude that administration of A. cordifolia leaf extract can decrease blood glucose levels by regulating fatty acid metabolism and that a dose of 50 mg/kg BW in rats was the optimal dose.

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Enzymatic Blood Glucose Determination by Colorimetry of N,N-Diethylaniline-4-Aminoantipyrine

Cited by 65 publications

References 0 publications

Open Porous Composite Monoliths for Biomedical Applications via Photocrosslinking of Low Internal Phase Nano-Emulsion Templates

Open Porous Composite Monoliths for Biomedical Applications via Photocrosslinking of Low Internal Phase Nano-Emulsion Templates

Ligand‐free CuTC‐catalyzed N‐arylation of amides, anilines and 4‐aminoantipyrine: synthesis of N‐arylacrylamides, 4‐amido‐N‐phenylbenzamides and 4‐amino(N‐phenyl)antipyrenes

Binahong (Anredera cordifolia (Tenore) Steen.) Leaf Extract Modulates Fatty Acids and Amino Acids to Lower Blood Glucose in High-Fat Diet-Induced Diabetes Mellitus Rats

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