Enzymatic catalysis in cosolvent modified pressurized organic solvents

Sarkari, Marazban; Knutson, Barbara L.; Chen, Ching‐Shih

doi:10.1002/(sici)1097-0290(19991105)65:3<258::aid-bit2>3.0.co;2-a

Cited by 9 publications

(2 citation statements)

References 35 publications

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“…So the concentration of added salt hydrates should be kept minimal. According to the work by Ching-Shih Chen [17], the common addition concentration is between 5-50 g/l. So the concentration gradients were set as 0.1, 1, 10, 100, and 500 g/l.…”

Section: Effect Of Addition Concentration Of Salt Hydratesmentioning

confidence: 99%

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Xia

Wang

Yang

et al. 2009

Appl Biochem Biotechnol

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Eleven lipases are tested and it was found that lipases can be divided into three types according to water activity dependence. The first type is lipase that has low water activity dependence and works in a low water activity, its performance changes little with the change of water activity. The optimum water activity is 0.19 and Newlase F (Rhizopus niveus), lipase FAP-15 (Rhizopus oryzae) belong to this type. The second type is lipase that has medium water activity dependence and its performance changes with the change of water activity. Most lipases belong to this type and the optimum water activity in this type is about 0.60. The third type is lipase that has a high water activity dependence and works only in a high water activity (a ( w ) > 0.75). WGL (wheat germ) belongs to this type and the optimum water activity is 0.90. The relationship between enantioselectivity and water activity is also discussed and the enantioselectivity seems to be independent of water activity. And we also compared the two control methods of water activity, it was found that the method which add solid salt hydrates to the reaction mixture (method II) is more stable and effective throughout the reaction than the method that pre-equilibrate via the vapor phase (method I). The addition concentration of salt hydrates is also investigated and the optimum concentration is 1 g/l.

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Section: Effect Of Addition Concentration Of Salt Hydratesmentioning

confidence: 99%

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Xia

Wang

Yang

et al. 2009

Appl Biochem Biotechnol

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“…There are also several applications for the SCF technology in food engineering including: extraction of compounds from natural products (the processing of hops, the extraction of caffeine, vanilla, beta-carotene, and vegetable oils), food sterilization, removal of undesired extractable (pesticides residues, hazardous chemicals from fish tissue, oil from dry-milled corn germ), and fractionation of cod liver oil (Bruno et al, 1993). Catalytic reactions in supercritical CO 2 have been receiving an increased attention during the last decade (Sarkari et al, 1993). This chapter has focused on SCF special applications in the field of food biotechnology.…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid Application in Food and Bioprocess Technology

Mozafari¹

2011

Mass Transfer - Advanced Aspects

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Technical aspects of biocatalysis in non‐CO₂‐based supercritical fluids

Karmee

Casiraghi

Greiner

2008

Biotechnology Journal

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The number of biotransformation processes is increasing rapidly. Part of this success is based on the inherent properties of enzymes as chemo-, regio-, and enantioselective catalysts. Supercritical fluids (scF) are superior solvents inheriting adjustable and partly unique physical properties. These can be advantageously combined with biotransformations, as solvent power responds to pressure and temperature changes according to the reaction requirements. Among the scF, supercritical carbon dioxide has undoubtedly gained the highest attention. However, other scF are also recognized to enlarge the possibilities. Among these CH(4), C(2)H(6), C(2)H(4), C(3)H(8), CHF(3), and SF(6) are used as scF for biocatalysis. This review focuses on the use of non-CO(2) based scF for biotransformations. Wherever possible, special emphasis is given on the industrial viability of different biocatalytic processes.

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Enzymatic catalysis in cosolvent modified pressurized organic solvents

Cited by 9 publications

References 35 publications

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Supercritical Fluid Application in Food and Bioprocess Technology

Technical aspects of biocatalysis in non‐CO₂‐based supercritical fluids

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Enzymatic catalysis in cosolvent modified pressurized organic solvents

Cited by 9 publications

References 35 publications

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Supercritical Fluid Application in Food and Bioprocess Technology

Technical aspects of biocatalysis in non‐CO2‐based supercritical fluids

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Product

Resources

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Technical aspects of biocatalysis in non‐CO₂‐based supercritical fluids