Enzymatic Cellulose Palmitate Synthesis Using Immobilized Lipase

Abstract

Bacterial cellulose can be modified by esterification using palmitic acid and Mucor miehei lipase as catalysts. The purpose of this research was to determine the optimum conditions of esterification reaction of cellulose and palmitic acid. The esterification reaction was carried out at the time variation of 6, 12, 18, 24 and 30 hours and the mass ratio of cellulose: palmitic acid (1:1, 1: 2, 1: 3, 1:4, 1:5, 1:6) at 50°C. The cellulose palmitate was examined its physical and chemical properties by using FTIR spectrophotometer, XRD, bubble point test and saponification apparatus. The results showed that the optimum reaction time of esterification reaction of cellulose and palmitic acid occurred within 24 hours and the mass ratio of cellulose: palmitic acid was 1:3 resulting in DS of 0.376 with swelling index of 187%, crystallinity index of 61.95%, and Φ porous of 2.40 μm. Identification of functional groups using FTIR spectrophotometer showed that C=O ester group was observed at 1737.74 cm-1 and strengthened by the appearance of C-O ester peak at 1280 cm-1. The conclusion of this study is reaction time and reactant ratio influence significantly the DS of cellulose ester.

Characteristic of Cellulose Isolated From Papyrus Fibers (Borrasus flabelifer L) And Its Citrate Ester

“…The DS value of cellulose ester citric from esterification process with 0.6 M citric acid at various temperatures shows in figure 3. The effect of the temperatures is the important aspect of esterification process because the esterification reaction should be carried out in a free water state [14]. Based on the figure, the DS value was increased from 110 o C to 120 o C and slightly decrease and become stable at the temperature above 120 o C. The DS value was sharply increased from 0.341 to 1.147.…”

High-Solids, Solvent-Free Modification of Engineered Polysaccharides

“…The particular film is still used today in the packaging sector, but it is constantly being replaced by alternative, lower-cost, melt-processable materials, which are meanwhile manufactured by lower-emission processes [ 6 ]. For example, thermoplastic starch is used for the manufacture of packaging films and drug capsules [ 12 , 13 , 14 ], while cellulose ester derivatives are used in membranes, films and fibers [ 7 , 15 ]. Nevertheless, the properties of these materials are dominated by the fixed structure of the initial natural polysaccharide feedstock, and any adjustments of that underlying structure in a systematic approach have not been possible.…”