Enzymatic Composition of Canine Leg Lymph

Lindena, J.; Küpper, Werner; Trautschold, I.

doi:10.1159/000459080

Cited by 10 publications

(14 citation statements)

References 14 publications

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“…Lymph-plasma ratios in excess of unity indicate that the molecule derives primarily from the interstitial space. We recently introduced choline esterase äs a more reliable marker than plasma prpteins (21). Choline esterase is exclusively synthesised in liver.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma

Lindena¹,

Küpper²,

Trautschold³

1982

CCLM

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Lindena, Küpper and Träutschold: Skeletal muscle hypoxia and enzyme activities in lymph and plasma 95 J. Gin. Chem. Clin. Biochem. Vol. 20,1982, pp. 95-102 (Received July 7/October 21,1981) Summary: The effect of hypoxia lasting one hour on the hind leg muscle of anaesthetised dogs was investigated. Ten enzyme activities in plasma and leg lymph, and the lymphatic transport of these enzymes were investigated. Enzymes with high activity in muscle, like creatme kinase, lactate dehydrogenase, malate dehydrogenase and adenylate kinase only show an increase in the plasma, if lymph -propulsed by passive motion of the hind leg -can reach the intravascular space. This effect is independent of transient hypoxia. Depending on the level of enzyme activity in the muscle, the activity in leg lymph is up to 6-föld higher than in plasma. Enzymes from muscle have to be transported into the blood by lymph flow and not via a direct interstitial-venous entry. The results are discussed especially with respect to enzyme activity changes in plasma during physical exercise.

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Section: Discussionmentioning

confidence: 99%

“…Hitherto, however, lymph seems to be a forgotten organ and only a few grpups have paid attention to the effect of physical activity on enzyme activities in this extracellular fluid compartment (10,(21)(22)(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma

Lindena¹,

Küpper²,

Trautschold³

1982

CCLM

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“…We know today that both enzymes are found in plasma in nearly the same order of magnitude, whereas in skeletal muscle the catalytic activity of creatine kinase is 5 -10-fold higher than for lactate dehydrogenase (20,66).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the methods described in a previous publication (20), the following methods were applied for the following enzymes: Glucose-6-phosphate dehydrogenase according to Löhr &. Waller (21) (Boehringer, Mannheim).…”

Section: Determination öF Catalytic Enzyme Activity Concentrationmentioning

confidence: 99%

The Decline of Catalytic Enzyme Activity Concentration of In Vivo Ageing Erythrocytes of the Man, the Dog and the Rat. Approach to a Quantitative Diagnostic Enzymology, IV. Communication

Lindena¹,

Wittenberg²,

Diederichs³

et al. 1986

Clinical Chemistry and Laboratory Medicine

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Summary:Human, dog and rat erythrocytes were separated by centrifugation on a discontinuous buffered Percoll gradient into fractions of progressively increasing mean cell age to measure the in vivo decline in catalytic activity of eleven enzymes during the erythrocyte lifespan. Erythrocyte enzymes decline exponentially at different rates and also differ between the species. The maximal and minimal catalytic activities (erythrocyte catalytic activity at the beginning and at the end of the appröpriate erythrocyte life-span for a given species) and the intracellular half-life of enzymes were estimated. To test the hypothesis that circulating erythrocytes inake a significant contribution to the normal catalytic activity in plasma it was assumed äs a working hypothesis that the measured loss of catalytic activity in ageing erythrocytes is equivalent to the amount of the enzymes released in catalytically active form into plasma. This contribution was calculated. Die Abnahme der katalytischen Enzymaktivitätskonzentration von in vivo alternden Erythrocyten bei Mensch, Hund und Ratte Versuch der Begründung einer quantitativen Diagnostischen Enzymologie, IV.Zusammenfassung: Erythrocyten von Mensch, Hund und Ratte wurden in einem diskontinuierlichen PercollGradienten in Puffer in Fraktionen zunehmenden Alters separiert, um den in vivo-Abfall der katalytischen Aktivität von 11 Enzymen über die entsprechende Erythrocyten-Lebensdauer zu bestimmen. Die einzelnen Enzyme verlieren ihre kätalytische Aktivität exponentiell .mit unterschiedlicher Rate, auch unterschiedlich bei den untersuchten Spezies. Die maximalen und minimalen katalytischen Aktivitäten (kätalytische Aktivität der Erythrocyten zu Beginn und zum Ende der entsprechenden Erythrocyten-Lebensdauer der untersuchten Spezies) und die intrazelluläre Halbwertszeit der Enzyme wurden bestimmt. Um die Hypothese zu testen, daß zirkulierende Erythrocyten einen wesentlichen Beitrag zur normalen katalytischen Enzymaktivität des Plasmas leisten, wird als Arbeitshypothese angenommen, daß der Verlust des alternden Erythrocyten an katalytischer Aktivität gleichbedeutend ist mit einer Freisetzung an katalytisch aktivem Enzym in das Plasma. Dieser Beitrag wird berechnet.

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“…For anaesthetized rats the only reported data are severely affected by methods of sample preparation (69). For mouse thoracic duct lymph, we recently reported selected catalytic activities in lymph and plasma of short-term fistulized animals (70).…”

Section: Thoracic Ductmentioning

confidence: 99%

Catalytic Enzyme Activity Concentration in Thoracic Duct, Liver, and Intestinal Lymph of the Dog, the Rabbit, the Rat and the Mouse. Approach to a Quantitative Diagnostic Enzymology, II. Communication

Lindena¹,

Küpper²,

Trautschold³

1986

Clinical Chemistry and Laboratory Medicine

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Summary:In the mixed body lymph of the thoracic duct and in the defined organ lymph of the liver and the intestine, the catalytic activity concentrations of up to sixteen enzymes arid the concentrations of albumin and protein were determiiied, äs well äs the transport rate of these substances and their lymph/plasma ratio. Thoracic duct lymph specimens were obtained from an extracorporeal lymph shunt in anaesthetized and conscious dogs and from short-term fistulas in anaesthetized rabbits, rats and mice. Additionally, rabbits and rats underwent passive motion of the bind limbs in another experimental trial. Thoracic duct flow in anaesthetized dogs is only half that seen in conscious dogs, due to bypassed muscular lymph. A similar flow change is seen during passive motion of bind limbs in anaesthetized rabbits and rats. From a literature review of flow in the four main lymphatics of the body, it is concluded that the thoracic duct flow should account for 50-70% of total body lymph flow. In the anaesthetized stäte, flow is mainly of visceral origin. In the conscious state and during passive motion the ihäteased flow is of miisfcular origin. In the latter case, the catalytic activities of enzymes like lactate dehydrogefiase, maläte döhydfogenase, creatine kinase, aldolase and phosphohexose isomerase, increase in lymph äs doels their lympH/plasma ratio. These enzymes have high catalytic activities in muscle* Their transport into the blöod increases 2-3-fold, due tö a doubling of l^mph flow. Reported data for anaesthetized and immobile animals theffcfore far tinderestimate the significahce of thoracic duct enzyme transport. Liver lymph was obtaihed frdftl anaesthetized dogs and rabbits. Our Unding that lymph catalytic activity for several enzymes is higher than in plasma iS not compatible with the proposed delivery of plasma proteins directly into the sinusöidäl space without priör ffl&ing with the Space of Disse. Enzymes in liver lymph should derive from parenchymal afld endothelial liiiiflg Sfells. Their site of delivery from the hepatocyte seems different from that of proteins. Liver lymph is an important transport route of enzymes into the blood. Intestinal lyiflßh was sampled from anaesthetized dogs, rabbits and rats. It was shown that most enzymes from the intestine are primarily released into the interstitial space and from there aie transported via the lymph into the blood. Thoracicus-, Leber-und Katalytische Enzymaktivitätskonzentration in Ductus

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Enzymatic Composition of Canine Leg Lymph

Cited by 10 publications

References 14 publications

Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma

Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma

The Decline of Catalytic Enzyme Activity Concentration of In Vivo Ageing Erythrocytes of the Man, the Dog and the Rat. Approach to a Quantitative Diagnostic Enzymology, IV. Communication

Catalytic Enzyme Activity Concentration in Thoracic Duct, Liver, and Intestinal Lymph of the Dog, the Rabbit, the Rat and the Mouse. Approach to a Quantitative Diagnostic Enzymology, II. Communication

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