Enzymatic degradation of biofilm by metalloprotease from<i>Microbacterium</i>sp. SKS10

Saggu, Sandeep Kaur; Jha, Gopaljee; Mishra, Prakash Chandra

doi:10.1101/655092

Cited by 3 publications

(4 citation statements)

References 36 publications

(23 reference statements)

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“…and a Brevibacillus brevis isolate [58,59]. Three of our isolates, on the other hand, showed highest preference for casein which has been commonly observed in previous studies as well [60][61][62][63][64].…”

Section: Discussionsupporting

confidence: 82%

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Hossain

Das

Ali

et al. 2021

Preprint

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Vertebrate intestine appears an excellent source of proteolytic bacteria for industrial and probiotic use. We therefore aimed obtaining the gut-associated proteolytic species of Nile tilapia. We have isolated twenty six bacterial strains from its intestinal tract, seven of which showed exoprotease activity with the formation of clear halos on skim milk. Their depolymerization ability was further assessed on three distinct proteins including casein, gelatin and albumin. All the isolates could successfully hydrolyze the three substrates indicating relatively broad specificity of their secreted proteases. Molecular taxonomy and phylogeny of the proteolytic isolates were determined based on their 16S rRNA gene barcoding which suggested that the seven strains belong to three phyla viz. Firmicutes, Proteobacteria and Actinobacteria, distributed across the genera Priestia, Citrobacter, Pseudomonas, Stenotrophomonas, Burkholderia, Providencia and Micrococcus. The isolates were further characterized by a comprehensive study of their morphological, cultural, cellular and biochemical properties which were consistent with the phylogenetic annotations. To reveal their proteolytic capacity alongside substrate preferences, enzyme-production was determined by the diffusion assay. The Pseudomonas, Stenotrophomonas and Micrococcus isolates appeared most promising with maximum protease production on casein, gelatin and albumin media respectively. Our findings present valuable insights into the phylogenetic and biochemical properties of gut-associated proteolytic strains of Nile tilapia.

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Section: Discussionsupporting

confidence: 82%

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Hossain

Das

Ali

et al. 2021

Preprint

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“…More EPS production translates into a thicker barrier for disinfectants to bypass before contact with underlying bacteria. Additionally, a thicker EPS matrix may also result in a pH gradient, which can impact bactericidal efficacy [20]. These factors could account for the reduced bactericidal efficacy against 72 h DSB of P. aeruginosa compared to 72 h DSB of S. aureus.…”

Section: Bactericidal Efficacy Varies By Species After Prolonged Dehy...mentioning

confidence: 99%

“…45.6 µm) [21]. Considering biofilm thickness also influences biofilm architecture, this may result in aggregation of organic acids leading to the deactivation or reduced performance of disinfectants [20]. While these laboratorygrown models offer a closer representation of clinical biofilms, there is still limited work evaluating the efficacy of disinfectants in removing biofilm formed on dry clinical surfaces at different dehydration time points under near room-temperature conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The EPS forms a matrix that presents a major barrier to removal from surfaces in healthcare facilities as it shields underlying bacterial cells from direct contact with disinfectants used for their inactivation [17,18]. Biofilm thickness likely influences several factors such as the disinfectant ability to penetrate the biofilm matrix [19], the water-binding characteristic of the EPS matrix [14], and pH differences among various layers of biofilms [20]. While WSBs could vary in terms of thickness compared to artificially produced DSB or DSB collected from environmental samples, a depth of 31 µm of laboratory-developed DSB models represents a reasonable compromise between the thickness of biofilms collected from hard surfaces (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums

Chaggar

Nkemngong

Li³

et al. 2022

Microbiology

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Globally, healthcare-associated infections (HAI) are the most frequent adverse outcome in healthcare delivery. Although bacterial biofilms contribute significantly to the incidence of HAI, few studies have investigated the efficacy of common disinfectants against dry-surface biofilms (DSB). The objective of this study was to evaluate the bactericidal efficacy of seven Environmental Protection Agency (EPA)-registered liquid disinfectants against DSB of Staphylococcus aureus and Pseudomonas aeruginosa . We hypothesized that overall, there will be significant differences among the bactericidal efficacies of tested disinfectants by product type and active ingredient class. We also hypothesized that depending on the species, higher bactericidal efficacies against DSB will be exhibited after 24 h of dehydration compared to 72 h. Wet-surface biofilms of S. aureus and P. aeruginosa were grown following EPA-MLB-SOP-MB-19 and dehydrated for 24 and 72 h to establish DSB. Seven EPA-registered disinfectants were tested against dehydrated DSB following EPA-MLB-SOP-MB-20. Overall, quaternary ammonium plus alcohol, sodium dichloro-s-triazinetrione and hydrogen peroxide products were more efficacious against DSB than quaternary ammoniums for both tested species. While there was no significant difference in the log10 reductions between 24 and 72 h S . aureus biofilms, significantly higher log10 reductions were observed when products were challenged with 24 h P . aeruginosa DSB compared to 72 h P . aeruginosa DSB. Species type, active ingredient class and dry time significantly impact disinfectant efficacy against DSB of S. aureus or P. aeruginosa .

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Bacterial biofilms: role of quorum sensing and quorum quenching

GHOSH¹,

SETH²,

Mondal³

et al. 2022

J Exp Bio & Ag Sci

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Bacterial biofilms provide an adjustable strategy to manage themselves in the existing conditions. Biofilms of pathogenic bacteria act as a reservoir for various device and non-device related diseases which are tough to cure. Exposure to a high dose of antibiotics is not an appropriate solution to this problem as high antibiotic concentrations lead to the generation of Multi-drug resistant strains as well as affect the human body. So, it is needed to bypass the use of antibiotics to prevent bacterial biofilms. In this context, Quorum Sensing (QS) may be a potential target since biofilm formation is regulated by QS. N-acyl homoserine lactones (N-AHL) act as predominant QS signal molecules in Gram-negative bacteria. Counteraction of the QS-regulated activities using quorum quenching may be an alternative way to combat biofilm formation in bacteria. Quorum sensing inhibitors (QSIs) and QQ enzymes play a significant role in this regard either by interference with the signal generation, perception, or by degradation, and chemical modification, respectively. Many quorum quenching enzymes have been reported from bacteria. Extremophilic bacteria have also been reported to produce potent quorum quenching enzymes which can effectively break down N-AHLs.

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Enzymatic degradation of biofilm by metalloprotease fromMicrobacteriumsp. SKS10

Cited by 3 publications

References 36 publications

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums

Bacterial biofilms: role of quorum sensing and quorum quenching

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