Enzymatic degradation of poly (ε-caprolactone) and cellulose acetate blends by lipase and α-amylase

Calil, M.R.; Gaboardi, F.; Bardi, Marcelo Augusto Gonçalves; Rezende, M.L.; Rosa, Derval dos Santos

doi:10.1016/j.polymertesting.2006.10.007

Cited by 27 publications

(12 citation statements)

References 17 publications

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“…Embora no estudo in vitro tenham sido empregadas condições que se aproximam das fisiológi-cas, é de se esperar que o comportamento seja diferente no estudo in vivo, já que a presença de processos metabólicos exerce uma importante influência no mecanismo de liberação do fármaco (22)(23) . A partir do estudo realizado, pode-se observar que o AP foi liberado dos sistemas administrados aos animais e que concentrações significativas do fármaco foram encontradas nos principais tecidos envolvidos nas orbitopatias inflamatórias.…”

Section: Discussionunclassified

Sistemas biodegradáveis contendo acetato de prednisolona para administração orbitária

Byrro¹,

Miyashita²,

Albuquerque³

et al. 2009

Arq. Bras. Oftalmol.

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Section: Discussionunclassified

Sistemas biodegradáveis contendo acetato de prednisolona para administração orbitária

Byrro¹,

Miyashita²,

Albuquerque³

et al. 2009

Arq. Bras. Oftalmol.

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“…Recently, such catalyzed reactions have been progressively used to biodegrade various synthetic polymers through the enzymes produced by microorganisms. [25][26][27][28][29][30][31] Enzymes are amino acid molecules and their function is to catalyze various chemical reactions in biological environments, e.g., in the human body or animals. The rate of most enzymecatalyzed reactions is millions of times faster than those of comparable uncatalyzed reactions.…”

Section: Enzymatically-induced Hydrolysismentioning

confidence: 99%

A mechanistic study of degradation of a typical automotive clearcoat caused by bird droppings

2010

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This study aims at investigating the degradation mechanism of an automotive clearcoat caused by bird droppings. Natural bird droppings and their synthetic equivalent (pancreatin) were utilized for this purpose. The presence of highly-etched areas on the surface of coatings and structural variations of clearcoat after being attacked by these materials corresponded to a catalyzed hydrolytic degradation. This finding was obtained using different analytical techniques. Based on these studies, three possible hypotheses were presented, including acid catalysis, metal ion catalysis, and enzymatic catalysis. The conditions required for the occurrence of each hypothesis were discussed. It was found that acid and metal ions have a weak contribution to catalyze the hydrolysis reaction of clearcoat, whereas enzymes existing in bird droppings were mainly responsible for this hydrolytic degradation.

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“…Here poly(-caprolactone) (PCL) film was chosen as cell growth matrix due to its excellent biocompatibility and biodegradability [25][26][27][28][29][30]. Importantly, PCL has been approved by the USA Food and Drug administration (FDA) and can be used in human body [7,[31][32][33].…”

Section: Introductionmentioning

confidence: 99%

In situ evaluation of cell cultivation on dynamically changed poly(ɛ-caprolactone) film caused by enzymatic degradation

Jiang

Gan

2011

Sci. China Chem.

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In situ evaluation of cell cultivation on degrading poly(-caprolactone) (PCL) films was studied. New culture surroundings were constructed for cell growth by using PCL films as substrates and adding Pseudomonas cepacia lipase to accelerate biodegradation of PCL films. MTT experiments for 10 h indicated the low cytotoxicity of lipase solution with concentration up to 0.2 mg/mL for MG-63 cells growth on PCL films. With the optimized lipase concentration and degradation time, we studied cell growth behavior on dynamically changed PCL films by adding lipase to the culture surroundings. MTT, fluorescence microscopy and scanning electron microscopy (SEM) were used to evaluate cell viability, proliferation and morphologies. It was found that cell viability and proliferation were not affected by the added lipase solution negatively. In contrast, cells cultured on degrading PCL films showed good growth behavior with clear fusiform shape and pseudopods. Importantly, the enzymatic degradation of PCL films with cells attachment showed distinctive morphology compared to the degradation in lipase solution without cells. The simultaneous cell growth and PCL film degradation were well discussed in this work, which may better understand the interaction between cell growth and polymer degradation.poly(-caprolactone) film, enzymatic degradation, cell growth, surface morphology

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Enzymatic degradation of poly (ε-caprolactone) and cellulose acetate blends by lipase and α-amylase

Cited by 27 publications

References 17 publications

Sistemas biodegradáveis contendo acetato de prednisolona para administração orbitária

Sistemas biodegradáveis contendo acetato de prednisolona para administração orbitária

A mechanistic study of degradation of a typical automotive clearcoat caused by bird droppings

In situ evaluation of cell cultivation on dynamically changed poly(ɛ-caprolactone) film caused by enzymatic degradation

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