Enzymatic Degradation of Pretreated Pig Bristles with Crude Keratinase of Bacillus cereus PCM 2849

Łaba, Wojciech; Chorążyk, Dorota; Pudło, Anna; Trojan‐Piegza, Joanna; Piegza, Michał; Kancelista, Anna; Kurzawa, Adam; Żuk, Iwona; Kopeć, Wiesław

doi:10.1007/s12649-016-9603-4

Cited by 22 publications

(6 citation statements)

References 42 publications

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“…Based on Łaba et al, the most abundant amino acids found from the pig bristle or hair hydrolysates were leucine and cysteine. 14 Proline is also an amino acid found in the hair. 15 The trypsin-digested keratin hydrolysate sample (Supporting Information S1) also confirmed that the sample is peptides with molecular weight ranging from 800 to 2600 Da.…”

Section: Resultsmentioning

confidence: 99%

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Effect of Surfactant–Keratin Hydrolysate Interactions on the Hydration Properties of a Stratum Corneum Substitute

2020

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A novel stratum corneum substitute (SCS) has been developed, and the fundamental mechanism of the dehydration process has been studied using the SCS. After washing with cleansers which contain surfactants, our skin “feels” dehydrated (or hydrated). Although many studies have focused on the effect of surfactants on the regulation of the water loss by the lipid bilayers in the stratum corneum (SC) for a long timescale or at equilibrium, only few studies have focused on the acute effect of the surfactant interaction on dehydration. In addition, the interaction between the surfactant and keratin has been often underappreciated compared to lipid bilayers although keratin is the major nonaqueous component of the SC. Here, we have developed novel SCS models, nonkeratinized (lipid only) and keratinized, to study the effect of keratin hydrolysates on the dehydration rate. We have confirmed that the lipid organizational structure of the SCS was similar to that of the human SC using X-ray scattering. We have revealed that keratin hydrolysates play a significant role in the dehydration rate, accelerating the rate for the short term. We have also demonstrated that the effect of surfactants on dehydration is more pronounced for keratinized samples than that for the nonkeratinized sample. However, the dehydration rate for the nonkeratinized SCS with the surfactant became faster than the that for the keratinized SCS after the 20 min evaporation process, suggesting that the water binding sites of keratin hydrolysates slowed down evaporation, while the surfactant interacting with the lipids accelerated the water loss. Lastly, the study demonstrated that the SCS model can be a great platform to test macroscopic properties and analyze the underlying mechanism at the molecular level for various chemicals.

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Section: Resultsmentioning

confidence: 99%

“…Cysteine (C), glycine (G), proline (P), isoleucine (I), or leucine (L) were identified. Based on Łaba et al, the most abundant amino acids found from the pig bristle or hair hydrolysates were leucine and cysteine . Proline is also an amino acid found in the hair .…”

Section: Results and Discussionmentioning

confidence: 99%

Effect of Surfactant–Keratin Hydrolysate Interactions on the Hydration Properties of a Stratum Corneum Substitute

2020

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“…Proteolytic bacilli, which belong to the most frequently characterized keratinolytic bacteria, typically produce a number of activity bands, e.g. 7 in the case of B. subtilis 1271 (Mazotto et al 2011 ) or 7 bands in cultures of B. cereus PCM 2849 (Łaba et al 2017 ), both grown in feather-containing media. In contrast, keratinolytic cocci produced fewer extracellular proteases, i.e.…”

Section: Discussionmentioning

confidence: 99%

New keratinolytic bacteria in valorization of chicken feather waste

et al. 2018

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There is an increasing demand for cost-effective and ecologically-friendly methods for valorization of poultry feather waste, in which keratinolytic bacteria present a great potential. Feather-degrading bacteria were isolated from living poultry and a single strain, identified as Kocuria rhizophila p3-3, exhibited significant keratinolytic properties. The bacterial strain effectively degraded up to 52% of chicken feathers during 4 days of culture at 25 °C. Zymographic analysis revealed the presence of two dominating proteolytic enzymes in the culture fluid. Culture conditions were optimized in order to maximize the liberation of soluble proteins and free amino acids. A two-step procedure was used, comprising a Plackett–Burman screening design, followed by a Box–Behnken design. Concentration of feather substrate, MgSO4 and KH2PO4 were the most influential parameters for the accumulation of soluble proteins in culture K. rhizophila p3-3, while feathers and MgSO4 also affected the concentration of amino acids. The resultant raw hydrolysate supernatant, prior to and after additional treatments, was rich in phenylalanine, histidine, arginine and aspartic acid. Additionally the hydrolysate exhibited radical-scavenging activity and ferric reducing power.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0538-y) contains supplementary material, which is available to authorized users.

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“…Other treatments were described in individual studies, e.g. thermo‐chemical treatment (121°C, 20 min, 1 g bristle per 100 cm 3 sodium sulfite solution) (Łaba et al., 2016 ), or washing and degreasing with a methanol‐chloroform solution (Laba and Rodziewicz, 2014 ), among others. The main purpose of these treatments is the extraction of pure keratin and its subsequent conversion into smaller protein molecules of higher nutritional or industrial value through enzymatic, chemical or microbial based techniques.…”

Section: Assessmentmentioning

confidence: 99%

Inactivation of indicator microorganisms and biological hazards by standard and/or alternative processing methods in Category 2 and 3 animal by‐products and derived products to be used as organic fertilisers and/or soil improvers

Koutsoumanis¹,

Allende²,

Bolton³

et al. 2021

EFS2

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The European Commission requested EFSA to assess if different thermal processes achieve a 5 log 10 reduction in Enterococcus faecalis or Salmonella Senftenberg (775W) and (if relevant) a 3 log 10 reduction in thermoresistant viruses (e.g. Parvovirus ) as well as if different chemical processes achieve a 3 log 10 reduction of eggs of Ascaris sp., in eight groups of Category 2 and 3 derived products and animal by‐products (ABP). These included (1) ash derived from incineration, co‐incineration and combustion; (2) glycerine derived from the production of biodiesel and renewable fuels; (3) other materials derived from the production of biodiesel and renewable fuels; (4) hides and skins; (5) wool and hair; (6) feathers and down; (7) pig bristles; and (8) horns, horn products, hooves and hoof products. Data on the presence of viral hazards and on thermal and chemical inactivation of the targeted indicator microorganisms and biological hazards under relevant processing conditions were extracted via extensive literature searches. The evidence was assessed via expert knowledge elicitation. The certainty that the required log 10 reductions in the most resistant indicator microorganisms or biological hazards will be achieved for each of the eight groups of materials mentioned above by the thermal and/or chemical processes was (1) 99–100% for the two processes assessed; (2) 98–100% in Category 2 ABP, at least 90–99% in Category 3 ABP; (3) 90–99% in Category 2 ABP; at least 66–90% in Category 3 ABP; (4) 10–66% and 33–66%; (5) 1–33% and 10–50%; (6) 66–90%; (7) 33–66% and 50–95%; (8) 66–95%, respectively. Data generation on the occurrence and reduction of biological hazards by thermal and/or chemical methods in these materials and on the characterisation of the usage pathways of ABP as organic fertilisers/soil improvers is recommended.

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Enzymatic Degradation of Pretreated Pig Bristles with Crude Keratinase of Bacillus cereus PCM 2849

Cited by 22 publications

References 42 publications

Effect of Surfactant–Keratin Hydrolysate Interactions on the Hydration Properties of a Stratum Corneum Substitute

Effect of Surfactant–Keratin Hydrolysate Interactions on the Hydration Properties of a Stratum Corneum Substitute

New keratinolytic bacteria in valorization of chicken feather waste

Inactivation of indicator microorganisms and biological hazards by standard and/or alternative processing methods in Category 2 and 3 animal by‐products and derived products to be used as organic fertilisers and/or soil improvers

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