Enzymatic degradation of β-chitin: susceptibility and the influence of deacetylation

The use of lysozyme to prepare biologically active chitooligomers

“…On the other hand, susceptibility of chitin to degradation by lysozyme increasing with an increase in its deacetylation degree (DA). The maximum degradation was reached at about 50% deacetylation of chitosan; then, a continuous decrease was observed until 97% DA of chitosan [37] .…”

“…Extensive studies have been devoted to their structure, catalytic mechanism, relationship between structure and activity, phylogeny, immunology and genetics [34] . It catalyses hydrolysis of β (1-4) glycosidic linkages between N-acetylmuramic acid and N-acetyl D-glucosamine in the cell walls of polysaccharides, including bacteria [35] and also chitosan [36][37][38][39][40] . Due to its lytic properties, lysozyme was applied as an antimicrobial substance.…”

Influence of the Chemical Structure and Physicochemical Properties of Chitin- and Chitosan-Based Materials on Their Biomedical Activity

“…For example, scaffold degradation should be compatible with the rate of new tissue formation or be adequate for the controlled release of bioactive molecules. Degradation of chitosan has been shown to increase as the degree of deacetylation decreases (Hirano et al, 1989;Sashiwa et al, 1990;Kurita et al, 2000). Kofuji et al investigated the enzymatic digestion of various chitosans by observing changes in the viscosity of a chitosan solution in the presence of lysozyme (Kofuji et al, 2005).…”

“…Finally, it should also be mentioned that the lability of chitin towards lysozyme also depends on its crystalline form. Kurita et al showed that -chitin was degraded much more readily than -chitin owing to the weak intermolecular forces in the former (Kurita et al, 2000). Biocompatibility This property depends on many parameters, although DA seems to be one of the most important.…”

Desenvolvimento e análise de bandagem de bioestimulação dentino/pulpar (BBio)

“…Esses polímeros têm atraído o interesse de áreas da saúde como a farmacêutica, médica e odontológica por suas características de biodegradabilidade, biocompatibilidade e atoxidade (JAYAKUMAR et al, 2007;KUMAR, 2000;KURITA et al, 2000). A quitosana pode ser moldada em várias formas como hidrogéis, esponjas, membranas, nanofibras, micropartículas, apresentando também capacidade para formar fios e filmes.…”