Enzymatic determination of stevioside in Stevia rebaudiana

Mizukami, Hajime; Shiiba, K.; Ohashi, Hiromu

doi:10.1016/0031-9422(82)83016-1

Cited by 31 publications

(21 citation statements)

References 7 publications

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“…The stevioside and rebaudioside A contents of leaves and stems are within those reported in literature (Mizukami et al, 1982;Geuns, 2004;Hashimoto and Moriyasu, 1978). There was evidence from this study, of a preferential deposition of stevioside and rebaudioside A in the leaves.…”

Section: Discussionsupporting

confidence: 68%

Potential use of stevia rebaudiana in animal feeds

et al. 2011

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SHORT NOTE ADDITIONAL KEYWORDSNutritional value. Poultry feed. PALABRAS CLAVE ADICIONALESValor nutritivo. Alimento aves. SUMMARYThe nutritional profile of the leaves and stem of Stevia rebaudiana, and their potential utilization in an animal model were studied. Stevia leaves and stem had 16% and 6.7% crude protein and were low in fat content (2.6 and 1.1%) respectively. The fatty acid profile of fat showed a preponderance of unsaturated fatty acids (65.8% and 71.4% for leaves and stem respectively). Linolenic acid was the most abundant fatty acid in stevia leaf oil (36%) whereas linoleic acid was the highest in stems (38%). The crude fiber contents on dry fat free basis were 6.8% and 45.4% for leaves and stem respectively. The K content of stevia leaves and stem were comparable; Ca, Mg, Fe, Cu, Zn and Mn were higher in leaves and the opposite was true for Na. The tests with broiler chickens showed that apparent, nitrogen corrected, and true metabolisable energy values for leaves were 2113, 2098 and 2223 kcal/kg and for stems 1573, 1554 and 1675 kcal/kg respectively. Retention of the protein from the leaves and stems by chickens was 63% and 65.7% respectively. Stevia leaves contain other nutritional attributes besides of the sweetening components.

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Section: Discussionsupporting

confidence: 68%

Potential use of stevia rebaudiana in animal feeds

et al. 2011

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“…The reactions were left for 7 days and experiments were carried out in duplicate: to a set of experiments toluene was added (25) and, to the second set, ethanol was added (21). Organic solvents were added to the reactions aiming at promoting solvatation of reaction intermediaries and to cause beneficial modifications in enzymes conformations.…”

Section: Rhizopus Arrhizus and Rhizopus Stolonifer) And The Yeast Sacmentioning

confidence: 99%

“…Enzymatic hydrolysis of stevioside (1) into steviol (2) was previously accomplished by using hesperidinase, an enzymatic complex able to break glycoside bonds (14) and also by using the fungus Aspergillus niger, a filamentous fungus known to synthesize hydrolytic enzymes such as α-L-ramnosidase and β-D-glucosidase (21). Other successful examples of glycoside hydrolysis by means of biological reagents are the hydrolysis of isoflavone glycosides by bacteria (6), with yields ranging from 25 to 80%.…”

Section: Rhizopus Arrhizus and Rhizopus Stolonifer) And The Yeast Sacmentioning

confidence: 99%

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Novel agents for enzymatic and fungal hydrolysis of stevioside

Milagre¹,

Martins²,

Takahashi³

2009

Braz. J. Microbiol.

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A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi (Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae) and enzymes (pancreatin and lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0, using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8% yield).

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“…Various methods have been described for the estimation of stevioside content in stevia leaves including enzymatic determination, near infrared reflectance spectroscopy (NIRS) and HPLC (Mizukami et al, 1982, Nishiyama et al Ahmed and Dobberstein developed an HPLC method for the determination of eight diterpene glycosides. Satisfactory resolution was achieved on two protein columns in series, after the plant material was extracted with chloroform prior to methanol extraction (Mizukami et al, 1982). Therefore, considering its scope and future need of planting material, the present study was undertaken to find out suitable concentration and combination of cytokinin (BAP) and auxins (NAA, 2, 4-D, Kn) for callus induction and development from leaf explants, to study the production of steviol glycosides in callus culture and to compare the amount of stevioside between induced callus and leaf tissue using HPLC.…”

Section: Introductionmentioning

confidence: 99%

Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

Mahmud

Akter

Jahan

et al. 2015

Bangladesh J. Sci. Ind. Res.

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A protocol was developed to produce large amount of callus in short a period of time from leaf explants of Stevia rebaudiana Bert. The highest amount of white callus was obtained on MS medium supplemented with 2.5 mg/l 2, 4-D and 0.5 mg/l BAP after 3 weeks of inoculating leaf segments. On the other hand, 0.5 mg/l BAP and 1.0 mg/l Kn exhibits poor performance towards callus formation while after using 1.0 mg/l Kn alone did not develop any callus. In this experiment, highest amount of green callus was obtained when MS medium supplemented with 2.5 mg/l NAA and 10% coconut water was used. An improved analytical method HPLC was applied to analyze stevioside extracted from the leaf and callus of Stevia rebaudiana. The stevioside in each sample were analyzed by comparing their retention times with those of the standards. The retention time (RT) of stevioside for leaves were found 14.96 and for callus 13.81 mins. The percentage of stevioside content from leaves and callus was 12.19% and 12.62% respectively Key words: Stevioside; Callus; Natural sweeteners; Growth regulators; Stevia rebaudiana; HPLC Available online at www.banglajol.info Bangladesh J. Sci. Ind. Res. 49(4), [199][200][201][202][203][204] 2014 *Corresponding author: E-mail: trishansu@gmail.com (Mizukami et al., 1982, Nishiyama et al. Ahmed and Dobberstein developed an HPLC method for the determination of eight diterpene glycosides. Satisfactory resolution was achieved on two protein columns in series, after the plant material was extracted with chloroform prior to methanol extraction (Mizukami et al., 1982). Therefore, considering its scope and future need of planting material, the present study was undertaken to find out suitable concentration and combination of cytokinin (BAP) and auxins (NAA, 2, 4-D, Kn) for callus induction and development from leaf explants, to study the production of steviol glycosides in callus culture and to compare the amount of stevioside between induced callus and leaf tissue using HPLC. Materials and methodsThe experiment was conducted at Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka Bangladesh. The experiment was performed in two phases. In the first phase, leaf segments of Stevia rebaudiana, as explants were cultured for callus induction on MS medium (Murashige and Skoog, 1962) supplemented with auxins (2,4-D, NAA) and cytokinin (BAP, Kn) at different concentrations (0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/l ) and with 10% coconut water respectively. Regular visual observation was done up to five weeks to record the day of callus initiation and maturation. Then fresh weight of best calluses and fresh leaves were recorded.In the second phase of this study stevioside content was determined in the callus and leaf from which the callus was developed by HPLC method. The HPLC system was a SHIMADJU SPD-M20A chromatograph equipped with a binary pump and UV detection was at 219 am. The analytical column was RP-C18 (250 mm X 4.6 nm). Separation was performed with a water and methano...

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Enzymatic determination of stevioside in Stevia rebaudiana

Cited by 31 publications

References 7 publications

Potential use of stevia rebaudiana in animal feeds

Potential use of stevia rebaudiana in animal feeds

Novel agents for enzymatic and fungal hydrolysis of stevioside

Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

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