Enzymatic Effects on Cell Rupture in Plant Protein Recovery

Mudgett, R. E.; Rufner, Robyn; Bajracharya, Rupak; Kim, K.; Rajagopalan, K.

doi:10.1111/j.1745-4514.1978.tb00612.x

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…32%). These low protein yields were attributed to the pH used for extraction (pH 4-5), which coincided with the minimum solubilities of glycinin and β-conglycinin (1), and confirmed the results obtained in previous studies (2,5). Our results revealed that pH adjustment to a value of high protein solubility (pH 7), followed by stirring for 30 min, was insufficient to totally resolubilize the precipitated proteins.…”

Section: Resultssupporting

confidence: 90%

“…The consecutive addition of 5% IndiAge Super L to a slurry first extracted with 10% Multifect pectinase for 3 h at pH 4 did not enhance the protein extraction compared with extraction without IndiAge Super L. The protein extraction yield and reducing sugar production were the same as those achieved with Multifect pectinase alone (results not shown). The synergistic effect of the cellulase-pectinase mixture on protein extraction obtained with alfalfa leaves was not observed in our experimental conditions with soybeans (5). Even if some of the enzyme cocktails increased the reducing sugar yield, none of them significantly increased protein extraction compared with single enzymes.…”

Section: Resultscontrasting

confidence: 58%

“…Solubility profiles of the control SPI were typical U-shaped curves, with the lowest solubility around the protein isoelectric point (pH [4][5]. No significant changes in solubility behavior were observed for the enzyme-treated samples (results not shown).…”

Section: Resultsmentioning

confidence: 90%

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Functionality of soy protein produced by enzyme‐assisted extraction

Jung¹,

Lamsal²,

Stepien³

et al. 2006

J Americ Oil Chem Soc

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This study investigated the potential of enzymes to increase soy protein extractability without causing protein degradation. The aqueous extraction of protein was performed from defatted soy flakes on a laboratory-and pilot-plant scale. Yields of protein and reducing sugars were determined in the alkali extracts obtained with cellulases and pectinase, added alone or as cocktails. Using 5% (wt/g of protein) Multifect pectinase resulted in the best improvement of protein yields, which were 50 and 17% greater than the controls in laboratory-and pilot-plant-scale trials, respectively. This enhanced protein extraction was accompanied by an increased reducing sugar content in the aqueous extract compared with the control. Under the conditions tested, no enzyme cocktail markedly increased the protein yield compared with the use of single enzymes. The solubility curve for Multifect pectinase-treated soy protein isolate (SPI) was typical of SPI at pH 2-10. Its foam stability significantly improved, but the emulsification properties declined. Multifect pectinase markedly reduced the viscosity of SPI. SDS-PAGE showed that the α′ and α subunits of β-conglycinin were modified, and glycoprotein staining showed that these modifications were probably due to a protease secondary activity in the pectinase preparation. One cellulase and one pectinase were identified as effective in modifying the protein and reducing sugar extractability from the defatted soy flakes.Paper no. J11184 in JAOCS 83, 71-78 (January 2006).

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Section: Resultssupporting

confidence: 90%

Section: Resultscontrasting

confidence: 58%

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Functionality of soy protein produced by enzyme‐assisted extraction

Jung¹,

Lamsal²,

Stepien³

et al. 2006

J Americ Oil Chem Soc

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“…Enzymatic methods may be used to increase the yield at neutral pH by liberating more protein from the polysaccharide matrix. Methods describing the application of enzymes, particularly carbohydrases, to improve the extractability of protein from plant sources have been presented (Ghose and Haldar 1969 ;Mudgett et al 1978 ;Grossman et al 1980).…”

Section: Introductionmentioning

confidence: 99%

Application of Carbohydrases in Extracting Protein from Rice Bran

Ansharullah

Hourigan

Chesterman

1997

J. Sci. Food Agric.

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Enzymatic pretreatments of rice bran, using commercial carbohydrases to improve nitrogen extractability, were investigated. The pretreatment used a response surface method which included the variations in concentration of Viscozyme L (0–120 FBG unit) and Celluclast 1.5L (0–360 NC unit), time of incubation (1–5 h) and pH (3·8–5·4). The experiments were conducted at two different temperatures, 40 and 50°C. At 40°C, the maximum nitrogen extraction predicted by the model was obtained when the Viscozyme–Celluclast mixture at pH 3·8 with incubation time of 5 h was applied, giving an extracted N of 51%. At the process temperature of 50°C, the model predicted that the use of Viscozyme alone, under similar conditions, would have a most significant effect in increasing the efficacy of the nitrogen extraction, producing a 57% extracted N. This enzymatic pretreatment method improved the nitrogen extractability of rice bran at the neutral pH. © 1997 SCI.

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“…[17] This allows rupturing of the plant cells. In addition, use of solvent, which is either produced in-situ (like ethanol and vinegar)[2728] or added separately allows compounds to diffuse from cells into the medium.…”

Section: Methodsmentioning

confidence: 99%

Proposed correlation of modern processing principles for Ayurvedic herbal drug manufacturing: A systematic review

Jain

Venkatasubramanian

2014

Ancient Sci Life

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Quality Ayurvedic herbal medicines are potential, low-cost solutions for addressing contemporary healthcare needs of both Indian and global community. Correlating Ayurvedic herbal preparations with modern processing principles (MPPs) can help develop new and use appropriate technology for scaling up production of the medicines, which is necessary to meet the growing demand. Understanding the fundamental Ayurvedic principles behind formulation and processing is also important for improving the dosage forms. Even though Ayurvedic industry has adopted technologies from food, chemical and pharmaceutical industries, there is no systematic study to correlate the traditional and modern processing methods. This study is an attempt to provide a possible correlation between the Ayurvedic processing methods and MPPs. A systematic literature review was performed to identify the Ayurvedic processing methods by collecting information from English editions of classical Ayurveda texts on medicine preparation methods. Correlation between traditional and MPPs was done based on the techniques used in Ayurvedic drug processing. It was observed that in Ayurvedic medicine preparations there were two major types of processes, namely extraction, and separation. Extraction uses membrane rupturing and solute diffusion principles, while separation uses volatility, adsorption, and size-exclusion principles. The study provides systematic documentation of methods used in Ayurveda for herbal drug preparation along with its interpretation in terms of MPPs. This is the first step which can enable improving or replacing traditional techniques. New technologies or use of existing technologies can be used to improve the dosage forms and scaling up while maintaining the Ayurvedic principles similar to traditional techniques.

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Enzymatic Effects on Cell Rupture in Plant Protein Recovery

Cited by 11 publications

References 32 publications

Functionality of soy protein produced by enzyme‐assisted extraction

Functionality of soy protein produced by enzyme‐assisted extraction

Application of Carbohydrases in Extracting Protein from Rice Bran

Proposed correlation of modern processing principles for Ayurvedic herbal drug manufacturing: A systematic review

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