Enzymatic Factors in Renal Tubular Secretion of Phenol Red

Beyer, Karl H.; Painter, R. H.; Wiebelhaus, Virgil D.

doi:10.1152/ajplegacy.1950.161.2.259

Cited by 42 publications

(9 citation statements)

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“…S/M ratio decreases in the presence of iodoacetate and fluoroacetate, as well as the above mentioned substances. As stated previously, Shideman & Rene (20) have been indicated that succinic oxidase system affects the accumulation of PAH in kidney slice, and Beyer et al (4) suggested that succinic dehydrogenase has an intimate relation to excre tion of phenol red based on the confirmation of the inhibitory actions of Hg ion, quinone, and phenylhydrazine, etc.…”

Section: ) the Stimulatory Effectmentioning

confidence: 82%

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Studies on Renal Tubular Transport

Koishi

1959

Japanese Journal of Pharmacology

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Section: ) the Stimulatory Effectmentioning

confidence: 82%

“…Beyer et al (3,4) have studied the effect of inhibitors using the technique of Forster and concluded that phenol red transport is performed by excretion through tubulus cells and it was closely related with energy-rich phosphate bonds.…”

Section: Introductionmentioning

confidence: 99%

Studies on Renal Tubular Transport

Koishi

1959

Japanese Journal of Pharmacology

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“…S s5 uptake has been shown, in the present report, to differ from these processes in various details, including lack of inhibition by diodrast and other secreted compounds, and a differing pattern of pH and anion sensitivity. Phlorhizin has an inhibitory effect on the uptake of PAH and phenol red as well as potassium (4,5,12), in contrast to its enhancing action on tissue relative sulfate volume. Acetate, which increases PAH accumulation specifically, depressed S s uptake as did other metabolic intermediates tested.…”

Section: Discussionmentioning

confidence: 99%

Further Observations on Uptake of S35-Labelled Sulfate by Renal Tissue in Vitro

Deyrup

1956

The Journal of General Physiology

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Additional studies have been made of the accumulation of S35 by renal cortical tissue incubated in media containing radiosulfate. This process was found to occur in several mammalian species in addition to the rat, but was not observed as a significant occurrence in three species of lower vertebrates. In the case of rat renal tissue, S35 uptake was found to be sensitive to the pH and osmolar concentration of the medium. The character of the anions present in conjunction with K+ affected it as well. Various factors known to be related to in vitro accumulative processes, as well as to renal sulfate reabsorption by the intact dog, were tested on rat kidney cortex to assess the effect on radiosulfate uptake. In general, all substances tested (amino acids, metabolic intermediates, ATP, metabolic inhibitors, competitive inhibitors for PAH accumulation in vitro) were found to lessen S35 uptake, or to be without effect upon it. The one striking exception was phlorhizin, which enhanced markedly S35 uptake in vitro, as it does sulfate reabsorption in vivo. Some implications of these findings have been discussed.

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“…The wide range of effects of metabolites, poisons, etc. which has been used to characterize the PAH and phenol red uptake processes in vitro (26,27 and others) has not been established as yet for the process of sulfate accumulation. In essence, the interest of these methods for studying renal accumulative processes in vitro lies primarily in the possibility of manipulating and defining relatively exactly the conditions under which tubular transport of specific compounds may occur (5).…”

Section: Discussionmentioning

confidence: 99%

Accumulation of Sulfate Labelled With S35 by Rat Tissue in Vitro

Deyrup

Ussing

1955

The Journal of General Physiology

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Rat kidney slices incubated in vitro may show, in parallel with other shifts in electrolyte content, a striking capacity for accumulating sulfate ion (sulfate labelled with S35). The uptake is reversed or reduced by CN-, cooling to room temperature, and by interference with adequate oxygenation. Under the conditions of the experiment, the presence in the medium of sodium acetate and glucose as substrates was found to be without measurable effect on the accumulation. The extent of sulfate uptake is related to the ionic composition of the medium in which the tissue is incubated, for the uptake occurs optimally only in the presence of a K+ level of about 0.04 M, and is decreased as the concentration of Na+ rises. Likewise, when Ca++, Mg++, or choline is present in the medium, sulfate accumulation may be depressed. In addition to renal cortex, kidney medulla and liver showed capacity for sulfate accumulation, whereas no convincing evidence for significant uptake was obtained with strips of aorta, colon, or diaphragm.

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Enzymatic Factors in Renal Tubular Secretion of Phenol Red

Cited by 42 publications

References 0 publications

Studies on Renal Tubular Transport

Studies on Renal Tubular Transport

Further Observations on Uptake of S35-Labelled Sulfate by Renal Tissue in Vitro

Accumulation of Sulfate Labelled With S35 by Rat Tissue in Vitro

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