Enzymatic formation of prostaglandin D2, E2, and F2α in the parasitic protozoan Trypanosoma brucei

Kubata, Bruno Kilunga; Duszenko, Michael; Kabututu, Zakayi; Rawer, Marc; Szallies, Alexander; Inui, Takashi; Urade, Yoshihiro; Hayaishi, Osamu

doi:10.1016/s0531-5131(02)00274-1

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…[7][8][9] Following up reports about an increase of prostaglandin (PG) levels in the serum and cerebrospinal fluid of sleeping sickness patients, 10 we have shown that trypanosomes produce PGD 2 , PGE 2 and PGF 2a from arachidonic acid. 11,12 These PGs led to a broad variety of different physiological effects in higher eukaryotes 13 and their accumulation in serum coincides remarkably with symptoms observed during trypanosomiasis, such as fever, pain, immunosuppression, dysregulation of sleep/wake cycles and others. So far, it is not clear why protozoa produce PGs, but it is tempting to speculate that these parasites may have adopted the formation of PGs to modify host reactions for their own benefit.…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin D2 induces programmed cell death in Trypanosoma brucei bloodstream form

et al. 2005

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African trypanosomes produce some prostanoids, especially PGD 2 , PGE 2 and PGF 2a (Kubata et al. 2000, J. Exp. Med. 192: 1327-1338, probably to interfere with the host's physiological response. However, addition of prostaglandin D 2 (but not PGE 2 or PGF 2a ) to cultured bloodstream form trypanosomes led also to a significant inhibition of cell growth. Based on morphological alterations and specific staining methods using vital dyes, necrosis and autophagy were excluded. Here, we report that in bloodstream form trypanosomes PGD 2 induces an apoptosis-like programmed cell death, which includes maintenance of plasma membrane integrity, phosphatidylserine exposure, loss of mitochondrial membrane potential, nuclear chromatin condensation and DNA degradation. The use of caspase inhibitors cannot prevent the cell death, indicating that the process is caspase-independent. Based on these results, we suggest that PGD 2 -induced programmed cell death is part of the population density regulation as observed in infected animals.

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Section: Introductionmentioning

confidence: 99%

Prostaglandin D2 induces programmed cell death in Trypanosoma brucei bloodstream form

et al. 2005

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“…22 In the bloodstream form of T. brucei, an apoptosis-like programmed cell death (PCD) is induced by prostaglandin D 2 (PGD 2 ). 23 PGD 2 is the major prostanoid produced and secreted by the stumpy bloodstream form of T. brucei, 24 which was shown to be more sensitive to this compound than the slender bloodstream form. 23 In this sense, the physiological function of PGD 2 in trypanosomes can be related to the population density regulation by PCD induction.…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin-induced programmed cell death in Trypanosoma brucei involves oxidative stress

et al. 2006

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Recently, we reported the induction of a programmed cell death (PCD) in bloodstream forms of Trypanosoma brucei by prostaglandin D 2 (PGD 2 ). As this prostanoid is readily metabolized in the presence of albumin, we were prompted to investigate if PGD 2 metabolites rather than PGD 2 itself are responsible for the observed PCD. In fact, J series metabolites, especially PGJ 2 and D 12 PGJ 2 , were able to induce PCD more efficiently than PGD 2 . However, the stable PGD 2 analog 17phenyl-trinor-PGD 2 led to the same phenotype as the natural PGD 2 , indicating that the latter induces PCD as well. Interestingly, the intracellular reactive oxygen species (ROS) level increased significantly under J series metabolites treatment and, incubation with N-acetyl-L-cysteine or glutathione reduced ROS production and cell death significantly. We conclude that PGJ 2 and D 12 PGJ 2 formation within the serum represents a mechanism to amplify PGD 2 -induced PCD in trypanosomes via ROS production.

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“…PGH2 is the common key players in determining the reproductive efficiency precursor of all PGs generated by specific terminal in farm animal species. The ISG15/17 gene shares limited Till now several PGFS have been identified, from cow (~30%) amino acid sequence identity with a tandem [45,46], human [47], sheep [48], Trypanosoma brucei ubiquitin repeat [24,26] Thus, both these proteins are [49] and porcine endometrium [50]. PGF2α is a bioactive lipid belonging to the stimulated gene- 17 (ISG17) in bovine [24] and ovine eicosanoid family.…”

Section: Ubiquitin Cross Reative Protein (Ucrp)mentioning

confidence: 99%

Role of candidate genes in regulation of embryonic survival and maternal recognition of pregnancy in farm animals

Kumar¹,

Ramteke²,

Nath³

et al. 2013

Vet World

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Successful growth and development of the post-hatching blastocyst and pregnancy establishment are results of the interaction between a competent embryo and a receptive uterine environment. There are certain marker gene transcripts identified which contribute and regulate the bidirectional channel of communication during the pregnancy period in farm animals. The changes in temporal gene expression in the endometrium are similar in pregnant and cyclic animals up to the time of maternal recognition of pregnancy (MRP) when conceptus derived factors affect expression of a large number of genes in pregnant animals. In this review we have discussed a set of candidate genes expressed or induced for MRP. There are certain genes playing important role in MRP in farm animals, of which interferon-tau (IFNT), Ubiquitin Cross Reative Protein (UCRP), Ghrelin, Aldoketoreductase-1B5 (AKR1B5), SERPINA14 are having esteemed role in farm animals. These genes appear to have role in successful establishment of pregnancy and expression of the cascade of signalling molecules. However, IFNT was recognized as one of the earliest expressing gene specifically required for maintenance of pregnancy. [Vet World 2013; 6(5.000): 280-284

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Enzymatic formation of prostaglandin D2, E2, and F2α in the parasitic protozoan Trypanosoma brucei

Cited by 4 publications

References 14 publications

Prostaglandin D2 induces programmed cell death in Trypanosoma brucei bloodstream form

Prostaglandin D2 induces programmed cell death in Trypanosoma brucei bloodstream form

Prostaglandin-induced programmed cell death in Trypanosoma brucei involves oxidative stress

Role of candidate genes in regulation of embryonic survival and maternal recognition of pregnancy in farm animals

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