Enzymatic Hydrolysis of Ammonia-Treated Sugar Beet Pulp

Foster, Brian L.; Dale, Bruce E.; Doran‐Peterson, Joy

doi:10.1007/978-1-4612-0217-2_23

Cited by 8 publications

(10 citation statements)

References 23 publications

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“…The moderate temperatures and pH values in AFEX process minimize formation of sugar degradation products while giving high monomeric sugar yields. AFEX pretreatment gives close to theoretical glucose yields at relatively low enzyme loadings (<5 FPU per gram of biomass or 20 FPU/g cellulose) (Dale, 1986;Dale and Moreira, 1982;Holtzapple et al, 1991;Dale et al, 1996;Moniruzzaman et al, 1997;Foster et al, 2001). Increases in glucan conversion by about six fold and xylan conversion by almost 23 fold with AFEX pretreatment, compared no-pretreatment have been reported (Teymouri et al, 2005).…”

Section: Figure 3: the Concept Of Regional Biomass Processing Center mentioning

confidence: 82%

Technical and Financial Feasibility Analysis of Distributed Bioprocessing Using Regional Biomass Pre-Processing Centers

Carolan

Joshi²,

Dale³

2007

Journal of Agricultural &Amp; Food Industrial Organization

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Research indicates that large biorefineries capable of handling 5000-10000MT of biomass per day are necessary to achieve process economies. However, such large biorefineries also entail increased costs of biomass transportation and storage, high transaction costs of contracting with a large number of farmers for biomass supply, potential market power issues, and local environmental impacts. We propose a network of regional biomass preprocessing centers (RBPC) that form an extended biomass supply chain feeding into a biorefinery, as a way to address these issues. The RBPC, in its mature form, is conceptualized as a flexible processing facility capable of pre-treating and converting biomass into appropriate feedstocks for a variety of final products such as fuels, chemicals, electricity, and animal feeds. We evaluate the technical and financial feasibility of a simple RBPC that uses ammonia fiber expansion pretreatment process and produces animal feed along with biorefinery feedstock.

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Section: Figure 3: the Concept Of Regional Biomass Processing Center mentioning

confidence: 82%

Technical and Financial Feasibility Analysis of Distributed Bioprocessing Using Regional Biomass Pre-Processing Centers

Carolan

Joshi²,

Dale³

2007

Journal of Agricultural &Amp; Food Industrial Organization

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“…Samples from cellulose or SBP grown cultures showed a high H 2 O 2 formation rate and unexpectedly this was reduced by addition of CDH. Currently we have no explanation of this effect, but we assume that the addition of N. crassa CDH inhibits Standard (M), and culture supernatants of S. rolfsii (1), T. multicolor (2) and N. crassa (3); CMC-zymogram lanes show culture supernatants of S. rolfsii (4), T. multicolor (5), and N. crassa (6).…”

Section: Extracellular Enzymatic Activities Of Fungal Cultures Grown mentioning

confidence: 99%

“…234.8 μL g -1 [8] or 10.66 mL kg -1 [34]. Also, Foster et al [6] stated that the "level of commercially available fungal enzymes required to increase ethanol yields [from SBP] to distillable levels may be too high" due to the high cost of enzymes. Ammonium pre-treatment was suggested to increase yields, but actually the yield of hydrolysed carbohydrates was higher in untreated samples.…”

Section: Biotechnology Journalmentioning

confidence: 99%

“…The efficiency of enzymatic SBP hydrolysis is low and restricted by the complex composite structure, which hinders access of enzymes to their specific substrates. Current strategies designed to achieve fast and complete hydrolysis involve thermal or acid/base pretreatments in combination with high cellulase activities [6][7][8]. In previous studies hydrolyzation of SBP into soluble sugars was also accomplished by treatment with pectinase and cellulases [9,10].…”

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Fungal secretomes enhance sugar beet pulp hydrolysis

Kracher

Oros

Yao

et al. 2014

Biotechnology Journal

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The recalcitrance of lignocellulose makes enzymatic hydrolysis of plant biomass for the production of second generation biofuels a major challenge. This work investigates an efficient and economic approach for the enzymatic hydrolysis of sugar beet pulp (SBP), which is a difficult to degrade, hemicellulose-rich by-product of the table sugar industry. Three fungal strains were grown on different substrates and the production of various extracellular hydrolytic and oxidative enzymes involved in pectin, hemicellulose, and cellulose breakdown were monitored. In a second step, the ability of the culture supernatants to hydrolyze thermally pretreated SBP was tested in batch experiments. The supernatant of Sclerotium rolfsii, a soil-borne facultative plant pathogen, was found to have the highest hydrolytic activity on SBP and was selected for further hydrolyzation experiments. A low enzyme load of 0.2 mg g–1 protein from the culture supernatant was sufficient to hydrolyze a large fraction of the pectin and hemicelluloses present in SBP. The addition of Trichoderma reesei cellulase (1–17.5 mg g–1 SBP) resulted in almost complete hydrolyzation of cellulose. It was found that the combination of pectinolytic, hemicellulolytic, and cellulolytic activities works synergistically on the complex SBP composite, and a combination of these hydrolytic enzymes is required to achieve a high degree of enzymatic SBP hydrolysis with a low enzyme load.

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“…It employs various alkaline reagents including sodium hydroxide [1,2], calcium hydroxide [3,4], potassium hydroxide [5], aqueous ammonia [6,7], ammonia hydroxide [8], and sodium hydroxide in combination with hydrogen peroxide [9,10]. Mechanistically, alkali is believed to cleave hydrolysable linkages in lignin and glycosidic bonds of polysaccharides, which causes a reduction in the degree of polymerization and crystallinity, swelling of the fibers, as well as disruption of the lignin structure [11].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Corn Stover Pretreated with Liquid Hot Water and Fed-Batch Semi-Simultaneous Saccharifi cation and Fermentation for Bioethanol Production

Gikonyo¹

2015

Fuel Production From Non-Food Biomass

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Background: Previous research on alkaline pretreatment has mainly focused on optimization of the process parameters to improve substrate digestibility. To achieve satisfactory sugar yield, extremely high chemical loading and enzyme dosages were typically used. Relatively little attention has been paid to reduction of chemical consumption and process waste management, which has proven to be an indispensable component of the biorefineries. To indicate alkali strength, both alkali concentration in pretreatment solution (g alkali/g pretreatment liquor or g alkali/L pretreatment liquor) and alkali loading based on biomass solids (g alkali/g dry biomass) have been widely used. The dual approaches make it difficult to compare the chemical consumption in different process scenarios while evaluating the cost effectiveness of this pretreatment technology. The current work addresses these issues through pretreatment of corn stover at various combinations of pretreatment conditions. Enzymatic hydrolysis with different enzyme blends was subsequently performed to identify the effects of pretreatment parameters on substrate digestibility as well as process operational and capital costs. Results: The results showed that sodium hydroxide loading is the most dominant variable for enzymatic digestibility. To reach 70% glucan conversion while avoiding extensive degradation of hemicellulose, approximately 0.08 g NaOH/g corn stover was required. It was also concluded that alkali loading based on total solids (g NaOH/g dry biomass) governs the pretreatment efficiency. Supplementing cellulase with accessory enzymes such as α-arabinofuranosidase and β-xylosidase significantly improved the conversion of the hemicellulose by 6-17%.

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Enzymatic Hydrolysis of Ammonia-Treated Sugar Beet Pulp

Cited by 8 publications

References 23 publications

Technical and Financial Feasibility Analysis of Distributed Bioprocessing Using Regional Biomass Pre-Processing Centers

Technical and Financial Feasibility Analysis of Distributed Bioprocessing Using Regional Biomass Pre-Processing Centers

Fungal secretomes enhance sugar beet pulp hydrolysis

Characteristics of Corn Stover Pretreated with Liquid Hot Water and Fed-Batch Semi-Simultaneous Saccharifi cation and Fermentation for Bioethanol Production

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