Enzymatic hydrolysis of lignocellulosic biomass using native cellulase produced by Aspergillus niger ITV02 under liquid state fermentation

Abstract

The objective of this work was to evaluate the biochemical characteristics of an enzymatic extract obtained from autochthonous fungus Aspergillus niger ITV02 and its application in the enzymatic hydrolysis of wheat straw and corn stubble pretreated by steam explosion. The enzymatic extract was obtained by submerged fermentation using delignified sweet sorghum bagasse as a carbon source. The results obtained showed that the enzymatic extract had β-glucosidase and endoglucanase activities. The effects of pH and temperature on cellulase activity were evaluated and its thermostability was determined. The optimal parameters of the β-glucosidase and endoglucanase activities obtained were pH 5 and 70 °C. The enzymatic extract of A. niger ITV02 was used to hydrolyze wheat straw and corn stubble, and the hydrolysis yields were compared with those obtained by a commercial cellulase (Celluclast 1.5L NS 50013) and CellicCTec3. The results showed that with the use the mixture of Celluclast 1.5L-A. niger ITV02 and CellicCTec3-A. niger ITV02 in the hydrolysis, conversions of 86.36% and 67.8% were obtained, respectively. Glucose production for the mixture extract increased 2.15 times more than when the enzyme was used independently alone. The present work shows that A. niger ITV02 has a potential as an enzyme producer for lignocellulosic hydrolysis.

Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

“…Fonte de referência não encontrada.). Previous studies of β-glucosidase characterization produced by A. niger monocultures have reported maximum activities at pH ranges from 3.5 to 5.5, corroborating the present results (Rodrigues et al, 2017;Infanzón-Rodríguez et al, 2021). For example, Dos Santos et al (2015) observed optimal activity at pH 3.5 for β-glucosidase from A. niger SCBM3, while Rodrigues et al (2017) obtained optimum pH of 4.5 for β-glucosidase from A. niger SCBM1.…”

Contribution of glycosidically bound compounds to aroma potential of jackfruit (Artocarpus heterophyllus lam)

“…Three replicates and a control (no enzyme added) were prepared. The enzymatic treatment was conducted at 50°C for 24 h with constant stirring according to optimal conditions 26 . The hydrolysate was cooled over ice, and the released aglycones were extracted with a separatory funnel using liquid–liquid extraction (LLE) five times with 20 mL of a mixture of n ‐pentane:diethyl ether (1:1, v/v) 13,21 .…”

“…The enzymatic treatment was conducted at 50°C for 24 h with constant stirring according to optimal conditions. 26 The hydrolysate was cooled over ice, and the released aglycones were extracted with a separatory funnel using liquid-liquid extraction (LLE) five times with 20 mL of a mixture of n-pentane:diethyl ether (1:1, v/v). 13,21 The extract was dried over anhydrous Na 2 SO 4 , concentrated, and stored in the same way as the extract from the free fraction mentioned above.…”