Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4-xylanase: Effect of pH, temperature, enzyme loading and reaction time

Abstract

Pineapple leaves; is one of lignocellulosic materials that composed of about 80% of carbohydrate containing lignin, cellulose and hemicellulose This waste still retains a considerable amount of soluble sugars that can be transformed into valuable fermentable sugars. This research was conducted to study the effect of different pH, temperature, enzyme loading and reaction time in order to identify the best working conditions to produce the highest yield of sugar. Prior to enzymatic hydrolysis, the pineapple leaves were subjected to hydrothermal pretreatment in order to obtain the hydrolysate liquid. Enzymatic hydrolysis was then carried out by using endo-1,4-xylanase and the reducing sugar was analyzed by using dinitrosalicylic (DNS) method. The result has shown that the highest reducing sugar concentration of 70.9 mg/L was obtained both at pH 5.5 and 50 °C. In addition, the best enzyme loading and reaction time were recorded at 0.5% (w/v) and 45 min that resulted 60.1 mg/L and 72. 0 mg/L respectively.

High Sugar Production from Hydrolysate of Pineapple Residues via Integrated Enzyme‐Membrane System

“…5 M NaOH and 5 M H 2 SO 4 were used to achieve the required pH. For the effect of temperature, three different temperatures, i.e., 40 °C, 50 °C, and 60 °C, were tested [20]. The best enzyme loading was identified by comparing three different enzyme loadings: 0.5 %, 1 %, and 2 % w/v [20,21].…”

“…For the effect of temperature, three different temperatures, i.e., 40 °C, 50 °C, and 60 °C, were tested [20]. The best enzyme loading was identified by comparing three different enzyme loadings: 0.5 %, 1 %, and 2 % w/v [20,21]. Lastly, the effect of reaction time was investigated at three distinct time intervals:, namely, 15, 30, and 45 min, for the hydrolysis reaction [20,21].…”

Kajian Pembuatan Bioetanol dari Limbah Kulit Nanas (Ananas comosus. L)

“…Pada penelitian dengan menggunakan enzim [13], hidrolisis menggunakan enzyme Trichoderma harzianum, selulosa, dan campuran Trichoderma harzianum dan selulosa dengan konsentrasi 2% didapatkan nilai gula reduksi tertingggi pada hidrolisis dengan menggunakan selulosa dan dari penelitiannya yang lain, hidrolisis dengan menggunakan enzim konsentrasi 2% Trichoderma harzianum menghasilkan gula reduksi lebih tinggi daripada tidak adanya hidrolisis [13]. Adapun tingginya konsentrasi enzim tidak meningkatkan jumlah gula reduksi pada hirolisis yang terjadi seperti pada penelitian Rosdee et al [20]. Hidrolisis yang dilakukan dengan enzim endo-1,4-xylanase dengan konsentrasi 0,5%; 1%; dan 2%, didapatkan nilai gula reduksi tertinggi pada konsentrasi 0,5%.…”

“…Hidrolisis yang dilakukan dengan enzim endo-1,4-xylanase dengan konsentrasi 0,5%; 1%; dan 2%, didapatkan nilai gula reduksi tertinggi pada konsentrasi 0,5%. Sehingga dapat dikatakan kenaikan konsentrasi enzim tidak dapat meningkatkan kinerja enzimatik, karena enzim yang berlebih tidak dapat mendepolimerisasi biomassa biosakarida [20].…”

“…Dalam fermentasi, pH digunakan dalam menciptakan kondisi lingkungan sebagai tempat tumbuh Saccharomyces cerevisiae dalam mengubah glukosa menjadi etanol. Ketidaksesuaian pH dengan lingkungan fermentasi dapat merugikan pertumbuhan ragi [20]. pH rendah dapat memperlambat proses fermentasi adapun pada pH lebih tinggi akan meningkatkan aktivitas fermentasi namun dapat menghasilkan produk samping yang berupa gliserin [21].…”