Enzymatic hydrolysis of okara protein concentrate by mixture of endo and exopeptidase

Pereira, Dafne Garcia; Justus, Ariana; Falcão, Heloisa Gabriel; Rocha, Thaís de Souza; Ida, Elza Iouko; Kurozawa, Louise Emy

doi:10.1111/jfpp.14134

Cited by 15 publications

(5 citation statements)

References 29 publications

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“…High salt concentrations in soil solution increases the osmotic stress of soil organisms because of the higher energy requirement to withdraw water from the soil or retain it in cells, the condition is known as "physiological drought" (Setia et al., 2013). These degraded soil conditions lead to reduction in microbial biomass (Mavi & Marschner, 2013), soil enzymatic activity (Pereira et al., 2019), and ultimately low soil CO 2 efflux (Baumann & Marschner, 2013). The inherent soil organic C of both subsoils were extremely low, less than 1% organic matter (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Soil carbon dioxide efflux from amended saline and thermally desorbed oil‐contaminated subsoil

Kruger

Sigdel

Gasch

et al. 2020

Agrosystems Geosci & Env

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Soil carbon (C) dynamics are often challenged by soil properties like high salt concentration and low organic matter content. To examine options for improving the quality of degraded soils, two pairs of soils, (a) non-saline and (b) saline surface soils from an agricultural field, and (a) non-contaminated subsoil and (b) thermal desorption-treated subsoil from an oil-contaminated site were collected. For the laboratory incubation, these four soils were treated with three amendments, (a) composted beef manure, (b) spent lime from sugarbeet (Beta vulgaris L.) processing, and (c) commercial mulch (Proganics), and soil CO 2 efflux was measured for three consecutive drying-rewetting (DW) cycles. Base efflux and the rate of change in base efflux were calculated from the slope and intercept of a linear fit model of CO 2 efflux over time (h), respectively for each DW cycle. Soil, amendment, and DW had significant effect on base efflux and the rate of change in base efflux during DW. Proganics had the highest base efflux of 29.0 and 24.0 μg CO 2-C m −2 d −1 for saline and thermal desorption-treated soils, respectively, and significantly (P < .05) higher than other amendments. Spent lime and compost also significantly (P < .05) increased base efflux over the control. Base efflux had a strong association with permanganate oxidizable carbon (POXC) for all soils, and a strong association with inorganic N for only saline and non-saline soils. Amendments increased microbial metabolism and have the potential to improve soil health and crop production for soils with severe limitations.

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Section: Discussionmentioning

confidence: 99%

Soil carbon dioxide efflux from amended saline and thermally desorbed oil‐contaminated subsoil

Kruger

Sigdel

Gasch

et al. 2020

Agrosystems Geosci & Env

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“…In this regard, we have previously highlighted that the amino acid composition of OEE is characterized by a predominance of hydrophobic amino acids [18] which have been associated with the antioxidant activity of bioactive peptides [26,69,71,72]. Besides, the hydrolysis of proteins results in an elevation of the concentration of sulfur amino acids, namely methionine and cysteine, both of which possess signi cant antioxidant potential [73]. Furthermore, the peptides in OEE have a direct relationship with iso avones.…”

Section: Principio Del Formulariomentioning

confidence: 99%

A new biostimulant derived from soybean by-products enhances plant tolerance to abiotic stress triggered by ozone

Orts,

Navarro-Torre,

Macías-Benítez

et al. 2024

Preprint

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Background Tropospheric ozone is an air pollutant that causes negative effects on vegetation, leading to significant losses in crop productivity. It is generated by chemical reactions in the presence of sunlight between primary pollutants resulting from human activity, such as nitrogen oxides and volatile organic compounds. Due to the constantly increasing emission of ozone precursors, together with the influence of a warming climate on ozone levels, crop losses may be aggravated in the future. Therefore, the search for solutions to mitigate these losses becomes a priority. Ozone-induced abiotic stress is mainly due to reactive oxygen species (ROS) generated by the spontaneous decomposition of ozone once it reaches the apoplast. In this regard, compounds with antioxidant activity offer a viable option to alleviate ozone-induced damage. Using enzymatic technology, we have developed a process that enables the production of an extract with biostimulant properties from okara, an industrial soybean byproduct. The biostimulant, named as OEE (Okara Enzymatic Extract), is water-soluble and is enriched in bioactive compounds present in okara, such as isoflavones. Additionally, it contains a significant fraction of protein hydrolysates contributing to its functional effect. Given its antioxidant capacity, we aimed to investigate whether OEE could alleviate ozone-induced damage in plants. For that, pepper plants (Capsicum annuum) exposed to ozone were treated with a foliar application of OEE. Results OEE provided protection against ozone-induced damage, as evidenced by the net photosynthetic rate (AN), electron transport rate (ETR), effective quantum yield of PSII (PhiPS2), and delayed fluorescence (DF). The protective effect was mainly attributed to its antioxidant activity, as indicated the lipid peroxidation rate assayed by malondialdehyde (MDA) content. OEE also induced a mild oxidative response, as demonstrated by the increased activities of antioxidant enzymes in leaves (catalase, superoxide dismutase, and guaiacol peroxidase) and the oxidative stress index (OSI), which further protected against ozone-induced stress. Conclusions The present results support that OEE protects plants from ozone exposure. Taking into consideration that the promotion of plant resistance against abiotic damage is an important goal of biostimulants, we assume that its use as a new biostimulant could be considered.

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“…In this regard, we have previously highlighted that the amino acid composition of OEE is characterized by a predominance of hydrophobic amino acids [18] which have been associated with the antioxidant activity of bioactive peptides [31,74,76,77]. Besides, the hydrolysis of proteins results in an elevation of the concentration of sulfur amino acids, namely methionine and cysteine, both of which possess significant antioxidant potential [78]. Furthermore, the peptides in OEE have a direct relationship with isoflavones.…”

Section: Principio Del Formulariomentioning

confidence: 99%

A new biostimulant derived from soybean by-products enhances plant tolerance to abiotic stress triggered by ozone

Orts,

Navarro-Torre,

Macías-Benítez

et al. 2024

BMC Plant Biol

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Background Tropospheric ozone is an air pollutant that causes negative effects on vegetation, leading to significant losses in crop productivity. It is generated by chemical reactions in the presence of sunlight between primary pollutants resulting from human activity, such as nitrogen oxides and volatile organic compounds. Due to the constantly increasing emission of ozone precursors, together with the influence of a warming climate on ozone levels, crop losses may be aggravated in the future. Therefore, the search for solutions to mitigate these losses becomes a priority. Ozone-induced abiotic stress is mainly due to reactive oxygen species generated by the spontaneous decomposition of ozone once it reaches the apoplast. In this regard, compounds with antioxidant activity offer a viable option to alleviate ozone-induced damage. Using enzymatic technology, we have developed a process that enables the production of an extract with biostimulant properties from okara, an industrial soybean byproduct. The biostimulant, named as OEE (Okara Enzymatic Extract), is water-soluble and is enriched in bioactive compounds present in okara, such as isoflavones. Additionally, it contains a significant fraction of protein hydrolysates contributing to its functional effect. Given its antioxidant capacity, we aimed to investigate whether OEE could alleviate ozone-induced damage in plants. For that, pepper plants (Capsicum annuum) exposed to ozone were treated with a foliar application of OEE. Results OEE mitigated ozone-induced damage, as evidenced by the net photosynthetic rate, electron transport rate, effective quantum yield of PSII, and delayed fluorescence. This protection was confirmed by the level of expression of genes associated with photosystem II. The beneficial effect was primarily due to its antioxidant activity, as evidenced by the lipid peroxidation rate measured through malondialdehyde content. Additionally, OEE triggered a mild oxidative response, indicated by increased activities of antioxidant enzymes in leaves (catalase, superoxide dismutase, and guaiacol peroxidase) and the oxidative stress index, providing further protection against ozone-induced stress. Conclusions The present results support that OEE protects plants from ozone exposure. Taking into consideration that the promotion of plant resistance against abiotic damage is an important goal of biostimulants, we assume that its use as a new biostimulant could be considered.

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Enzymatic hydrolysis of okara protein concentrate by mixture of endo and exopeptidase

Cited by 15 publications

References 29 publications

Soil carbon dioxide efflux from amended saline and thermally desorbed oil‐contaminated subsoil

Soil carbon dioxide efflux from amended saline and thermally desorbed oil‐contaminated subsoil

A new biostimulant derived from soybean by-products enhances plant tolerance to abiotic stress triggered by ozone

A new biostimulant derived from soybean by-products enhances plant tolerance to abiotic stress triggered by ozone

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