Enzymatic hydrolysis of pretreated Alfa fibers ( Stipa tenacissima ) using β- d -glucosidase and xylanase of  Talaromyces thermophilus  from solid-state fermentation

Mallek-Fakhfakh, Hanen; Fakhfakh, Jawhar; Walha, Khaled; Hassairi, Hajer; Gargouri, Ali; Belghith, Hafedh

doi:10.1016/j.ijbiomac.2017.05.078

Cited by 21 publications

(7 citation statements)

References 48 publications

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“…In summary, the optimum fermentation time for enzyme production by P. oxalicum M1816 was determined to be 96 h. Water is essential for microbial growth. The moisture content is one of the most ical factors that influences enzymatic production under SSF [63]. The effect of the in moisture content on the activities of different enzymes is shown in Figure 4B.…”

Section: Optimization Of the Ssf Conditions By P Oxalicum M1816 For Enzyme Productionmentioning

confidence: 99%

“…The CMCase and xylanase activities increased firstly and decreased with the change of initial pH, and reached the maximum activities at pH Water is essential for microbial growth. The moisture content is one of the most critical factors that influences enzymatic production under SSF [63]. The effect of the initial moisture content on the activities of different enzymes is shown in Figure 4B.…”

Section: Optimization Of the Ssf Conditions By P Oxalicum M1816 For Enzyme Productionmentioning

confidence: 99%

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Enzyme Production Potential of Penicillium oxalicum M1816 and Its Application in Ferulic Acid Production

Zhang

Liu

Hai-long

et al. 2021

Foods

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The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as Penicillium oxalicum M1816. The genome was sequenced and assembled into 30.5 Mb containing 8301 predicted protein-coding genes. In total, 553 genes were associated with carbohydrate metabolism. Genomic CAZymes analysis indicated that P. oxalicum M1816, comprising 39 cellulolytic enzymes and 111 hemicellulases (including 5 feruloyl esterase genes), may play a vital role in wheat bran degradation and FA production. The crude enzyme of strain M1816 could release 1.85 ± 0.08 mg·g−1 FA from de-starched wheat bran (DSWB) at 12 h, which was significantly higher than other commercial enzymes. Meanwhile, when the strain M1816 was cultured in medium supplemented with DSWB, up to 92.89% of the total alkali-extractable FA was released. The process parameters of solid-state fermentation were optimized to enhance enzyme production. The optimized wheat bran Qu of P. oxalicum M1816 was applied to huangjiu fermentation, and the FA content was increased 12.4-fold compared to the control group. These results suggest that P. oxalicum M1816 is a good candidate for the development of fermented foods bio-fortified with FA.

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Section: Optimization Of the Ssf Conditions By P Oxalicum M1816 For Enzyme Productionmentioning

confidence: 99%

Section: Optimization Of the Ssf Conditions By P Oxalicum M1816 For Enzyme Productionmentioning

confidence: 99%

Enzyme Production Potential of Penicillium oxalicum M1816 and Its Application in Ferulic Acid Production

Zhang

Liu

Hai-long

et al. 2021

Foods

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“…Under appropriate fermentation conditions including temperature, oxygen levels, and pH, microbial species can utilize carbohydrates, proteins, and fats found in raw ingredients. They secrete enzymes such as fiber hydrolase, glucosidase, and xylanase, which hydrolyze the macromolecular insoluble dietary fiber into smaller soluble dietary fiber . Furthermore, microbial-produced acidic metabolites can also convert some insoluble dietary fiber into soluble dietary fiber by breaking down glycosidic bonds .…”

Section: Strategies For Enhancing the Biological Activity Of Dietary ...mentioning

confidence: 99%

Dietary Fiber and Its Potential Role in Obesity: A Focus on Modulating the Gut Microbiota

Tian,

Chu,

et al. 2023

J. Agric. Food Chem.

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Dietary fiber is a carbohydrate polymer with ten or more monomeric units that are resistant to digestion by human digestive enzymes, and it has gained widespread attention due to its significant role in health improvement through regulating gut microbiota. In this review, we summarized the interaction between dietary fiber, gut microbiota, and obesity, and the beneficial effects of dietary fiber on obesity through the modulation of microbiota, such as modifying selective microbial composition, producing starch-degrading enzymes, improving gut barrier function, reducing the inflammatory response, reducing trimethylamine N-oxide, and promoting the production of gut microbial metabolites (e.g., short chain fatty acids, bile acids, ferulic acid, and succinate). In addition, factors affecting the gut microbiota composition and metabolites by dietary fiber (length of the chain, monosaccharide composition, glycosidic bonds) were also concluded. Moreover, strategies for enhancing the biological activity of dietary fiber (fermentation technology, ultrasonic modification, nanotechnology, and microfluidization) were subsequently discussed. This review may provide clues for deeply exploring the structure–activity relationship between dietary fiber and antiobesity properties by targeting specific gut microbiota.

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“…[10][11][12]. Ксиланазы также хорошо подходят для клонирования и гомологичной и гетерологичной экспрессии, а штаммамихозяевами являются Escherichia coli [13,14], Bacillus subtilis [15], Pichia pastoris [16,17], Pichia stipites [18]. Высокий потенциал ксиланаз для переработки рас-тительной биомассы стимулирует поиск новых ферментов и разработку новых технологий.…”

Section: абстрактunclassified

Study of the Biochemical Characteristics of Glycosylated Recombinant Xylanase

Kiribayeva¹,

Silayev²,

Abdullayeva³

et al. 2022

Eurasian Journal of Applied Biotechnology

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The main renewable source of energy and raw materials on Earth is plant biomass, most of which consists of plant cell wall polymers: cellulose, hemicellulose, and lignin. Hemicelluloses are divided into three main types: xylans, mannans and arabinogalactans. Xylan is the second most abundant carbohydrate in nature after cellulose. The specific enzymes that hydrolyze xylan into xylooligosaccharides and D-xylose are xylanases. Prospective xylanases are enzymes derived from bacteria and mycelial fungi. Bacterial xylanases are characterized by high unitary activity, and additional manose chains can significantly increase the stability of the enzyme protein globule. Obtaining a glycosylated variant of bacillary xylanase, which are known for their high specific activity, seems promising. The Bacillus sonorensis T6 xylanase gene was cloned and expressed in the yeast Pichia pastoris. The recombinant xylanase was isolated and purified. The biochemical characteristics of the glycosylated recombinant xylanase were studied. It was found that the recombinant xylanase had maximum activity at 47-50°C and pH 6.0. The Km, Vmax and Kcat values are 3.037 ± 0.362 (mg/ml), 667.8 ± 31 (units/mg) and 100.3 ± 4.6 (s-1), respectively. The unitary activity of the recombinant enzyme is 873.8 units/mg. The glycosylated recombinant xylanase was found to have high temperature stability and retained 47% activity after a 2-hour incubation at 55°C. In addition to temperature stability, recombinant xylanase showed high pH stability - 10 h incubation in buffers with pH 3-11 did not decrease the activity. The effect of metal ions, detergents, and organic solvents on the activity of recombinant glycosylated xylanase was studied. The high biochemical parameters of recombinant glycosylated xylanase from Bacillus sonorensis T6 suggest that this enzyme is promising for use in the biotechnology and food industry.

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Enzymatic hydrolysis of pretreated Alfa fibers ( Stipa tenacissima ) using β- d -glucosidase and xylanase of Talaromyces thermophilus from solid-state fermentation

Cited by 21 publications

References 48 publications

Enzyme Production Potential of Penicillium oxalicum M1816 and Its Application in Ferulic Acid Production

Enzyme Production Potential of Penicillium oxalicum M1816 and Its Application in Ferulic Acid Production

Dietary Fiber and Its Potential Role in Obesity: A Focus on Modulating the Gut Microbiota

Study of the Biochemical Characteristics of Glycosylated Recombinant Xylanase

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