Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder

Abstract

A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA).

Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6

“…Thus, prolong the hydrolysis time will increase the glucose production. Similar trend was obtained by Tan and Lee 16 and Zheng et al 13 A comparison of results based on two different hydrolysis time reveals glucan and time are interrelated in increasing the glucose production. …”

“…The acid insoluble material was determined using gravimetric analysis while UV-Vis spectroscopy was used to measure acid soluble lignin in FPOPF. 13 …”

Microwave-assisted Dilute Acid Pretreatment and Enzymatic Hydrolysis of Sago Palm Bark

“…In general, the relationship between the crystallinity index, the change in crystallite size of pretreated biomass, and its corresponding enzymatic hydrolysis rate is not well understood. Biomass with a high crystallinity index may not necessarily negatively affect the enzymatic hydrolysis rate (Kim and Holtzapple 2006;Zheng et al 2014).…”

Liquid hot water pretreatment of multi feedstocks and enzymatic hydrolysis of solids obtained thereof

“…While CC exhibits a highly fibrillar, ordered and rigid structure with a relatively smooth surface(Zheng et al, 2014), CH presents cellulose fibers with a structure considered too short and/or weak(Reddy and Yang, 2007). These LCMs are considered a potential feedstock for cellulosic ethanol production due to their low lignin and high carbohydrate contents.These were confirmed by the obtained results, with CC presenting 35.8% cellulose, 30.7% hemicellulose and a lignin content of 18.5%, while CH presented 32.5% cellulose, 30.4% hemicellulose and 15.5% lignin.…”