Enzymatic hydrolysis of the<i>Eisenia andrei</i>earthworm: Characterization and evaluation of its properties

Rodrigues, Mariano; Carlesso, Wagner Manica; Kuhn, Daniel; Altmayer, Taciélen; Martini, Maira Cristina; Tamiosso, Camila Durlo Departamento de Medicina Veterinária Preventiva; Mallmann, Carlos Augusto; Souza, Cláucia Fernanda Volken de; Ethur, Eduardo Miranda; Hoehne, Lucélia

doi:10.1080/10242422.2017.1278754

Cited by 11 publications

(15 citation statements)

References 56 publications

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“…La pasta presenta un alto contenido de proteína en comparación con sus demás macro compuesto, siendo esta principalmente albuminas, globulinas y glutelinas con un 65,25%, 20,12% y 11,87% respectivamente. Este valor de proteína se encuentra en el rango normal de acuerdo con lo reportado en la literatura, en la que se menciona que la lombriz de tierra presenta valores de proteína superiores al 60% (Muller et al, 2017;Rodrigues et al, 2017). Adicionalmente se resalta el alto contenido de minerales con un 5,82% en la composición nutricional.…”

Section: Resultados Y Discusiónunclassified

“…En este contexto la lombricultura surge como una opción de gran viabilidad, ya que es un proceso biológico en el que los subproductos vegetales y residuos agrícolas, se transforman en productos de alto valor en la agricultura como es el caso del humus líquido y sólido. Esta actividad ha presentado un crecimiento acelerado en los últimos años debido a su facilidad de cuidado de la lombriz, rápida reproducción y crecimiento (Rodrigues et al, 2017). Diferentes especies de lombriz de tierra se han empleado desde mucho tiempo atrás principalmente para la obtención de abono orgánico, pero pueden también considerarse una excelente fuente de proteína de compuestos biológicos y farmacológicos activos, que han sido empleados en el tratamiento de diversas enfermedades, principalmente por las culturas China e hindú (Sun, 2015).…”

Section: Introductionunclassified

“…La hidrolisis enzimática es la metodología más común y ampliamente utilizada para obtener péptidos bioactivos ya que usa condiciones de operación moderadas, con cierto grado de especificidad por sustrato (Suarez et al, 2021). Por otro lado, se han investigado diferentes especies de lombriz de tierra como Eisenia Andrei (Rodrigues et al, 2017), Lumbricus rubellus (Azmi et al, 2014) entre otras, para la obtención de péptidos mediante procesos de hidrolisis enzimática con diferentes propiedades biológicas como capacidad antioxidante, actividad antimicrobiana, entre otras. Sin embargo, se presentan pocos informes en la literatura sobre hidrolizados enzimáticos de lombriz roja californiana (Eisenia fétida) o de su líquido celomático y sus posibles propiedades biológicas (Qin et al, 2010).…”

Section: Introductionunclassified

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Efecto del fraccionamiento con membranas sobre la actividad biológica de hidrolizados enzimáticos de proteína de lombriz roja californiana (Eisenia foetida)

Gaviria

Montoya

2023

Inf. tecnol.

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El principal objetivo del presente trabajo es evaluar el efecto de la separación con membranas sobre la actividad biológica de hidrolizados enzimáticos de proteínas de lombriz roja californiana (Eisenia foetida). Para la hidrólisis enzimática se usa un reactor de vidrio 0.5 L. El fraccionamiento se realiza en un sistema de ultrafiltración tangencial con membranas de 1kDa y 3kDa. Al hidrolizado y sus fracciones se les determinan sus capacidades antioxidantes y la capacidad de quelación de hierro. Los resultados muestran que el componente mayoritario de la pasta es proteína, siendo las albuminas el 65%. Por otra parte, la mayor reducción de flux de permeado se da en la membrana de 1kDa (19 L/m2h), presentando bloqueo completo de poros como mecanismo de ensuciamiento. En conclusión, la fracción <3kDa presenta la mayor capacidad antioxidante y quelante de hierro, mientras que la filtración en membranas <1kDa presenta la mayor reducción de flux de permeado.

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Section: Resultados Y Discusiónunclassified

Section: Introductionunclassified

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Efecto del fraccionamiento con membranas sobre la actividad biológica de hidrolizados enzimáticos de proteína de lombriz roja californiana (Eisenia foetida)

Gaviria

Montoya

2023

Inf. tecnol.

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“…The central composite design (CCD) was conducted in the optimum condition of the significant factors for the protein hydrolysis under response surface methodology (RSM; Rodrigues et al, 2017 ). In this experimental design, optimization is handled by varying two factors: the E/S concentration ratio [A: 1%–3% (v/v)] and the time of this hydrolysis (B: 1–3 h).…”

Section: Methodsmentioning

confidence: 99%

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

et al. 2022

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Earthworms have long been used as traditional medicine. The purposes of this research were to create bioactive peptides from the unique Amynthas arenulus earthworm (PAAEs) and test their potentials on liver cancer bioprophylactic activity, antioxidant, oxidative stress protection, and immune cell activation. This earthworm had a high protein content ratio, at 55.39%. Besides, PM 35 is one out of 58 bacteria isolated from the earthworm carcasses that exhibited the highest protease and yield protein production which was chosen as the protease-producing bacteria to hydrolyze the protein. The genera were identified by 16S rRNA and 16S–23S rRNA comparison and confirmed as Bacillus velezensis PM 35. The response surface methodology was applied to optimize these hydrolysis parameters, i.e., the enzyme/substrate (E/S) concentration ratio [1%–3% (v/v)] and time (1–3 h) of the hydrolyzing earthworm’s proteins. The optimal hydrolyzing conditions were 3% (v/v) of E/S concentration ratio and 3 h of hydrolysis time, which found protein-hydrolysate yield (24.62%) and degree of hydrolysis (85.45%) as the highest. After being challenged in the gastrointestinal tract-resistant model, these PAAEs (MW <3 and 3–5 kDa) induced liver cancer cell (HepG2) death via apoptotic action modes (cell morphological change and DNA fragmentation). The PAAEs (MW <3 kDa) exhibited significant antioxidant activity via DPPH, ABTS, and FRAP with IC50 values of 0.94, 0.44, and 6.34 mg/ml, respectively. The PAAEs (MW < 3 kDa) were non-cytotoxic and protected the mouse fibroblast cells (L929) against oxidative stress. These PAAEs (MW < 3 kDa, 0.2 mg/ml) stimulated the B lymphocytes (122.3%), and T lymphocytes (126.7%) proliferation. This research suggests that PAAEs can be used in a variety of applications, especially in the food and pharmaceutical industries.

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“…Earthworms are the natural source of protein therapeutics. These species contain up to 65% of the total protein content (Rodrigues et al, 2017). DLBS1033N protein fraction of Lumbricus rubellus was developed by hydrolysis technique of DLBS1033 (Trisina et al, 2011) which is supposed to produce low molecular weight protein.…”

Section: Introductionmentioning

confidence: 99%

Enhancement Peripheral Regeneration as a Target of Potential Diabetic Neuropathy Treatment from Lumbricus rubellus Fraction DLBS1033N: the role of cell viability and migration

Hartanti

Nugroho²,

Tjandrawinata³

2022

Indonesian J Pharm

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Diabetic Peripheral Neuropathy (DPN) significantly affects the quality of life with no definitive therapy currently. Given the pathologic basis for DPN treatment, it's critical to promote neuron regeneration while also restricting nerve degeneration. Schwann cells that play pivotal roles against peripheral regeneration manifest cell proliferation and survival inhibition in diabetic patients consecutively decreased peripheral regeneration capacity. DLBS1033N, a protein hydrolysate obtained from Lumbricus rubellus, has been confirmed to promote Schwann cell line RSC96 growth and survival by induction Nerve Growth Factor (NGF) expression via phosphatidylinositol-3‑kinase (PI3K) pathway. This pathway has an important contribution against Schwann cell proliferation and migration. Herein, the contribution of DLBS1033N to peripheral regeneration on high-glucose (50mM)-induced rat Schwann cell line RSC96 injury, a well-known DPN in vitro cell model. RSC96 were treated with high glucose (50mM) with or without DLBS1033N 25, 50, and 100μg/mL for 24, 48, and 72 h. MTS assay kit were used to evaluate cell viability. DLBS1033N significantly improved cell proliferation in 48 h incubation time with a dose-dependent manner (p < 0.05). Furthermore, DLBS1033N 100μg/ml significantly promoted cell migration by 16% in 48 H incubation (p < 0.05) determined by scratch assay, as the beneficial action to accomplish peripheral regeneration. In conclusion, DLBS1033N enhanced peripheral regeneration which could be used as an effective and promising DPN treatment.

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Enzymatic hydrolysis of theEisenia andreiearthworm: Characterization and evaluation of its properties

Cited by 11 publications

References 56 publications

Efecto del fraccionamiento con membranas sobre la actividad biológica de hidrolizados enzimáticos de proteína de lombriz roja californiana (Eisenia foetida)

Efecto del fraccionamiento con membranas sobre la actividad biológica de hidrolizados enzimáticos de proteína de lombriz roja californiana (Eisenia foetida)

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

Enhancement Peripheral Regeneration as a Target of Potential Diabetic Neuropathy Treatment from Lumbricus rubellus Fraction DLBS1033N: the role of cell viability and migration

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