Enzymatic hydrolysis of wheat straw: Kinetic analysis

Aguado, J.; Romero, M.D.; Moncó, G.; Rodríguez, L.

doi:10.1002/abio.370130105

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…Over time, various mechanisms have been proposed for cellulose hydrolysis by cellulose degrading enzymes, including MichaelisÁMenten-like kinetics (Beltrame et al 1984;Gonzalez et al 1989), models that consider enzyme adsorption onto cellulose (Scheiding et al 1984;Philippidis et al 1992b;, and semi-empirical models (Aguado et al 1993). These models, however, sometimes do not consider effects occurring at increased conversions and/or prolonged reaction times (e.g.…”

Section: Outline Reaction Mechanismmentioning

confidence: 99%

A generic model for glucose production from various cellulose sources by a commercial cellulase complex

Drissen

Maas

Maarel³

et al. 2007

Biocatalysis and Biotransformation

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The kinetics of cellulose hydrolysis by commercially available Cellubrix were described mathematically, with Avicel and wheat straw as substrates. It was demonstrated that hydrolysis could be described by three reactions: direct glucose formation and indirect glucose formation via cellobiose. Hydrolysis did not involve any soluble oligomers apart from low amounts of cellobiose. Phenomena included in the mathematical model were substrate limitation, adsorption of enzyme onto substrate, glucose inhibition, temperature dependency of reaction rates, and thermal enzyme inactivation. In addition, substrate heterogeneity was described by a recalcitrance constant. Model parameters refer to both enzyme characteristics and substrate-specific characteristics.Quantitative model development was carried out on the basis of Avicel hydrolysis. In order to describe wheat straw hydrolysis, wheat straw specific parameter values were measured. Updating the pertinent parameters for wheat straw yielded a satisfactory description of wheat straw hydrolysis, thus underlining the generic potential of the model.

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Section: Outline Reaction Mechanismmentioning

confidence: 99%

A generic model for glucose production from various cellulose sources by a commercial cellulase complex

Drissen

Maas

Maarel³

et al. 2007

Biocatalysis and Biotransformation

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Kinetic Measurements of Cellulase Activity on Insoluble Substrates Using Disodium 2,2' Bicinchoninate

Johnston

Shoemaker

Smith

et al. 1998

J Food Biochemistry

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Determinations of cellulase activity using insoluble substrates have typically required long incubation times and significant amounts of enzyme. Utilizing disodium 2,2′‐bicinchoninate, an assay has been developed to measure the initial production of reducing groups released during cellulase hydrolysis of insoluble, regenerated cellulose. The procedure permits the determination of total, soluble and insoluble reducing groups from a single reaction mixture under initial velocity conditions (< 3% hydrolysis). We report the applicability of this method to determinations of enzyme activity and for average molecular weight calculations of substrates. The K m values for Trichoderma reesei EG I, EG II, CBH I and CBH II are reported using phosphoric acid swollen cellulose (average DP 166) as a substrate. Procedures for preparation of uniform cellulose suspensions are described.

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Enzymatic hydrolysis of wheat straw: Kinetic analysis

Cited by 2 publications

References 20 publications

A generic model for glucose production from various cellulose sources by a commercial cellulase complex

A generic model for glucose production from various cellulose sources by a commercial cellulase complex

Kinetic Measurements of Cellulase Activity on Insoluble Substrates Using Disodium 2,2' Bicinchoninate

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