Enzymatic hydrolysis optimization of <i>Paphia undulata</i> and lymphocyte proliferation activity of the isolated peptide fractions

He, Xuesong; Cao, Wen; Pan, Guang Kun; Yang, Liting; Zhang, Chao Hua

doi:10.1002/jsfa.6859

Cited by 50 publications

(17 citation statements)

References 33 publications

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“…This species is highly popular in China because of its delicacy and low price. The bioactivities of P. undulate peptides produced by enzymatic hydrolyzation were recently revealed (He et al, 2015). Our preliminary studies focused on the enzymolysis conditions for P. undulate peptide preparation (Xin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identification of odor volatile compounds and deodorization of Paphia undulata enzymatic hydrolysate

Chen

et al. 2016

J. Ocean Univ. China

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Unfavorable fishy odour is an inevitable problem in aquatic products. In the present study, headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS) analysis of volatiles from untreated samples and three deodorized samples (under the optimal conditions) of Paphia undulata enzymatic hydrolysate revealed that the compounds contributing to the distinctive odor were 1-octen-3-ol, n-hexanal, n-heptanal, 2,4-heptadienal, and 2,4-decadienal, whereas n-pentanal, n-octanal, n-octanol, benzaldehyde, 2-ethylfuran and 2-pentylfuran were the main contributors to the aromatic flavor. The deodorizing effects of activated carbon (AC) adsorption, yeast extract (YE) masking and tea polyphenol (TP) treatment on a P. undulata enzymatic hydrolysate were investigated using orthogonal experiments with sensory evaluation as the index. The following optimized deodorization conditions were obtained: AC adsorption (35 mg mL −1 , 80℃, 40 min), YE masking (7 mg mL −1 , 45℃, 30 min) and TP treatment (0.4 mg mL −1 , 40℃, 50 min). AC adsorption effectively removed off-flavor volatile aldehydes and ketones. YE masking modified the odor profile by increasing the relative contents of aromatic compounds and decreasing the relative contents of aldehydes and ketones. The TP treatment was not effective in reducing the odor score, but it significantly reduced the relative content of aldehydes while increasing that of alkanes. It is also notable that TP effectively suppressed trimethylamine (TMA) formation in a P. undulate hydrolysate solution for a period of 72 h.

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Section: Introductionmentioning

confidence: 99%

Identification of odor volatile compounds and deodorization of Paphia undulata enzymatic hydrolysate

Chen

et al. 2016

J. Ocean Univ. China

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“…However, with the further increase in the enzyme dosage, the lymphocyte proliferation rate of CII peptides significantly decreased (p < 0.05), which may be due to the formation of more small peptides and free amino acids. Compared with the immunomodulatory peptide isolated by other methods (He, Cao, Pan, Yang, & Zhang, 2015;Hou et al, 2012b), DH and activity of CII peptide isolated by a CIEH-MS system have been significantly improved.…”

Section: Dh and Activity Of CII Peptidementioning

confidence: 99%

Continuous preparation and characterization of immunomodulatory peptides from type II collagen by a novel immobilized enzyme membrane reactor with improved performance

Cao

Zhang

et al. 2019

J Food Biochem

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In this study, a novel method of continuous coupling of immobilized enzymatic hydrolysis reactor and membrane separation (CIEH‐MS) was used to isolate the immunomodulatory peptides from type II collagen (CII) in chick sternal cartilage. The immobilized neutral protease was successfully prepared with an activity of 400.5 U/g. The CIEH‐MS system loaded with immobilized neutral protease had high operational stability with enzyme decay constant of 0.0077 and half‐life of 89.61 hr. Using a CIEH‐MS system, the immunomodulatory peptides were obtained with lymphocyte proliferation of 66.23%, peptide yield of 23.43%, degree of hydrolysis (DH) of 22.41%, and permeate flux of 6.17 L/m2h. The peptide fractions were further purified and the P3‐2‐4 fraction (RGQLGPM) with 760.4 Da molecular weights exhibited the highest lymphocyte proliferation activity (85.54%) and binding ability to human leukocyte antigen‐DRB1 (HLA‐DRB1) molecules (133.2 ng/ml). The results demonstrated that CIEH‐MS system is an effective way to obtain immunomodulatory peptides from CII. Practical applications Chick sternal cartilage is one of the by‐products of meat processing, and it is often discarded as waste or used for low‐value purposes. CII is the most abundant collagen in chick sternal cartilage, and recently studies have demonstrated that CII peptides possess the ability to induce immunologic tolerance for the treatment of chronic disease. In order to find potential applications for this by‐product, immunomodulatory peptides from CII hydrolysates in chick sternal cartilage were isolated using a novel CIEH‐MS system. The result showed that CII peptides exhibited a high immunomodulatory activity, and had a potential application in functional foods and medical fields.

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“…An increase in lymphocyte proliferation was observed after stimulation with other protein hydrolysates which were derived from soy, wheat, whey, casein, and mollusk [ 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. Not all protein hydrolysates were found to stimulate proliferation.…”

Section: Immune Effects Of Hydrolysatesmentioning

confidence: 99%

Immunomodulatory Protein Hydrolysates and Their Application

2018

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Immunomodulatory protein hydrolysate consumption may delay or prevent western immune-related diseases. In order to purposively develop protein hydrolysates with an optimal and reproducible immunomodulatory effect, knowledge is needed on which components in protein hydrolysates are responsible for the immune effects. Important advances have been made on this aspect. Also, knowledge on mechanisms underlying the immune modulating effects is indispensable. In this review, we discuss the most promising application possibilities for immunomodulatory protein hydrolysates. In order to do so, an overview is provided on reported in vivo immune effects of protein hydrolysates in both local intestinal and systemic organs, and the current insights in the underlying mechanisms of these effects. Furthermore, we discuss current knowledge and physicochemical approaches to identify the immune active protein sequence(s). We conclude that multiple hydrolysate compositions show specific immune effects. This knowledge can improve the efficacy of existing hydrolysate-containing products such as sports nutrition, clinical nutrition, and infant formula. We also provide arguments for why immunomodulatory protein hydrolysates could be applied to manage the immune response in the increasing number of individuals with a higher risk of immune dysfunction due to, for example, increasing age or stress.

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Enzymatic hydrolysis optimization of Paphia undulata and lymphocyte proliferation activity of the isolated peptide fractions

Cited by 50 publications

References 33 publications

Identification of odor volatile compounds and deodorization of Paphia undulata enzymatic hydrolysate

Identification of odor volatile compounds and deodorization of Paphia undulata enzymatic hydrolysate

Continuous preparation and characterization of immunomodulatory peptides from type II collagen by a novel immobilized enzyme membrane reactor with improved performance

Immunomodulatory Protein Hydrolysates and Their Application

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