Enzymatic Improvement in the Polyphenol Extractability and Antioxidant Activity of Green Tea Extracts

Hong, Yang; Jung, Eun Young; Park, Yooheon; Shin, Kihyun; Kim, Tae Young; Yu, Kwang-Won; Chang, Un Jae; Suh, Hyung Joo

doi:10.1271/bbb.120373

Cited by 57 publications

(46 citation statements)

References 48 publications

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“…This result affirmed efficiency of EAE to collect bioactive compounds. Our results are similar to some previously published papers, confirming the effectiveness of the EAE in improving the efficient retrieval of bioactive compounds [5,6].…”

Section: Comparison Of Uae With Cesupporting

confidence: 91%

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USING CELLULASE TO IMPROVE THE EXTRACTION EFFICIENCY OF THE ANTIOXIDANT COMPOUNDS FROM WHITE RADISH (Raphanus sativus L.) POWDER

Ngoc

2018

JST

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White radish (Raphanus sativus L.) is an edible root vegetable of the Brassicaceae family. It is cultivated and consumed all over the world. Nowadays, radishes are used and eaten by many people in their daily meals. Some studies showed that it has a high content of antioxidant compounds. In this study, we used enzyme -assisted extraction (EAE) to produce an antioxidant -rich extract. The antioxidant activity of the extract was determined by the ABTS radical cation scavenging capacity. The optimal extraction conditions of the antioxidant compounds from white radish were: enzyme concentration of 1.75 % (v/w), extraction time of 81 minutes, pH 4.56 and extraction temperature of 48 o C. As a result, the antioxidant activity of the extract was 0.1594 ± 0.033 m Moltrolox equivalent antioxidant capacity (TEAC)/g and 161 % higher than that of the control sample(CE).Furthermore, SEM (scanning electron microscopy) analysis result showed that there was a difference in the surface structure of white radish powder after being extracted by CE and EAE.

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Section: Comparison Of Uae With Cesupporting

confidence: 91%

“…This method has been reported for extracting the phenolics from green tea and citrus peel [5,6]. The mechanism of EAE is that enzymes have the ability to degrade or disrupt cell walls and membranes, thus it enables better release and more efficient extraction of bioactivities [7].…”

Section: Introductionmentioning

confidence: 99%

USING CELLULASE TO IMPROVE THE EXTRACTION EFFICIENCY OF THE ANTIOXIDANT COMPOUNDS FROM WHITE RADISH (Raphanus sativus L.) POWDER

Ngoc

2018

JST

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“…These phenomena may suggest that Viscozyme ® L also hydrolysed carbohydrates that formed a complex with the more hydrophobic phenolic compounds (Hong et al, 2013). This hydrolysis leads to free phenolic compounds again, with decreased solubility in water and increased solubility in 50% ethanol.…”

Section: Influence Of Pre-treatment On Protein Color Formationmentioning

confidence: 99%

Improving yield and composition of protein concentrates from green tea residue in an agri-food supply chain: Effect of pre-treatment

Zhang

Krimpen

Sanders

et al. 2016

Food and Bioproducts Processing

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“…Swollen mitochondria with disrupted cristae (Fig. epithelial height and colloid area of thyroid follicles were measured in pixel and pixel [2] respectively under high power (×400). 10b).…”

Section: Electron Microscopic Resultsmentioning

confidence: 99%

“…More recently, GT extracts enriched with certain compounds are also available as dietary supplements in the form of tablets and capsules [7]. EGCG represents the most abundant of tea catechins (50-80% of total catechins) that have excellent antioxidant activities, as evidenced by the fact that their free-radical scavenging ability is more powerful than that of vitamin C, vitamin E, or β-carotene [2]. The four major catechins in GT are epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate, epigallocatechin, and epicatechin [1,8].…”

Section: Morphometric Studymentioning

confidence: 99%

Light and electron microscopic study of thyroid follicular cells in adult albino rats following green tea administration

Mahmoud¹,

Solaiman²

2014

The Egyptian Journal of Histology

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BackgroundGreen tea (GT) is one of the most popular beverages with high daily consumption worldwide. It has a rich content of polyphenols, among which catechins predominate. Its extracts are widely used as ingredients in dietary supplements, beverages, and functional foods, which may lead to a higher consumption of GT polyphenols by the general population. Recently, a relationship between GT administration and disruption of thyroid homeostasis was suggested. AimThe goal of the study was to clarify the impact of GT administration on the histological structure of thyroid follicular cells of adult albino rats. Materials and methodsThirty adult male albino rats (140-160 g) were categorized into three equal groups: group I served as the control group; groups II and III received 1.25 and 5.0 g% GT extract orally at a dose of 10 ml/kg body weight, respectively, every day for 30 days. At the end of the experiment, blood samples were collected from all animals for estimation of serum triiodothyronine (T 3 ), thyroxine (T 4 ), and thyroid stimulating hormone. Rats were then euthanized and the thyroid glands were harvested for histological examination of thyroid follicular cells by light and transmission electron microscopy. The follicular epithelial height and colloid area of thyroid follicles were morphometrically evaluated and statistically analyzed. ResultsGT in small concentrations (in group II) did not induce any significant change in hormonal, histological, and histomorphometric assessments as compared with the control group. However, some follicular cells exhibited mildly dilated rough endoplasmic reticulum and multiple lysosomes. In contrast, GT administration in group III induced hypothyroidism as evidenced by significant reduction in serum T 3 and T 4 levels along with substantial increase in thyroid stimulating hormone level compared with controls. Histologically, many follicles displayed signs of hyperactivity as evidenced by focal hypertrophy and hyperplasia of the follicular cells along with colloid depletion. Ultrastructurally, dilated rough endoplasmic reticulum and disrupted mitochondria were encountered. Some severely degenerated follicular cells were revealed as well. Histomorphometrically, there was a significant increase in the mean follicular epithelial height, whereas the colloid area was significantly lowered as compared with the respective controls. ConclusionThe study clearly indicates that GT in large concentrations has the potential to disrupt thyroid follicular cell structure and function.

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Enzymatic Improvement in the Polyphenol Extractability and Antioxidant Activity of Green Tea Extracts

Cited by 57 publications

References 48 publications

USING CELLULASE TO IMPROVE THE EXTRACTION EFFICIENCY OF THE ANTIOXIDANT COMPOUNDS FROM WHITE RADISH (Raphanus sativus L.) POWDER

USING CELLULASE TO IMPROVE THE EXTRACTION EFFICIENCY OF THE ANTIOXIDANT COMPOUNDS FROM WHITE RADISH (Raphanus sativus L.) POWDER

Improving yield and composition of protein concentrates from green tea residue in an agri-food supply chain: Effect of pre-treatment

Light and electron microscopic study of thyroid follicular cells in adult albino rats following green tea administration

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