Enzymatic in‐capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection

Rzygalinski, Ignacy; Poboży, Ewa; Drewnowska, Renata; Trojanowicz, Marek

doi:10.1002/elps.200700726

Cited by 13 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rzygalinski et al. used an identical bi‐enzymatic system for glucose determination, but in combination with more convenient UV detection . They tested both various injection set‐ups – ZP and AI and various compounds as the second peroxidase substrate.…”

Section: Application Of On‐column Derivatisationmentioning

confidence: 99%

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Glatz

2014

Electrophoresis

View full text Add to dashboard Cite

Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context, CE is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses - using special detection techniques, using sample pre-concentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation. Although it can be performed in different ways depending on where the reaction takes place, this article reviews one of the simplest and at the same time most useful approaches on-capillary derivatisation. Even if in many cases the use of on-capillary derivatisation alone is enough to improve the detection sensitivity, on other occasions it needs to be employed in combination with the other above-mentioned strategies. After a simple discussion of derivatisation in general, special attention is focused on the on-capillary approach and methodologies available for on-capillary reactant mixing. Its applications in various fields are also described.

show abstract

Section: Application Of On‐column Derivatisationmentioning

confidence: 99%

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Glatz

2014

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…Later, Rzygalinski et al studied the bienzymatic system of oxidase/peroxidase in CE with spectrophotometric detection for the determination of oxidase substrates. The use of NADH acting both as a chromogenic agent and as a substrate for peroxidase appears to be the simplest procedure 108. Recently, Xie et al developed an EMMA method to simultaneously determine NAD + and NADH in a single cell 109.…”

Section: On‐line Enzymatic Reactionmentioning

confidence: 99%

Recent developments and applications of EMMA in enzymatic and derivatization reactions

2011

View full text Add to dashboard Cite

This review provides systematic coverage of examples in the field of in-capillary electrophorecially mediated microanalysis (EMMA). The recent developments and applications in the time period up to mid 2011 have been described, as well as relevant older papers. The basic principles and modes of in-capillary assays have been demonstrated. An overview is also given of the various injection, separation and detection modes implemented in combination with EMMA. The review is presented in two parts mainly dealing with (i) enzymatic and (ii) derivatization or chemical reactions. Finally, the future trends of CE in performing and monitoring reactions have been drawn.

show abstract

“…Pobozy and co‐workers 20 compared several procedures of in‐capillary bienzymatic reactions with regard to glucose determination with the use of glucose oxidase and horseradish peroxidase. The procedures discussed include zonal sample introduction, classic plug–plug, and at‐inlet EMMA.…”

Section: In‐capillary Enzymatic Reactions By Emmamentioning

confidence: 99%

Recent developments and applications of EMMA in enzymatic and derivatization reactions

2009

View full text Add to dashboard Cite

show abstract

Enzymatic in‐capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection

Cited by 13 publications

References 42 publications

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Recent developments and applications of EMMA in enzymatic and derivatization reactions

Recent developments and applications of EMMA in enzymatic and derivatization reactions

Contact Info

Product

Resources

About