Enzymatic interesterification of triglyceride with surfactant‐coated lipase in organic media

Abstract

Several surfactant-coated enzymes have been prepared by coating lipases of various origins with a nonionic surfactant, glutamic acid dioleylester ribitol (2C(18)Delta(9)GE). Enzymatic interesterification of tripalmitin with oleic acid using the surfactant-coated lipase was carried out in organic media. The surfactant-coated lipases could effectively catalyze the interesterification of glycerides better than did the powder lipases. A suitable organic solvent was an aliphatic hydrocarbon such as isooctane. The enzymatic activity for the interesterification strongly depended on the origin of the lipase. The surfactant-coated lipase prepared by Mucor javanicus showed the highest enzymatic activity for the interesterification of glycerides, although its powder lipase did not show enzymatic activity. Selective interesterification of glycerides could be performed by adjusting the concentration ratio of oleic acid to tripalmitin in isooctane. Di-substituted glyceride could be selectively produced when the concentration ratio of carboxylic acid to glycerides was 7. (c) 1995 John Wiley & Sons, Inc.

Lipase catalyzed ester synthesis for food processing industries

“…The enzymatic interesterification of tripalmitin with oleic acid using the surfactant coated lipase was carried out in the organic media. The surfactant coated lipases could effectively catalyze the interesterification of the glycerides better than the powdered lipases (Goto et al, 1995).…”

“…When the enzyme load, substrate mole ratio and incubation time increased, the mole percent of capric acid incorporation also increased (Jennings et al, 2000). (Goto et al, 1995).…”

Evaluation of Lipid Modified Lipase for Interesterification and Hydrolysis Reactions in n-Hexane.

“…Enzyme coating by oil soluble surfactants has also improved activity in organic media (Okahata & Ijiro, 1988;Goto et al, 1993Goto et al, , 1995Basheer et al, 1995a). It is thought that the hydrophilic heads of the lipid molecules attach to the lipase surface with the hydrophobic tails arranged on the outer side allowing the modified lipase to disperse in hydrophobic solvents (Okahata & Ijiro, 1988;Basheer et al, 1995a).…”

Kinetic study on the esterification of geraniol and acetic acid in organic solvents using surfactant-coated lipase

“…This is presumably due to the rapid deactivation of PL in an organic solvent. According to Goto et al,14 the of SCL was 100-V max fold higher than that of PL. As stated above, the SCL prepared in this work was insoluble in isooctane which caused a high mass transfer resistance and resulted in a relatively low value of Parti-V max .…”