Enzymatic-microwave assisted extraction and high-performance liquid chromatography–mass spectrometry for the determination of selected veterinary antibiotics in fish and mussel samples

Fernández‐Torres, Rut; López, Miguel Ángel; Consentino, M. Olías; Mochón, M. Callejón; Ramos‐Payán, María

doi:10.1016/j.jpba.2010.12.002

Cited by 61 publications

(22 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yang et al [57] developed a method with EAU using a mixture of acetonitrile and citrate buffer (50:50 v/v) placed in an ultrasound bath for 15 min, repeating this process three times. The extract was then purified by using SPE cartridges in series, SAX (6 mL, 500 mg), and HLB (6 mL, 200 mg); the procedure obtained good recoveries for 14 antibiotics studied in the sediment of Pearl rivers in Guangdong province, China, and was also used by Zhou et al [141], which increased the number of analyzed antibiotics.…”

Section: Sample Preparation Techniquesmentioning

confidence: 99%

Veterinary Antibiotics in the Environment

Botelho¹,

Monteiro²,

LuizTornisielo³

2015

Emerging Pollutants in the Environment - Current and Further Implications

View full text Add to dashboard Cite

In recent years, pharmaceutical pollution in the environment has been a great concern due to the potential effects on the human and animal health. Some of the most used classes such as antibiotics, which are used to prevent and treat bacterial infections and promote the growth of livestock, deserve to be highlighted since their intensive use has contaminated environmental matrices such as soil, water, sediment, plants, and animals with effects on the biota. To better understand the potential ecological risk of antibiotics in environments and to develop management strategies for these substances searching to reach the reduction of these compounds in aquatic systems, one of the most important steps is to determine the environmental concentrations of these compounds in the environments through analytical methods and evaluate their effects on the biota. The goal of this chapter is contribute with information about the effects of these compounds on the biota as well as its environmental behavior and bacterial resistance in additional to the main techniques for samples preparation and quantitative and confirmatory methods for its determination in the environment.

show abstract

Section: Sample Preparation Techniquesmentioning

confidence: 99%

Veterinary Antibiotics in the Environment

Botelho¹,

Monteiro²,

LuizTornisielo³

2015

Emerging Pollutants in the Environment - Current and Further Implications

View full text Add to dashboard Cite

show abstract

“…Numerous countries including the United States, the European Union (EU), Japan, and China have strictly banned the use of CAP in food-producing animals. Unfortunately, illegal uses of CAP in livestock have resulted in its detection in honey, tissues, and shrimp samples (Shen and Jang, 2005;Shen et al, 2006;LoPez et al, 2008;Chen et al, 2011;FernanDez-Torres et al, 2011;YiBar et al, 2011). In the EU, a concentration of 0.3 µg kg -1 has been established as the minimum required performance limit (MRPL) for CAP (EC, 2003).…”

mentioning

confidence: 99%

“…However, this method is not suitable for confirmation of CAP in samples of different origins (imPens et al, 2003;CRLs, 2010). It is therefore important to develop a highly sensitive analytical method for the detection of CAP in different samples (Bogusz et al, 2004;Shen and Jang, 2005;LoPez et al, 2008;ReJTharoVÁ and ReJThar, 2009;Siqueira et al, 2009;AresTa et al, 2010;Barganska et al, 2011;BerenDsen et al, 2011;Chen et al, 2011;FernanDez-Torres et al, 2011;Tian, 2011).…”

mentioning

confidence: 99%

“…Analytical procedures to determine CAP focus on gas chromatography (GC) and liquid chromatography (LC) coupled to mass spectrometric (MS) detector (ImPens et al, 2003;AresTa et al, 2010;BerenDsen et al, 2011;Chen et al, 2011;FernanDez-Torres et al, 2011). In LC-MS practice, it is very important to evaluate the method performance in incurred samples.…”

mentioning

confidence: 99%

“…In LC-MS practice, it is very important to evaluate the method performance in incurred samples. Most of the procedures presented in the literature could be applied only to one or two incurred samples (Shen and Jiang, 2005;Shen et al, 2006;Chen et al, 2011;LoPez et al, 2008;FernanDez-Torres et al, 2011;YiBar et al, 2011). In the present paper, an LC-MS/MS method was developed for chicken meat, liver, bovine milk, and porcine urine matrices.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A LC-MS/MS confirmatory method for determination of chloramphenicol in real samples screened by competitive immunoassay

Tölgyesi¹,

Fekete²,

Sharma³

et al. 2014

Acta Alimentaria

View full text Add to dashboard Cite

A new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to confirm chloramphenicol (CAP) residues in foods of animal origin and in urine samples, which were earlier found positive under the screening analysis, performed by competitive enzyme-linked immunoassay (ELISA) technique. The developed LC-MS/MS method was applied to four non-compliant samples from 2008 to 2012; giving concentrations of CAP residues from 1.18 to 3.68 µg kg -1 . All samples, qualified positive by ELISA, were confirmed with the LC-MS/MS technique and found to be non-compliant. The effectiveness of the confirmatory method was proven by participating in a successful proficiency test in year 2010. Both LC-MS/MS and ELISA methods were validated according to the European Union 2002/657/EC decision. The decision limit of the confirmatory method was determined as 0.02 µg kg -1 for CAP in each validated matrix, while the detection capability of the screening test was 0.15 µg kg -1 .

show abstract