Enzymatic modification of cassava starch by bacterial lipase

Abstract

Enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. An industrial lipase preparation produced by Burkholderia cepacia (lipase PS) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. Reactions performed with palmitic acid by liquid-state and microwave esterification gave a degree of substitution (DS) of 62.08% (DS 1.45) and 42.06% (DS 0.98), respectively. Thermogravimetric analysis showed that onset of decomposition is at a higher temperature (above 600 degrees Celsius) for modified starch than the unmodified starch (280 degrees Celsius). Modified starch showed reduction in alpha-amylase digestibility compared to native starch (76.5-18%). Swelling power lowered for modified starch as esterification renders starch more hydrophobic, making it suitable for biomedical applications as materials for bone fixation and replacements, carriers for controlled release of drugs and bioactive agents. Thus enzymatic esterification is ecofriendly.

Improving Cassava Quality for Poultry Feeding Through Application of Biotechnology

“…Enzymatic hydrolysis of cell wall polysaccharides improves the detoxification of cassava roots [65], hence making it a non-toxic and possible candidate as an energy source in the production of feeds for broiler chickens. The fatty acid functional group of lauric and palmitic acids can be imparted to cassava starch by bacterial and fungal lipases [66,67]. Fermentation with A. niger was shown to increase the hemicellulose and amylopectin contents and metabolizable energy value of unpeeled cassava root meal for birds [68], suggesting that fermenting fungal organisms are able to release digestive enzymes which pre-digest the substrate, hence increasing the availability of nutrients [69].…”

Oxidation of potato starch with different sodium hypochlorite concentrations and its effect on biodegradable films

“…Starch is a renewable raw material, abundant and available in all the world, and shows properties that are modifiable from the physical , chemical (Kuakpetoon & Wang, 2001;Menzel et al, 2013) and enzymatic (LeCorre, Vahanian, Dufresne, & Bras, 2012;Rajan & Abraham, 2006) or combined (Spier, Zavareze, Silva, Elias, & Dias, 2013) ways, which can result in resistant films and coatings (Cyras, Zenklusen, & Vazquez, 2006;Mali, Grossmann, Garcıa, Martino, & Zaritzky, 2004;Zavareze et al, 2012). Some studies about starch films' functional properties characterization have been developed, with the result that the biggest technological problems and application are high water vapor permeability and low mechanical resistance.…”

Synthesis of Rosin Acid Starch Catalyzed by Lipase

“…Besides, they can also catalyze esterification, acidolysis, interesterification, alcoholysis, and aminolysis and have been widely applied in food, dairy, detergent, and pharmaceutical industries [17]. Lipases have been used as effective catalyst for starch esterification [9], and the enzymatic esterification is superior to the conventional chemical-catalyzed one as it works under milder reaction conditions with higher reaction selectivity and fewer by-products.…”