Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties

Volkov, Vadim; Cavaco-Paulo, Artur

doi:10.1016/j.ijbiomac.2015.12.082

Cited by 11 publications

(9 citation statements)

References 64 publications

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“…Several physical and biochemical techniques have already been used to test human hair and characterise keratin fibres, such as x‐ray diffraction, solid state nuclear magnetic resonance (NMR), circular dichroism, scanning electron microscopy and high‐pressure liquid chromatography–mass spectrometry (HPLC–MS) . Although biochemical and microscopic techniques are innovative, they present some technical limitations owing to protocol complexities, prior sample preparation, the need for extraction, and reagent costs.…”

Section: Introductionmentioning

confidence: 99%

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

Essendoubi

Meunier

Scandolera

et al. 2019

Intern J of Cosmetic Sci

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ObjectiveIn hair care cosmetic products’ evaluation, one commonly used method is to evaluate the hair appearance as a gold standard in order to determine the effect of an active ingredient on the final state of the hair via visual appreciation. Although other techniques have been proposed for a direct analysis of the hair fibres, they give only surface or structural information, without any accurate molecular information. A different approach based on confocal Raman spectroscopy has been proposed for tracking in situ the molecular change in the keratin directly in the human hair fibres. It presents a high molecular specificity to detect chemical interactions between molecules and can provide molecular information at various depths at the cortex and cuticle levels.MethodsTo evaluate the potential of confocal Raman spectroscopy in testing the efficiency of cosmetic ingredients on keratin structure, we undertook a pilot study on the effectiveness of a smoothing shampoo on natural human hair, by analysing α‐helix and β‐sheet spectral markers in the Amide I band and spectral markers specific to the cystin sulfur content.ResultsWe confirmed that an active proved to be effective on a gold standard decreases α‐helix keratin conformation and promotes β‐sheet keratin conformation in the hair fibres. We also showed that treatment with the effective active decreases the intensity of covalent disulfide (S–S at 510 cm‐1) cross‐linking bands of cysteine. These data confirm that the effective active also acts on the tertiary structure of keratin.ConclusionFrom these experiments, we concluded that the effective active has a smoothing effect on the human hair fibres by acting on α‐helix and β‐sheet keratin conformation and on the tertiary structure of keratin. Based on these results, confocal Raman spectroscopy can be considered a powerful technique for investigating the influence of hair cosmetic ingredients on keratin structure in human hair fibres. Moreover, this analytical technique has the advantage of being non‐destructive and label free; in addition, it does not require sample extraction or purification and it can be applied routinely in cosmetic laboratories.

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Section: Introductionmentioning

confidence: 99%

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

Essendoubi

Meunier

Scandolera

et al. 2019

Intern J of Cosmetic Sci

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“…As opposed to silk, the keratin (in the solid form of hair tresses) was solely bleached and taken into kinase (Volkov and Cavaco-Paulo 2016). Due to the specificity of its processing, no bioinformatics-based prediction regarding the number of possible phosphosites could be drawn.…”

Section: Hair Keratinmentioning

confidence: 99%

“…The third article reported that for hair keratin, the immediate outcome of phosphorylation is the enhanced affinity of a model cationic compound towards the phosphorylated material, as assessed by adsorption capacity Q measurements of methylene blue (MB) dye (Volkov and Cavaco-Paulo 2016). Since the enzymatic reaction was carried out on pre-formed solid material, only the considerations of adsorbent surface potential and adsorbate-adsorbent interactions were thoroughly studied and presented (Volkov and Cavaco-Paulo 2016).…”

Section: Hair Keratinmentioning

confidence: 99%

“…The chosen results of kinetic pseudo-second order and Langmuir isothermal modelings are presented in Tables 1 and 2, respectively. Several important aspects of adsorption, such as the rate-limiting step of MB removal from a solution and its deposition on hair and thermodynamic modeling outcomes like the mean energy of adsorption, among others, were elucidated (Volkov and Cavaco-Paulo 2016). They made possible to conclude that the pseudo-second order (PSO) model, along with Langmuir isothermal model, characterizes best the hair-based adsorption system.…”

Section: Hair Keratinmentioning

confidence: 99%

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In vitro phosphorylation as tool for modification of silk and keratin fibrous materials

Volkov

Cavaco-Paulo

2016

Appl Microbiol Biotechnol

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An overview is given of the recent work on in vitro enzymatic phosphorylation of silk fibroin and human hair keratin. Opposing to many chemical "conventional" approaches, enzymatic phosphorylation is in fact a mild reaction and the treatment falls within "green chemistry" approach. Silk and keratin are not phosphorylated in vivo, but in vitro. This enzyme-driven modification is a major technological breakthrough. Harsh chemical chemicals are avoided, and mild conditions make enzymatic phosphorylation a real "green chemistry" approach. The current communication presents a novel approach stating that enzyme phosphorylation may be used as a tool to modify the surface charge of biocompatible materials such as keratin and silk.

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“…This shows that α-keratin has the potential to be further developed. α-keratin is capable of acting as an adsorbent because it has a binding amino and hydroxyl functional group which causes the cation polyelectrolyte properties to act as ion exchangers and potentially developed as adsorbents such as for removal of Fe ions [5].…”

Section: Introductionmentioning

confidence: 99%

Adsorption of Fe Ions from Aqueous Solution Using α-Keratin-Coated Alginate Biosorbent

Fadillah¹,

Putri²,

Febrianastuti³

et al. 2018

IJESD

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Abstract-The biosorbent α-keratin-alginate (KA) was prepared by using the encapsulation technique in CaCl 2 2 % (w/v) solution. The biosorbent was characterized by Fourier Transform Infrared (FTIR) and Scanning Electron Microscope (SEM). The extent of adsorption was found to be a function of the composition of α-keratin and alginate, the pH of solution and contact time. The optimum adsorption of Fe ions in aqueous solution was found at the composition of α-keratin and alginate of 1:2 (w/w), the pH at 7.0 and contact time at 60 minutes. The adsorption of Fe ions on KA biosorbent was comparatively higher than α-keratin and alginate only. The adsorption capacity of KA biosorbent has the maximum adsorption capacity of 658.4 mg/g while biosorbent α-keratin and alginate are 464.7 mg/g and 528.1 mg/g, respectively. Adsorption of Fe ions in aqueous solution followed the Freundlich adsorption isotherm, and the dynamic adsorption model could be described through a pseudo-second order kinetics.

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Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties

Cited by 11 publications

References 64 publications

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

In vitro phosphorylation as tool for modification of silk and keratin fibrous materials

Adsorption of Fe Ions from Aqueous Solution Using α-Keratin-Coated Alginate Biosorbent

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