Enzymatic Polymerization of Cyclic Monomers in Ionic Liquids as a Prospective Synthesis Method for Polyesters Used in Drug Delivery Systems

Piotrowska, Urszula; Sobczak, Marcin

doi:10.3390/molecules20010001

Cited by 43 publications

(34 citation statements)

References 131 publications

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“…Similarly, mutations in AIPL1 are already known to be implicated in both ar LCA and ad CRD [72]. A recent study identified two homozygous mutations in AIPL1 (c.773G>C (p.R258P) and c.465G>T (p.(H93_Q155del)) in two Pakistani families, which co-segregated with disease phenotype that could correspond to ar LCA or ar RP [73]. In 1 patient of our cohort, 2 novel likely pathogenic mutations were identified in exon 5 of AIPL1 (het c.769C>T p.(L257F) + het c.767T>G p.(I256S)), one carried by the unaffected father and one by the unaffected mother.…”

Section: Discussionmentioning

confidence: 99%

Next-generation sequencing applied to a large French cone and cone-rod dystrophy cohort: mutation spectrum and new genotype-phenotype correlation

Boulanger-Scemama

Shamieh

Démontant

et al. 2015

Orphanet J Rare Dis

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BackgroundCone and cone-rod dystrophies are clinically and genetically heterogeneous inherited retinal disorders with predominant cone impairment. They should be distinguished from the more common group of rod-cone dystrophies (retinitis pigmentosa) due to their more severe visual prognosis with early central vision loss. The purpose of our study was to document mutation spectrum of a large French cohort of cone and cone-rod dystrophies.MethodsWe applied Next-Generation Sequencing targeting a panel of 123 genes implicated in retinal diseases to 96 patients. A systematic filtering approach was used to identify likely disease causing variants, subsequently confirmed by Sanger sequencing and co-segregation analysis when possible.ResultsOverall, the likely causative mutations were detected in 62.1 % of cases, revealing 33 known and 35 novel mutations. This rate was higher for autosomal dominant (100 %) than autosomal recessive cases (53.8 %). Mutations in ABCA4 and GUCY2D were responsible for 19.2 % and 29.4 % of resolved cases with recessive and dominant inheritance, respectively. Furthermore, unexpected genotype-phenotype correlations were identified, confirming the complexity of inherited retinal disorders with phenotypic overlap between cone-rod dystrophies and other retinal diseases.ConclusionsIn summary, this time-efficient approach allowed mutation detection in the most important cohort of cone-rod dystrophies investigated so far covering the largest number of genes. Association of known gene defects with novel phenotypes and mode of inheritance were established.Electronic supplementary materialThe online version of this article (doi:10.1186/s13023-015-0300-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Next-generation sequencing applied to a large French cone and cone-rod dystrophy cohort: mutation spectrum and new genotype-phenotype correlation

Boulanger-Scemama

Shamieh

Démontant

et al. 2015

Orphanet J Rare Dis

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“…Biodegradable polymeric nanoparticles, which are capable of achieving a variety of sustained release profiles of active substances with unique pharmacokinetics, offer great potential as delivery vehicles. [123] The release of AMPs from the polymeric carriers is controlled by several concurrent mechanisms: diffusion through the nanosphere matrix or nanocapsule polymer wall, erosion of the matrices or by osmotic pressure, which means that the process is very complex. [121] To date, many methods have been developed for the preparation of polymeric nanoparticles (emulsion or interfacial | 1087 PIOTROWSKA eT Al.…”

Section: Polymer-based Ddsmentioning

confidence: 99%

Current state of a dual behaviour of antimicrobial peptides—Therapeutic agents and promising delivery vectors

2017

Self Cite

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Micro-organism resistance is an important challenge in modern medicine due to the global uncontrolled use of antibiotics. Natural and synthetic antimicrobial peptides (AMPs) symbolize a new family of antibiotics, which have stimulated research and clinical interest as new therapeutic options for infections. They represent one of the most promising antimicrobial substances, due to their broad spectrum of biological activity, against bacteria, fungi, protozoa, viruses, yeast and even tumour cells. Besides, being antimicrobial, AMPs have been shown to bind and neutralize bacterial endotoxins, as well as possess immunomodulatory, anti-inflammatory, wound-healing, angiogenic and antitumour properties. In contrast to conventional antibiotics, which have very defined and specific molecular targets, host cationic peptides show varying, complex and very rapid mechanisms of actions that make it difficult to form an effective antimicrobial defence. Importantly, AMPs display their antimicrobial activity at micromolar concentrations or less. To do this, many peptide-based drugs are commercially available for the treatment of numerous diseases, such as hepatitis C, myeloma, skin infections and diabetes. Herein, we present an overview of the general mechanism of AMPs action, along with recent developments regarding carriers of AMPs and their potential applications in medical fields.

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“…The use of enzymes (mainly lipases) in ROP reactions of lactides and other lactones has been actively pursued in organic solvents . When solvents are used in the synthesis of these polyesters, the common organic solvents are toluene and heptane, which are hazardous, toxic and flammable; lactides are poorly soluble in common organic solvents while melted lactides at high temperatures are polar/hydrophilic and inhibitory to the enzymatic propagation . In addition, lipases typically show moderate activities in these media, especially towards l ‐lactide and dl ‐lactide .…”

Section: Enzymatic Rop In Organic Solventsmentioning

confidence: 99%

“…These biodegradable polyesters are thermoplastic, and biocompatible polymers with mechanical properties similar to those of polystyrene or polyethylene terephthalate (PET or PETE) . In addition to their uses in packaging and horticultural materials, polyesters (with an average molecular weight > 60 000 g mol ‐1 ) have advantageous applications in biomedical fields (due to their high biocompatibility and lack of toxicity) including controlled drug delivery carriers (such as for Camptothecin, Doxorubicin, Amphotericin B, and Vancomycin, etc., and for therapeutic peptides/proteins and for gene delivery), tissue engineering scaffolds, surgical suture and bone fixation materials . Aliphatic polyesters can be synthesized by two common methods, i.e.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic ring‐opening polymerization (ROP) of polylactones: roles of non‐aqueous solvents

Zhao

2017

J of Chemical Tech & Biotech

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Aliphatic polyesters such as polylactides (PLAs) and other polylactones are thermoplastic, biodegradable and biocompatible polymers with the potential to replace petro-chemical-based synthetic polymers. A benign route for synthesizing these polyesters is through the enzyme-catalyzed ring-opening polymerization (ROP) reaction; this type of enzymatic process is very sensitive to reaction conditions such as solvents, water content and temperature. This review systematically evaluates the crucial roles of different solvents (such as solvent-free/in bulk, organic solvents, supercritical fluids, ionic liquids, and aqueous biphasic systems) on the degree of polymerization and polydispersity. In general, many studies suggest that hydrophobic organic solvents with minimum water contents lead to efficient enzymatic polymerization and subsequently high molecular weights of polyesters; the selection of solvents is also limited by the reaction temperature, e.g. the ROP of lactide is often conducted at above 100 ∘ C, therefore, the solvent typically needs to have its boiling point above this temperature. The use of supercritical fluids could be limited by its scaling-up potential, while ionic liquids have exhibited many advantages including their low-volatility, high thermal stability, controllable enzyme-compatibility, and a wide range of choices. However, the fundamental and mechanistic understanding of the specific roles of ionic liquids in enzymatic ROP reactions is still lacking. Furthermore, the lipase specificity towards L-and D-lactide is also surveyed, followed by the discussion of engineered lipases with improved enantioselectivity and thermal stability. In addition, the preparation of polyester-derived materials such as polyester-grafted cellulose by the enzymatic ROP method is briefly reviewed.

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Enzymatic Polymerization of Cyclic Monomers in Ionic Liquids as a Prospective Synthesis Method for Polyesters Used in Drug Delivery Systems

Cited by 43 publications

References 131 publications

Next-generation sequencing applied to a large French cone and cone-rod dystrophy cohort: mutation spectrum and new genotype-phenotype correlation

Next-generation sequencing applied to a large French cone and cone-rod dystrophy cohort: mutation spectrum and new genotype-phenotype correlation

Current state of a dual behaviour of antimicrobial peptides—Therapeutic agents and promising delivery vectors

Enzymatic ring‐opening polymerization (ROP) of polylactones: roles of non‐aqueous solvents

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