Enzymatic Potential of Native Fungal Strains of Agave Residues

Contreras-Hernández, M.G.; Aréchiga-Carvajal, Elva Teresa; Moreno‐Jiménez, Martha Rocío; González‐Herrera, Silvia Marina; López-Miranda, Javier; Prado-Barragán, Lilia Arely; Rutiaga-Quiñones, Olga Miriam

doi:10.15376/biores.13.1.569-585

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…One disk (5 mm diameter, 24 h incubation) per isolated fungus was taken and seeded on agar plates with the medium described by Contreras-Hernández et al [33]. For each strain, two plates of each medium were inoculated with different carbon sources.…”

Section: Screening Of Enzymatic Productionmentioning

confidence: 99%

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Núñez‐García,

Martínez‐Ávila,

González‐Herrera

et al. 2024

J Basic Microbiol

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Endophytic microbial communities colonize plants growing under various abiotic stress conditions. Candelilla (Euphorbia antisyphilitica Zucc.) is a shrub that develops functionally in arid and semi‐arid zones of Mexico; these conditions generate an association between the plant and the microorganisms, contributing to the production of enzymes as a defense mechanism for resistance to abiotic stress. The objective of this research was to isolate and identify endophyte fungi of candelilla and bioprospection of these endophytic fungi for enzyme production using candelilla by‐products. Fungi were isolated and identified using ITS1/ITS4 sequencing. Their potency index (PI) was evaluated in producing endoglucanase, xylanase, amylase, and laccase. Fermentation was carried out at 30°C for 8 days at 200 rpm, with measurements every 2 days, using candelilla by‐products as substrate. All fungi exhibited higher cellulase, amylase, and laccase activities on the 2nd, 6th, and 8th day of fermentation, respectively, of fermentation. The fungus Aspergillus niger ITD‐IN4.1 showed the highest amylase activity (246.84 U/mg), the genus Neurospora showed the highest cellulase activity, reaching up to 13.45 FPU/mg, and the strain Neurospora sp. ITD‐IN5.2 showed the highest laccase activity (3.46 U/mg). This work provides the first report on the endophytic diversity of E. antisyphilitica and its potential role in enzyme production.

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Section: Screening Of Enzymatic Productionmentioning

confidence: 99%

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Núñez‐García,

Martínez‐Ávila,

González‐Herrera

et al. 2024

J Basic Microbiol

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“…We have also reported that these cellulose nanoplatelets are easier to hydrolyze than larger cellulose, due to their low lignin content, poor crystallinity, and greater surface area [13]. Also, several strains of enzyme-producer fungi have been isolated from Agave species [17,18]. For instance, in earlier studies, we evaluated the enzymatic activity of different fungal strains previously isolated from A. salmiana leaves, with the highest enzymatic activity resulting from a fungus preliminarily classified as belonging to the genus Penicillium (unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Agave Leaves as a Substrate for the Production of Cellulases by Penicillium sp. and the Obtainment of Reducing Sugars

Silva-Mendoza

Gómez-Treviño

López-Chuken

et al. 2020

Journal of Chemistry

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Lignocellulosic biomass can be used to obtain fermentable sugars by enzymatic hydrolysis, and also it serves as a carbon source to produce cellulases by solid-state fermentation. In this study, we propose the use of leaves of Agave salmiana as a carbon source to produce cellulases by the fungus Penicillium sp., isolated from the same plant. The crude enzymatic extract was used to obtain sugars from the hydrolysis of the parenchymal cells of the leaves. The enzymes produced were characterized (endoglucanase 14.4 U/g; exoglucanase 3.5 U/g; β-glucosidase 4.14 U/g). The enzymes showed activities at elevated temperatures: 50°C for endoglucanase and exoglucanase and 70°C for β-glucosidase. Furthermore, the crude enzymatic extract obtained was able to hydrolyze the parenchyma in 51.6% in 48 h. The evidence presented in this paper shows the potential of the agave leaves as a source of carbon in the production of enzymes by fermentation with the consequent production of reducing sugars. In addition, the enzymes produced by Penicillium sp. could be used in the production of bioethanol, since they work at high temperatures.

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Bioconversion of Agave Bagasse to Produce Cellulases and Xylanases by Penicillium citrinum and Aspergillus fumigatus in Solid-State Fermentation

Valle-Pérez

Flores-Cosío

Amaya-Delgado

2021

Waste Biomass Valor

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Enzymatic Potential of Native Fungal Strains of Agave Residues

Cited by 3 publications

References 43 publications

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Agave Leaves as a Substrate for the Production of Cellulases by Penicillium sp. and the Obtainment of Reducing Sugars

Bioconversion of Agave Bagasse to Produce Cellulases and Xylanases by Penicillium citrinum and Aspergillus fumigatus in Solid-State Fermentation

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