Enzymatic production of isoamyl acetate in an ionic liquid–alcohol biphasic system

Fehér, Erika; Illeová, Viera; Kelemen-Horváth, I.; Bélafi–Bakó, Katalin; Polakovič, Milan; Gubicza, L.

doi:10.1016/j.molcatb.2007.09.019

Cited by 57 publications

(25 citation statements)

References 30 publications

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“…Similar results were reported in the literature, where the biocatalyst had to be replaced after the regeneration due to its denaturation [9,36].…”

Section: Regeneration Of Ionic Liquidsupporting

confidence: 89%

“…Recyclability of ionic liquid together with the immobilized CaLB (Novozym 435) in isoamyl acetate synthesis from acetic acid and isoamyl alcohol was tested by Feher et al [36], and their results revealed the same level of lipase B activity within 10 cycles. Pavlidis et al [14] also developed a simple procedure suitable for ester separation and enzyme reuse in a batch process.…”

Section: Biotransformation With Reused Enzyme and Ionic Liquidmentioning

confidence: 99%

“…Because ionic liquids are still very expensive, processes involving these solvents are competitive only if the ionic liquid phase is recycled and reused as many times as possible [22]. Such protocols were already tested in several batch processes [14,22,23,35,36], while, to the best of our knowledge, the reuse of solvents within microfluidic devices employing biocatalysts has not yet been described.…”

Section: Introductionmentioning

confidence: 99%

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Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle

Novak

Žnidaršič–Plazl

2013

Green Processing and Synthesis

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Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets of n-heptane within a continuous phase of ionic liquid with dissolved enzyme and reactants, namely, isoamyl alcohol and acetic anhydride. A developed flow pattern resulted in a very large specific interfacial area for the reaction with amphiphilic CaLB and simultaneous extraction of isoamyl acetate in n-heptane. Biotransformation was performed within a continuously operated microfluidic system consisting of an X-junction chip and a silanized tube of a submillimeter diameter, which was further integrated with a microseparator based on a hydrophobic membrane. Efficient separation of n-heptane with product from ionic liquid phase containing enzyme and remaining substrates and products was achieved, enabling reuse of CaLB together with ionic liquid phase. More than 80% of productivity was preserved in each of the eight consecutive biotransformations within the integrated microfluidic system with reused lipase B in the ionic liquid phase. Subsequent ionic liquid regeneration accomplished by vacuum distillation enabled efficient reuse of this solvent in esterification with fresh enzyme.

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“…Similar results were reported in the literature, where the biocatalyst had to be replaced after the regeneration due to its denaturation [9,36].…”

Section: Regeneration Of Ionic Liquidsupporting

confidence: 89%

Section: Biotransformation With Reused Enzyme and Ionic Liquidmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle

Novak

Žnidaršič–Plazl

2013

Green Processing and Synthesis

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“…The synthesis of isoamyl acetate with Novozym 435 suspended in IL and isoamyl alcohol/acetic acid/toluene/ water mixture as the non-reactive phase was compared for six IL of which three are non-miscible with the non-reactive phase (Fehér et al 2008 [PF 6 ] of which the first was chosen for cost considerations. The reactive phase was reused seven times without apparent loss of activity; however, 21% mass loss of IL is reported.…”

Section: Introductionmentioning

confidence: 99%

Ionic liquids in biotechnology: applications and perspectives for biotransformations

Roosen

Müller

Greiner

2008

Appl Microbiol Biotechnol

163

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Ionic liquids are considered as an alternative to organic solvents for catalysis. The literature in this field is reviewed with focus on advantageous use of ionic liquids in biocatalysis and biotransformations. The overview reveals that the exploration and mapping of ionic liquids with respect to biocatalysis is still sketchy. It is apparent that advantages can be gained in view of activity, stability and selectivity. Furthermore, integration of reaction and separation has a high potential in the field. The review presents quantitative data on the productivities, space-time yields, as well as stability as far as they can be extracted from the literature.

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“…In addition, biphasic systems based on ionic liquid-water or ionic liquid-organic solvent have been assayed for biocatalytic processes [35,36]. For example, the lipase catalyzed kinetic resolution of rac-ibuprofen in a water -ionic liquids biphasic medium [37], lipase catalyzed production of isoamyl acetate in an ionic liquid-alcohol biphasic system [38], and lipase catalyzed kinetic resolution of rac-1-phenylethanol in both ionic liquid/ hexane [39]. In these biphasic reaction systems, the products can be easily recovered by liquid-liquid extraction.…”

Section: Enzymes In Ionic Liquidsmentioning

confidence: 99%

Compatibility of Ionic Liquids with Enzymes

Lan

Koo

2013

Production of Biofuels and Chemicals With Ionic Liquids

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The potential of ionic liquids as a green alternative to environmentally harmful volatile organic solvents has been well recognized. Being considered as "designer solvents", ionic liquids have been used extensively in a wide range of applications including biotransformations. As compared to those in traditional organic solvents, enzyme performance in ionic liquids is showed enhance in their activity, enantioselectivity, stability, as well as their recoverability and recyclability. This chapter will cover the biocompatibility issue of ionic liquids with enzymes. The effects of ionic liquid properties on the enzymatic reactions and conformation of enzyme as well as methods for activation and stabilization of enzymes in ionic liquids will be described. In addition, the current attempts for rational design of biocompatible ionic liquids will be also discussed.

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Enzymatic production of isoamyl acetate in an ionic liquid–alcohol biphasic system

Cited by 57 publications

References 30 publications

Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle

Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle

Ionic liquids in biotechnology: applications and perspectives for biotransformations

Compatibility of Ionic Liquids with Enzymes

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