Enzymatic Reaction Generates Biomimic Nanominerals with Superior Bioactivity

Jiang, Yingying; Zhou, Zifei; Zhu, Ying‐Jie; Chen, Feifei; Lu, Bing‐Qiang; Cao, Weiguo; Zhang, Yonggang; Cai, Zhengdong; Chen, Feng

doi:10.1002/smll.201804321

Cited by 22 publications

(26 citation statements)

References 60 publications

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“…We think that the activation of platelets is related to the large amount of ADP molecules in EACPNs. In our previous study, we revealed the content of ADP molecules in EACPNs by a high-performance liquid chromatography (HPLC) method, which indicated that there is approximately 0.15 g of ADP molecules in each gram of EACPNs ( Jiang et al, 2018 ). It has been reported that ADP, as a soluble agonist released by activated cells, can trigger platelet activation through GPCRs, thereby promoting coagulation formation ( Yun et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

“…EACPNs were prepared via an enzymatic reaction strategy reported in our previous work ( Jiang et al, 2018 ). Briefly, CaCl 2 (66.0 mg) was dissolved into deionized water (30.0 ml), and then aqueous solution (30.0 ml) containing Na 2 ATP (110.0 mg) was added drop-wise into the above Ca 2+ solution under magnetic stirring.…”

Section: Methodsmentioning

confidence: 99%

“…Through the understanding of the bone formation process, we think that the combined use of calcium phosphate-based biominerals with natural constituents in bone regeneration may provide a new strategy to design functional biomaterials. Our previous work reported an enzymatic strategy to synthesize biominerals of amorphous calcium phosphate nanoparticles (EACPNs), which can promote the proliferation and osteogenic differentiation of hBMSCs ( Jiang et al, 2018 ). The EACPNs may affect the activation of platelets and further lead to an acceleration of bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Platelet-Activating Biominerals Enhanced Injectable Hydrogels With Superior Bioactivity for Bone Regeneration

Chen

Yan

Jiang

et al. 2022

Front. Bioeng. Biotechnol.

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Refractory bone fracture, which is difficult to be treated, is a common clinical disease. Taking inspiration from the natural process of bone regeneration, we provide a biomimetic strategy to develop a new injectable biomaterial for repairing bone defects, which is mainly composed of platelets, fibrins, and biominerals. Biomineral nanoparticles (EACPNs) with an amorphous phase are prepared by an enzyme-catalyzed route and display a platelet-activating property. The composite hydrogel (EPH) of EACPNs, fibrins, and platelets is injectable, and has similar chemical properties to natural materials in bone regeneration. The dried EPH samples display a highly porous structure, which would be favorable for cell attachment and growth. The results from in vitro studies indicate that EPH has high biocompatibility and superior bioactivity in promoting the osteogenic differentiation of rat bone marrow stem cells (rBMSCs). Furthermore, the results from in vivo studies clearly indicate that EPH can induce the formation of new collagen and vessels in the defect area, thus leading to faster regeneration of bone defects at 2 weeks. Our study provides a strategy for designing new biomimetic materials, which may be favorable in the treatment of refractory bone fracture.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet-Activating Biominerals Enhanced Injectable Hydrogels With Superior Bioactivity for Bone Regeneration

Chen

Yan

Jiang

et al. 2022

Front. Bioeng. Biotechnol.

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“…Amorphous calcium phosphate (ACP) plays an important role in the biomineralization of bone, teeth, and other tissues, serving as a precursor for apatite minerals [ [1] , [2] , [3] , [4] , [5] ]. Previous studies revealed that ACP was found intracellularly in mineralizing cells, especially as mineral granules in mitochondria [ 6 , 7 ], which can be transported in matrix vesicles via lysosomes and secreted by exocytosis to the extracellular matrix for biomineralization [ 8 ]. The C-terminal end of collagen molecules with a positive net charge promoted the infiltration of these ACP granules to collagen fibrils [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

Cui

Cong

et al. 2021

Bioactive Materials

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Amorphous calcium phosphate (ACP) has been widely found during bone and tooth biomineralization, but the meta-stability and labile nature limit further biomedical applications. The present study found that the chelation of polyacrylic acid (PAA) molecules with Ca 2+ ions in Mg-ACP clusters (~2.1 ± 0.5 nm) using a biomineralization strategy produced inorganic-organic Mg-ACP/PAA hybrid nanoparticles with better thermal stability. Mg-ACP/PAA hybrid nanoparticles (~24.0 ± 4.8 nm) were pH-responsive and could be efficiently digested under weak acidic conditions (pH 5.0–5.5). The internalization of assembled Mg-ACP/PAA nanoparticles by MC3T3-E1 cells occurred through endocytosis, indicated by laser scanning confocal microscopy and cryo-soft X-ray tomography. Our results showed that cellular lipid membranes remained intact without pore formation after Mg-ACP/PAA particle penetration. The assembled Mg-ACP/PAA particles could be digested in cell lysosomes within 24 h under weak acidic conditions, thereby indicating the potential to efficiently deliver encapsulated functional molecules. Both the in vitro and in vivo results preliminarily demonstrated good biosafety of the inorganic-organic Mg-ACP/PAA hybrid nanoparticles, which may have potential for biomedical applications.

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“…Synthetic CaP-based biomaterials have demonstrated excellent biodegradability, bioactivity and osteoconductivity, and are widely used for bone-defect repair and prevention of bone-loss diseases (e.g. osteoporosis) [ 8–12 ]. The detailed composition of CaP in natural bones is far more complicated, since it usually contains other ions that substitute the original CaP structure such as

− , Mg 2+ and Na + [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Amorphous calcium magnesium phosphate nanocomposites with superior osteogenic activity for bone regeneration

Jiang

Tan

et al. 2021

Regenerative Biomaterials

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The seek of bioactive materials for promoting bone regeneration is a challenging and long-term task. Functionalization with inorganic metal ions or drug molecules are considered effective strategies to improve the bioactivity of various existing biomaterials. Herein, amorphous calcium magnesium phosphate (ACMP) nanoparticles and simvastatin (SIM)-loaded ACMP (ACMP/SIM) nanocomposites were developed via a simple coprecipitation strategy. The physiochemical property of ACMP/SIM were explored using transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD) and high performance liquid chromatograph (HPLC), and the role of Mg2+ in the formation of ACMP/SIM was revealed using X-ray absorption near-edge structure (XANES). After that, the transformation process of ACMP/SIM in simulated body fluid (SBF) was also tracked to simulate and explore the in vivo mineralization performance of materials. We find that ACMP/SIM releases ions of Ca2+, Mg2+ and PO43-, when it is immersed in SBF at 37 °C, and a phase transformation occured during which the initially amorphous ACMP turns into self-assembled hydroxyapatite (HAP). Furthermore, ACMP/SIM displays high cytocompatibility and promotes the proliferation and osteogenic differentiation of MC3T3-E1 cells. For the in vivo studies, lamellar ACMP/SIM/Collagen scaffolds with aligned pore structures were prepared and used to repair a rat defect model in calvaria. ACMP/SIM/Collagen scaffolds show a positive effect in promoting the regeneration of calvaria defect after 12 weeks. The bioactive ACMP/SIM nanocomposites are promising as bone repair materials. Considering the facile preparation process and superior in vitro/vivo bioactivity, the as-prepared ACMP/SIM would be a potential candidate for bone related biomedical applications.

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Enzymatic Reaction Generates Biomimic Nanominerals with Superior Bioactivity

Cited by 22 publications

References 60 publications

Platelet-Activating Biominerals Enhanced Injectable Hydrogels With Superior Bioactivity for Bone Regeneration

Platelet-Activating Biominerals Enhanced Injectable Hydrogels With Superior Bioactivity for Bone Regeneration

Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

Amorphous calcium magnesium phosphate nanocomposites with superior osteogenic activity for bone regeneration

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