Enzymatic recognition of DNA damage induced by UVB-photosensitized titanium dioxide and biological consequences in Saccharomyces cerevisiae: Evidence for oxidatively DNA damage generation

Pinto, Alicia Viviana; Deodato, Elder L.; Cardoso, Janine Simas; Oliveira, Eliza F.; Machado, Sandro Lemos; Toma, Helena Keiko; Leitão, Álvaro C.; Pádula, Marcelo de

doi:10.1016/j.mrfmmm.2010.02.003

Cited by 24 publications

(16 citation statements)

References 65 publications

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“…The BMNC from NHD13 mice showed a 3-fold increase in frameshift mutations compared to BMNC from WT mice. These findings are consistent with reports of increased frameshift mutations associated with increased ROS [12, 45]. Moreover, several reports have linked increased levels of ROS with single nucleotide insertions or deletions at mono or di-nucleotide repeat sequences [46–48].…”

Section: Discussionsupporting

confidence: 92%

Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome

et al. 2014

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The myelodysplastic syndromes (MDS) are characterized by ineffective hematopoiesis, dysplasia, and transformation to acute myeloid leukemia (AML). Although it has been suggested that additional mutations lead to progression of MDS to AML, the causative agent(s) for such mutations remains unclear. Oxidative stress is a potential cause, therefore, we evaluated levels of reactive oxygen species (ROS) in NUP98-HOXD13 (NHD13) transgenic mice, a murine model for MDS. Increased levels of ROS were detected in bone marrow nucleated cells (BMNC) that express CD71, a marker for cell proliferation, as well as immature, lineage negative bone marrow nucleated cells from NHD13 mice. In addition to the increase in ROS, increased DNA double strand breaks and activation of a G2/M phase cell cycle checkpoint were noted in NHD13 BMNC. Finally, using an in vivo assay for mutation frequency, we detected an increased mutation frequency in NHD13 BMNC. These results suggest that oxidative stress may contribute to disease progression of MDS to AML through ineffective repair of DNA damage and acquisition of oncogenic mutations.

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Section: Discussionsupporting

confidence: 92%

Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome

et al. 2014

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“…However, the lack of increase in the level of strand breaks and Endo III-sensitive sites would argue in favor of a significant contribution of 1 O 2 in the TiO 2 -photosensitized generation of oxidatively generated damage rather than of • OH. One may also quote a recent study in which preincubation of S. cerevisiae cells with normal size TiO 2 (>100 nm) led to an increase in mutation frequencies in both wild-type and 8-oxoguanine DNA N-glycosylase strains upon UVB irradiation (203). This that was partly prevented by the presence of metal chelators such as dipyridyl and neocuprine is suggestive of the predominant role of…”

Section: Oxidation Reactions Mediated By Photosensitizersmentioning

confidence: 93%

Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions^†

Cadet¹,

Mouret²,

Ravanat³

et al. 2012

Photochem & Photobiology

270

251

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The survey focuses on recent aspects of photochemical reactions to cellular DNA that are implicated through the predominant formation of mostly bipyrimidine photoproducts in deleterious effects of human exposure to sunlight. Recent developments in analytical methods have allowed accurate and quantitative measurements of the main DNA photoproducts in cells and human skin. Highly mutagenic CC and CT bipyrimidine photoproducts, including cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) are generated in low yields with respect to TT and TC photoproducts. Another striking finding deals with the formation of Dewar valence isomers, the third class of bipyrimidine photoproducts that is accounted for by UVA-mediated isomerization of initially UVB generated 6-4PPs. Cyclobutadithymine (T< >T) has been unambiguously shown to be involved in the genotoxicity of UVA radiation. Thus, T< >T is formed in UVA-irradiated cellular DNA according to a direct excitation mechanism with a higher efficiency than oxidatively generated DNA damage that arises mostly through the Type II photosensitization mechanism. C<>C and C< >T are repaired at rates intermediate between those of T< >T and 6-4TT. Evidence has been also provided for the occurrence of photosensitized reactions mediated by exogenous agents that act either in an independent way or through photodynamic effects.

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“…These were established as the maximal nontoxic concentrations of MMT and TiO 2 when yeast cells were incubated with MMT or TiO 2 under agitation in the dark, as both spontaneous mutagenesis and cell viability were not modified. In addition, higher MMT or TiO 2 concentrations (especially when in a mixture) tend to produce sedimentation (data not shown). Treatments were: (1) UVB, (2) UVB + TiO 2 , (3) UVB + MMT, (4) UVB + TiO 2 + MMT, and (v) UVB + TiO 2 –MMT NC in the presence or absence of ODP.…”

Section: Methodsmentioning

confidence: 93%

“…Treatments were: (1) UVB, (2) UVB + TiO 2 , (3) UVB + MMT, (4) UVB + TiO 2 + MMT, and (v) UVB + TiO 2 –MMT NC in the presence or absence of ODP. Cell irradiation with UVB was performed using a 15‐W Vilber Lourmart Ultraviolet lamp as previously described . After each UVB dose, aliquots were taken, properly diluted in SUW, and plated on YPD medium.…”

Section: Methodsmentioning

confidence: 99%

Titanium Dioxide–Montmorillonite Nanocomposite as Photoprotective Agent Against Ultraviolet B Radiation-Induced Mutagenesis in Saccharomyces cerevisiae: A Potential Candidate for Safer Sunscreens

Paiva

Santos

Kibwila

et al. 2014

Journal of Pharmaceutical Sciences

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Enzymatic recognition of DNA damage induced by UVB-photosensitized titanium dioxide and biological consequences in Saccharomyces cerevisiae: Evidence for oxidatively DNA damage generation

Cited by 24 publications

References 65 publications

Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome

Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome

Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions^†

Titanium Dioxide–Montmorillonite Nanocomposite as Photoprotective Agent Against Ultraviolet B Radiation-Induced Mutagenesis in Saccharomyces cerevisiae: A Potential Candidate for Safer Sunscreens

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Enzymatic recognition of DNA damage induced by UVB-photosensitized titanium dioxide and biological consequences in Saccharomyces cerevisiae: Evidence for oxidatively DNA damage generation

Cited by 24 publications

References 65 publications

Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome

Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome

Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions†

Titanium Dioxide–Montmorillonite Nanocomposite as Photoprotective Agent Against Ultraviolet B Radiation-Induced Mutagenesis in Saccharomyces cerevisiae: A Potential Candidate for Safer Sunscreens

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Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions^†