Enzymatic Relationships in the Utilization of Soybean Oil Meal Phosphorus by the Rat

Spitzer, Robert R.; Phillips, Paul H.

doi:10.1093/jn/30.3.183

Cited by 36 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chick exhibits a marked difference from the rat and several other mammalian species in its inability to utilize phytin phosphorus with any degree of efficiency even in the presence of adequate vitamin D. A number of workers (Krieger et al 1940, Spitzer et al 1945 have reported that the rat makes effective use of phytic acid and phytin phosphorus when supplied adequate vitamin D, although this form is less efficient than inorganic phosphorus. The reason for this species difference is by no means clear, but undoubtedly derives from the digestive or absorptive peculiarities of birds.…”

Section: Discussionmentioning

confidence: 99%

“…Singsen and Mitchell (1944) suggested that phytin phosphorus is efficiently utilized by chicks when the ration contains a sufficient amount of the phytin splitting enzyme, phytase. This theory appears to be untenable, however, in view of the results of McGinnis (1944) and Spitzer, et al (1945and Spitzer, et al ( ,1948 who have shown that phytin utilization is not affected by the presence or absence of phytase in the diet, and that this enzyme is naturally present in the digestive tract of animals. Singsen, Matterson, and Scott (1947) have reported that in the case of turkey poults the phytin phosphorus in cereals is not efficiently utilized although vitamin D increases utilization.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Effect of Phytin on the Phosphorus Requirement of the Chick

1949

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The Effect of Phytin on the Phosphorus Requirement of the Chick

1949

View full text Add to dashboard Cite

“…Phytases ( myo ‐inositol hexaphosphate hydrolases) are capable of releasing the phosphate groups from phytate to yield P i , which is then available for absorption and utilization by the animal when this reaction occurs in the digestive tract. Intestinal mucosa preparations from agriculturally important monogastric species such as pigs Sus scrofa (Spitzer & Phillips 1945; Pointillart et al. 1984, 1985; Hu et al.…”

Section: Introductionmentioning

confidence: 99%

The effect of exogenously administered recombinant bovine somatotropin on intestinal phytase activity andin vivophytate hydrolysis in hybrid striped bassMorone chrysops × M. saxatilis

Ellestad¹,

Dahl²,

Angel³

et al. 2003

Aquaculture Nutrition

View full text Add to dashboard Cite

It has been recently demonstrated that hybrid striped bass Morone chrysops · M. saxatilis exhibit intestinal phytase activity, an enzyme capable of liberating inorganic orthophosphate from the phytate molecule, thereby making phytate phosphorus (PP) available for absorption and utilization by the fish. However, it was also determined that hybrid bass were able to digest only 1-2% of dietary PP. Therefore, an experiment was conducted to determine if exogenously administered recombinant bovine somatotropin (rbST) would increase intestinal phytase activity in hybrid striped bass to a level that would improve PP availability. The results indicate that exogenous rbST treatment, administered either through intraperitoneal injection or orally, does increase intestinal brush border membrane protein concentration. While injection with rbST led to significant increases in both specific brush border membrane and total intestinal phytase activity, oral rbST administration did not cause similar increases. Total P and PP digestibility were not influenced by rbST administration. While treatment with rbST does have the potential to enhance intestinal phytase activity in hybrid striped bass, it does not appear to increase PP digestibility. This observation limits the hormone's utility as an effective technique for improving PP bioavailability to this species. KEY WORDS KEY WORDS: acid phosphatase, hybrid striped bass Morone chrysops · M. saxatilis, intestinal phytase, maltase, phytate phosphorus digestibility, somatotropin

show abstract

“…Bruce and Callow ( 3 ) are of the opinion that hydrolysis of phytic acid to inositol and phosphoric acid is a necessary step for the absorption of phosphorus and that this is accomplished by phytase present either in the food or in the gastro-intestinal tract. Phytase has been shown t o be present in the intestine of rats (17). An acid phytase, possibly bacterial in origin, has been shown to be present in the human feces (8).…”

mentioning

confidence: 99%

Studies on the Effect of Germination on the Phosphorus Values of Some Common Indian Pulses

Belatady

Banerjee

1953

Journal of Food Science

View full text Add to dashboard Cite

12) observed that phytic acid phosphorus is nutritionally less available than inorganic phosphorus. Bruce and Callow ( 3 ) are of the opinion that hydrolysis of phytic acid to inositol and phosphoric acid is a necessary step for the absorption of phosphorus and that this is accomplished by phytase present either in the food or in the gastro-intestinal tract. Phytase has been shown t o be present in the intestine of rats (17). An acid phytase, possibly bacterial in origin, has been shown to be present in the human feces (8). Nicolaysan and Njaa (16) showed a distinct depression of calcium absorption in rats and pigs b y diets rich in phytic acid. Courtois and Valentino (9) and Boutwell, Geyer, Halverson, and Hart (2), however, observed that the presence or absence of phytase in food had no effect on the availability of phytin phosphorus. Boutwell et al.( 2 ) further observed that vitamin D makes phytin phosphorus in wheat bran completely available to rats. Pulses are rich in phytin. It was of interest to ascertain whether the phytin content and water soluble inorganic phosphorus content change during the process of germination. The present paper presents the results of such a study carried out with 8 species of pulses. METHODOLOGYThe terms used in this paper are those which are defined by specified analytical procedures. Water soluble inorganic phosphorus is that which is extracted as phosphate by water alone and determined colorirnetrically by the method of Fiske and Subbarow (10). Phytin phosphorus was determined by extracting the phytin with N/2 hydrochloric acid, precipitation thereof as the ferric salt, and determining the phosphate liberated by digesting the precipitate with sulfuric and perchloric acids. This method is that described by RIcCance and Widdowson (12). Total phosphorus is that which is determined as phosphate after digestion of the whole dry plant material with sulfuric and perchloric acids. E X P E R I M E N T A L Germination of the seedlings. One g. of healthy pulse was weighed and kept in dry clean petri dishes; 2 cc. of redistilled water was added on the first day and 0.5 to 1 cc. water was added from the second day. The dishes were kept away from direct sunlight.Estimation of total phosphorus. One g. of pure dry seeds was digested with 20 cc. of sulfuric acid and perchloric acid mixture (10 : 1). The digest was diluted to 100 cc.; 5 cc. aliquot was neutralized, 0.12 cc. of 10 N. sulfuric acid was added and the volume made up to 25 cc. Total phosphorus was estimated in the solution by the method of Fiske and Subbarow (10).Estimation of phytin phosphorus and water soluble inorganic phosphorus. One g. of powdered dried pulse was crushed with water and made up to 2.5 cc. F i r e cc. of this extract mas used for the estimation of water soluble inorganic phosphorus. Concen- 223

show abstract

Enzymatic Relationships in the Utilization of Soybean Oil Meal Phosphorus by the Rat

Cited by 36 publications

References 9 publications

The Effect of Phytin on the Phosphorus Requirement of the Chick

The Effect of Phytin on the Phosphorus Requirement of the Chick

The effect of exogenously administered recombinant bovine somatotropin on intestinal phytase activity andin vivophytate hydrolysis in hybrid striped bassMorone chrysops × M. saxatilis

Studies on the Effect of Germination on the Phosphorus Values of Some Common Indian Pulses

Contact Info

Product

Resources

About