1995
DOI: 10.1097/00007890-199510000-00014
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Enzymatic Removal of Alpha-Galactosyl Epitopes From Porcine Endothelial Cells Diminishes the Cytotoxic Effect of Natural Antibodies

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Cited by 30 publications
(30 citation statements)
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“…Understanding of the critical role of a cell surface disaccharide moiety, gal-a1-3-gal, that binds natural antibodies and 314 induces subsequent activation of the complement system, has led to significant advances in controlling the humoral immune responses against porcine xenografts (4)(5)(6)(7)(8)(9). However, the mechanisms for the vigorous cellular immune response to xenoantigens are still unclear.…”
Section: Introductionmentioning
confidence: 99%
“…Understanding of the critical role of a cell surface disaccharide moiety, gal-a1-3-gal, that binds natural antibodies and 314 induces subsequent activation of the complement system, has led to significant advances in controlling the humoral immune responses against porcine xenografts (4)(5)(6)(7)(8)(9). However, the mechanisms for the vigorous cellular immune response to xenoantigens are still unclear.…”
Section: Introductionmentioning
confidence: 99%
“…In the case of biotherapeutics, the important NSDs are UDP-GlcNAc, UDP-Gal, GDP-Fuc CMP-NeuAc and CMP-NeuGc as well as UDP-GalNAc when murine cell lines are employed over CHO cells. High levels of CMP-NeuGc terminating carbohydrate moieties are unfavorable in a therapeutic context as they are oncofetal and potentially immunogenic [163,164]. The metabolism pathways and biosynthesis of sugars in mammalian cells are well known and are graphically represented in Figure 5 where the transport into the Golgi is also indicated.…”
Section: Medium Formulationmentioning
confidence: 99%
“…Second, intravenous infusion of soluble aGa1 terminated oligosaccharides functioning as haptens in baboons transplanted with pig xenografts [30,39]. Third, change of the expression of Galal-3Gal determinants in the graft by al-3galactosyltransferase gene knockout [32,341, diverging the Galal-3Gal biosynthesis by addition of the al,2fucosyltransferase gene forming the blood group H determinant [5,6,28] or removing the antigen determinant by a-Galactosidase [15,21,361. Fourth, blocking complement activation by infusion of human soluble complement receptor 1 [23] or production of pig strains transgenic for human complement regulatory proteins [9,11,14].…”
Section: Introductionmentioning
confidence: 99%