Enzymatic removal of selected aromatic contaminants from wastewater by a fungal peroxidase from <i>Coprinus macrorhizus</i> in batch reactors

Al-Kassim, Loola; Taylor, Keith E.; Nicell, Jim A.; Bewtra, Jatinder K.; Biswas, Nihar

doi:10.1002/jctb.280610214

Cited by 63 publications

(22 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is a convenient characteristic for the enzymes when they are used as bleaching agents of human hair, because acidic conditions cause much less damage to the hair and the skin around the hair than alkaline conditions. The enzymatic removal of phenolic compounds from wastewater has been studied using peroxidases (1,5). During the treatment with peroxidase, phenols are oxidized in the presence of hydrogen peroxide to form the corresponding radicals, which react to form insoluble polymers within several minutes (37).…”

Section: Discussionmentioning

confidence: 99%

Purification, Characterization, and Gene Cloning ofCeriporiopsissp. Strain MD-1 Peroxidases That Decolorize Human Hair Melanin

Nagasaki

Kumazawa

Murakami

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

Ceriporiopsis sp. strain MD-1, isolated from forest soil, produced several extracellular enzymes that decolorized human hair melanin. Among them, three enzymes (E1, E2-1, and E2-2) were purified to homogeneity and characterized. The enzymes required hydrogen peroxide in their enzyme reactions and, typical of other fungal peroxidases, oxidized various phenol compounds such as guaiacol, but not 3,4-dimethoxybenzyl alcohol. The spectra of the three enzymes showed an absorption maximum at 406 nm, indicating that they were heme proteins. However, the A 406 /A 280 values of the enzymes were below 0.4, which was lower than those of other peroxidases. E2-1 and E2-2 were similar to each other in their molecular and catalytic properties, and they possibly represent products of posttranslational modifications and/or allelic variants of the same gene, mdcA. The corresponding cDNA was cloned and sequenced; the deduced amino acid sequence showed high identities to the manganese peroxidases from other microorganisms. The specific activities and K m values of E2-1 and E2-2 for synthetic and human hair melanins were much higher than those of Phanerochaete chrysosporium manganese peroxidase and lignin peroxidase.

show abstract

Section: Discussionmentioning

confidence: 99%

Purification, Characterization, and Gene Cloning ofCeriporiopsissp. Strain MD-1 Peroxidases That Decolorize Human Hair Melanin

Nagasaki

Kumazawa

Murakami

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Each tube had a volume of 70 cm 3 and served as a reactor. A storage tank and syringes were connected to the ®rst reactor through a peristaltic pump and a syringe pump, respectively.…”

Section: Plug-flow Operation and Semi-batch Operation Of Plug-flow Symentioning

confidence: 99%

“…The assay used 100 mm 3 of both reagents with 800 mm 3 of sample or sample diluted with distilled water. The absorbance was measured at 510 nm versus reagent blank.…”

Section: Colorimetric Assay For Phenolmentioning

confidence: 99%

Kinetic model-aided reactor design for peroxidase-catalyzed removal of phenol in the presence of polyethylene glycol

Taylor

Biswas

et al. 1999

J. Chem. Technol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

A batch-mixed reactor and a plug-¯ow reactor were shown to have identical reaction kinetics for peroxidase-catalyzed removal of phenol. Semi-batch operation and step-addition operation of the plug-¯ow reactor with respect to hydrogen peroxide produced similar output. In the presence of polyethylene glycol, a continuous stirred tank reactor needed a long time to complete the reaction at the minimum enzyme dose. It was not necessary to stir the reaction mixture continuously during the reaction, hence polymerization and settling of products happened simultaneously. Recycling of the precipitates was not bene®cial either for the removal ef®ciency or for the operation of a settling tank. A model previously developed to simulate the reaction in a batch reactor was used to predict the process in different types of reactor system. A plug-¯ow reactor system is recommended for the reaction in the presence of polyethylene glycol; the system consisting of a small mixing tank followed by a tank in which reaction and settling occur simultaneously.

show abstract

“…A fungal peroxidase from Coprinus macrorhizus was used by Al-Kassim et al (1994) to treat aqueous phenols in batch reactors. Chloroperoxidase from the fungus Caldariomyces fumago has been reported to oxidize several phenolic compounds.…”

Section: (A) Aromatic Pollutantsmentioning

confidence: 99%

Environmental applications of enzymes

Nicell¹

2001

IER

View full text Add to dashboard Cite

Enzymatic processes fall between the two traditional categories of chemical and biological treatment systems since they involve chemical reactions based on the action of biological catalysts. A variety of enzymes from plants and microorganisms have been reported to play important roles in an array of waste treatment applications. Before the full potential of enzymes may be realized, a number of significant issues remain to be addressed. These include: development of low-cost sources of enzymes in quantities that are required at the industrial scale; demonstration of the feasibility of utilizing the enzymes efficiently under the conditions encountered during wastewater treatment; characterization of reaction products and assessment of their impact on downstream processes or on the environment into which they are released; and identification of methods for the disposal of solid residues, among others.

show abstract

Enzymatic removal of selected aromatic contaminants from wastewater by a fungal peroxidase from Coprinus macrorhizus in batch reactors

Cited by 63 publications

References 10 publications

Purification, Characterization, and Gene Cloning ofCeriporiopsissp. Strain MD-1 Peroxidases That Decolorize Human Hair Melanin

Purification, Characterization, and Gene Cloning ofCeriporiopsissp. Strain MD-1 Peroxidases That Decolorize Human Hair Melanin

Kinetic model-aided reactor design for peroxidase-catalyzed removal of phenol in the presence of polyethylene glycol

Environmental applications of enzymes

Contact Info

Product

Resources

About