Enzymatic Saccharification of Acid/Alkali Pre-treated, Mill-run, and Depithed Sugarcane Bagasse

Abstract

aIn South Africa, approximately 3 × 10 6 tons of sugarcane bagasse is produced annually by 14 factories located on the north coast of KwaZuluNatal. It is one of the most readily available lignocellulosic materials for ethanol production through enzymatic saccharification and hydrolysis. Pre-treatment enables disruption of the naturally resistant structure of lignocellulosic biomass to make the cellulose accessible to hydrolysis for conversion to biofuels. In this study, pre-treatment of depithed bagasse and mill-run bagasse was done using acid (3% H2SO4 v/v) followed by alkali (4% NaOH w/v), and the pre-treated solid was subjected to enzymatic hydrolysis. The effects of different conditions for enzymatic saccharification such as enzyme dose, reaction time, and amount of surfactant were studied in detail. The pre-treated substrate (10% w/v) when hydrolysed using 30 FPU/gds/40 FPU/g dry substrate (gds) with 0.4% (v/v) Tween® 80 for 20 h resulted in 608 mg/gds (depithed bagasse) and 604 mg/gds (mill-run bagasse) total reducing sugars.

Underutilized Lignocellulosic Waste as Sources of Feedstock for Biofuel Production in Developing Countries

“…Several microorganisms have been studied as alternative sources of cellulase for hydrolysis of cellulose; some of the reported organisms for this purpose include Candida tropicalis, Trichoderma harzianum, Aspergillus niger, Fusarium oxysporum, and Trichoderma sp. (Mattam et al, 2016;Mezule et al, 2016;Mkhize et al, 2016;Mishra and Jana, 2017). Recently, some authors (Hwangbo et al, 2019) reported a one-step saccharification of pretreated lignocellulosic biomass using immobilized magnetite biocatalyst system.…”

Catalytic condensation of depithed sugarcane bagasse derived levulinic acid into diphenolic acid

“…The DSB was dried in an oven (Scientific, South Africa) at 105 °C for 24 h, milled using a Pulverisette 16 (Fritsch, Germany), and sieved to 40-mesh particle size. The procedure used for compositional analysis of DSB is explained in our previous study (Mkhize et al 2016).…”

“…When the pith is removed from SB, the resulting SB is called depithed sugarcane bagasse (DSB) (Mthembu et al 2020). When original bagasse (mill-run bagasse) was compared with the depithed sugarcane bagasse (Mkhize et al 2016), it was discovered that DSB is easier to handle, and it contains slightly more glucose. Hence, DSB was used in this study.…”

Produção de butanol por fermentação de hidrolisado lignocelulósico com recuperação integrada de produto

“…Portanto, a influência negativa da concentração inicial de glicose no rendimento do produto pode ser atribuída a uma combinação desses fatores complexos relacionados à bioquímica e à fisiologia microbiana. A porcentagem de biomassa solubilizada no pré-tratamento foi de 40% e as composições químicas do bagaço de cana-de-açúcar pré-tratado obtidas no presente estudo apresentaram-se similares aos valores reportados na literatura (Chen, Tu, Sheen, 2022;Mkhize, et al 2016;Rocha, et al 2011).…”