Enzymatic saccharification of lignocellulosic residues using cellulolytic enzyme extract produced by Penicillium roqueforti ATCC 10110 cultivated on residue of yellow mombin fruit

Ferraz, José Lucas de Almeida Antunes; Souza, Lucas Oliveira; Soares, Glêydison Amarante; Coutinho, Janclei Pereira; Oliveira, Julieta Rangel de; Aguiar‐Oliveira, Elizama; Franco, Marcelo

doi:10.1016/j.biortech.2017.06.048

Cited by 49 publications

(7 citation statements)

References 40 publications

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“…Enzymes can interact with ionic species present in the reaction medium. Ions can act as co-factors, either enhancing or inhibiting enzymatic activity as a result of the formation of non-active complexes with the enzyme [49]. Similar results have been reported for xylanase produced by Penicillium roquefortii compared to a commercial xylanase.…”

Section: Discussionsupporting

confidence: 77%

Purification and Characterization of Xylanase Produced by Aspergillus fumigatus Isolated from the Northern Border Region of Saudi Arabia

Ameen

2023

Fermentation

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The purpose of the current work is to produce xylanase from certain agro-industrial wastes in an efficient and effective manner. The culture conditions for three strains of Aspergillus fumigatus are optimized in submerged fermentation (SmF). The most prolific strain (A. fumigatus KSA-2) produces the maximum xylanase at pH 9.0, 30 °C, after 7 days using yeast extract as a nitrogen supply. Aspergillus fumigatus KSA-2 is utilized to produce xylanase at optimum conditions from several agro-industrial wastes. Wheat bran is found to be the most fermentable material, yielding 66.0 U per gram dry substrate (U/gds). The generated xylanase is partly purified using 70% ammonium sulphate, yielding 40 g of dry enzyme powder from 400 g wheat bran. At pH 6.0 and 45 °C, the synthesized xylanase displayed its maximum activity (20.52 ± 1.714 U/mg). In the current study, the effect of ions and inhibitors on xylanase activity is investigated. Both Cu2+ and Mn2+ ions boost the specific activity over the control by 10.2% and 128.0%, respectively. The xylanase enzyme generated has a maximum activity of 4.311 ± 0.36 U/mL/min and the greatest specific activity of 20.53 ± 1.714 U/mg for birchwood xylan, showing a strong affinity for this substrate as opposed to the other xylan and non-xylan substrates.

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Section: Discussionsupporting

confidence: 77%

Purification and Characterization of Xylanase Produced by Aspergillus fumigatus Isolated from the Northern Border Region of Saudi Arabia

Ameen

2023

Fermentation

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“…Second, the fermentation broth was consistently kept in an alkaline condition, which could have led to the swelling of lignocellulosic biomass, and, thus, increased the internal surface area and caused the separation of structural linkages between carbohydrates and lignin [ 42 , 43 ]. Third, the lignin could have absorbed the commercial enzymes and, thus, decreased the availability of active enzymes for hydrolysis [ 44 , 45 ]. The strain G22 secretes abundant amounts of proteins during its growth process, which blocks the surface of lignin, and thus, indirectly increases the availability of usable enzymes for hydrolysis [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Improving enzymatic digestibility of wheat straw pretreated by a cellulase-free xylanase-secreting Pseudomonas boreopolis G22 with simultaneous production of bioflocculants

Guo¹,

Hong²,

Zheng³

et al. 2018

Biotechnol Biofuels

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BackgroundXylan removal by bacterial pretreatments has been confirmed to increase the digestibility of biomass. Here, an effective xylan removal technique has been developed to enhance the digestibility of wheat straw and simultaneously produce bioflocculants by a cellulase-free xylanase-secreting strain, Pseudomonas boreopolis G22.ResultsThe results indicated that P. boreopolis G22 is an alkaliphilic strain which can secrete abundant amounts of xylanase. This xylanase had activity levels of 2.67–1.75 U mL−1 after an incubation period of 5–25 days. The xylanase showed peak activity levels at pH 8.6, and retained more than 85% relative activity in the pH range of 7.2–9.8. After 15 days of cultivation, the hemicellulose contents of the wheat straw were significantly decreased by 32.5%, while its cellulose contents were increased by 27.3%, compared to that of the control. The maximum reducing sugars released from the 15-day-pretreated wheat straw were 1.8-fold higher than that of the untreated wheat straw, under optimal enzymatic hydrolysis conditions. In addition, a maximum bioflocculant yield of 2.08 g L−1 was extracted from the fermentation broth after 15 days of incubation. The aforementioned bioflocculants could be used to efficiently decolorize a dye solution.ConclusionsThe results indicate that the cellulase-free xylanase-secreting P. boreopolis G22 may be a potential strain for wheat straw pretreatments. The strain G22 does not only enhance the enzymatic digestibility of wheat straw, but also simultaneously produces a number of bioflocculants that can be used for various industrial applications.

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“…In addition to the main use of this microorganism in the production of blue cheese, P. roqueforti has also been considered for other biotechnological purposes, such as the production of different metabolites, including the immunosuppressant agent mycophenolic acid [23], lipase extracts on solid state fermentation using cocoa shells as a substrate [24], or cellulolytic enzyme extracts upon cultivation on residue of yellow mombin fruit [25].…”

Section: Brief Overview Of Taxonomic and Biotechnological Aspects Of ...mentioning

confidence: 99%

Secondary Metabolites Produced by the Blue-Cheese Ripening Mold Penicillium roqueforti; Biosynthesis and Regulation Mechanisms

Chávez

Vaca

Estrada

2023

JoF

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Filamentous fungi are an important source of natural products. The mold Penicillium roqueforti, which is well-known for being responsible for the characteristic texture, blue-green spots, and aroma of the so-called blue-veined cheeses (French Bleu, Roquefort, Gorgonzola, Stilton, Cabrales, and Valdeón, among others), is able to synthesize different secondary metabolites, including andrastins and mycophenolic acid, as well as several mycotoxins, such as Roquefortines C and D, PR-toxin and eremofortins, Isofumigaclavines A and B, festuclavine, and Annullatins D and F. This review provides a detailed description of the biosynthetic gene clusters and pathways of the main secondary metabolites produced by P. roqueforti, as well as an overview of the regulatory mechanisms controlling secondary metabolism in this filamentous fungus.

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Enzymatic saccharification of lignocellulosic residues using cellulolytic enzyme extract produced by Penicillium roqueforti ATCC 10110 cultivated on residue of yellow mombin fruit

Cited by 49 publications

References 40 publications

Purification and Characterization of Xylanase Produced by Aspergillus fumigatus Isolated from the Northern Border Region of Saudi Arabia

Purification and Characterization of Xylanase Produced by Aspergillus fumigatus Isolated from the Northern Border Region of Saudi Arabia

Improving enzymatic digestibility of wheat straw pretreated by a cellulase-free xylanase-secreting Pseudomonas boreopolis G22 with simultaneous production of bioflocculants

Secondary Metabolites Produced by the Blue-Cheese Ripening Mold Penicillium roqueforti; Biosynthesis and Regulation Mechanisms

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