Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01

Aggarwal, Neeraj; Goyal, Vipul; Saini, Anita; Yadav, Anita; Gupta, Ranjan

doi:10.1007/s13205-017-0755-0

Cited by 47 publications

(23 citation statements)

References 52 publications

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“…By increasing the pH, the enzyme activity decreased and at pH 7 the enzyme possessed about 40% of its initial activity ( Figure 2). this result is consistent with other researches concerned with the production of cellulases by other Aspergillus species, expressing the optimum activity in acidic condition [17][18][19].…”

Section: Effect Of Ph and Temperature On The Enzyme Activity And Stabsupporting

confidence: 93%

The impact of Egyptian thermophylic cellulase on the dyeability of natural and recovered cellulosic fabrics

Hussien¹,

Allam

Ismail³

et al. 2020

Egypt. J. Chem.

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B IORemeDIAtION using enzymes is an important tool in textile industry; therefore the goal of the present study is to subrogate the pretreatment of natural (cotton) and recovered (viscose) cellulosic fabrics with microbial acidothermophilic cellulase produced by the native egyptian fungus Aspergillus terreus RS2. the produced enzyme was examined at two activity levels (10 and 20U/mL) in order to reduce the effluent load that was produced from scouring and bleaching processes and to upgrade a naturally adequate transaction for water and power economy. the effectiveness of the enzymatic pretreatment under the optimum conditions on raw, scoured and bleached cellulosic fabrics has been proved as the results indicated an increase in the color intensity of the treated fabrics in compare to the untreated one for different classes of reactive dyes based on Anthraquinones and Double azo. the fastness properties of the pretreated dyed fabrics were implemented. the contact angle for the pretreated cellulosic fabrics, tensile strength, Scanning electron microscopy and FtIR analyses were performed.

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Section: Effect Of Ph and Temperature On The Enzyme Activity And Stabsupporting

confidence: 93%

The impact of Egyptian thermophylic cellulase on the dyeability of natural and recovered cellulosic fabrics

Hussien¹,

Allam

Ismail³

et al. 2020

Egypt. J. Chem.

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“…After enzymatic saccharification, the solid residue and supernatant were separated by centrifugation (4°C for 10 min at 5000 rpm) and subsequently the reducing sugar content in the supernatant was analyzed. The extent of cellulase‐mediated saccharification of delignified AV leaf rind was calculated using Equation (1), (Aggarwal, Goyal, Saini, Yadav, & Gupta, 2017)

Saccharification (() %) = \frac{[Reducing sugar obtained \times 0.9]}{Cellulose content in the substrate} \times 100

…”

Section: Methodsmentioning

confidence: 99%

Saccharolysis of laccase delignified Aloe vera leaf rind and fermentation through free and immobilized yeast for ethanol production

Rajeswari

Jacob

2020

J Food Process Engineering

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Aloe vera gel has found prominent application in food, therapeutic and cosmetics manufacturing that eventually increased the generation of leaf rind as processing waste. This biomass was previously delignified by laccase to have better accessibility of holocellulose and an attempt has been made through this study to optimize enzymatic hydrolysis using crude cellulase (5.25 [U/ml]) produced from Aspergillus sp. A maximum saccharification of 63.00 ± 0.45% was achieved under the optimum conditions (solid‐to‐liquid ratio 1:17 [wt/vol], 53°C and 8.5 hr) with sugar yield of 309 ± 2.1 mg/g. Fermentation of saccharified liquid to ethanol using Saccharomyces cerevisiae (yeast) was performed using three different modes of inoculum application such as free cell suspension; Ca‐alginate immobilized yeast suspension and immobilized yeast loaded in a packed bed reactor (PBR) under uniform process conditions. Immobilized yeast in PBR has resulted in maximum ethanol yield of about 16.50 ± 1.02 g/L with a fermentation efficiency of 76.14%. Practical application In a global scenario of diminishing resources, there is dire need to establish the cradle‐to‐grave approach for each of industrial operations. Especially, with food processing industries and its waste management this became truly inevitable, as these wastes are rich in nutrient and perishable in nature. On the other hand, it could also serve as a potent source for value added products. Thus, the utilization of Aloe vera (AV) leaf rind to produce bioethanol could underpin the AV gel processing industries toward circular bioeconomy as an option of waste to wealth. The methodology followed to develop this technology is a complete green approach where there is no requirement of chemicals and harsh reagents that remain recalcitrant postprocessing. Adoption of this technology could add value to the sustainable goals and reduce the carbon footprint of the food processing industrial operations.

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“…Thus the highest saccharification yield of 69.91 %, corresponding to reducing sugar concentration of 10.58 g/L or 529 mg/g substrate was achieved with alkaline pretreated straw. The reducing sugar concentration of 600 mg/g was achieved with cellulase from Trichoderma harzianium SNRS3 [11] but only 237.8 mg/g reducing sugar was released by crude cellulase from Aspergilus niger BK01 [12]. The results suggested that cellulase from Cellulosimicrobium sp.…”

Section: Effect Of Ph On the Enzymatic Saccharification Of Pretreated Biomassmentioning

confidence: 96%

Enzymatic Sacharification of Alkaline Pretreated Rice Straw by Cellulase From Cellulosimicrobium Sp. Mp1

Xuan¹,

Ha²,

Tien³

2021

JST

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The effects of different physical and technological parameter such as time, substrate to liquid ratio, enzyme concentration, temperature, and pH on enzymatic saccharification of alkaline pretreated straw cellulose were studied. For alkaline pretreatment, the straw was incubated with 10 % NaOH at ratio 1:20 (w/v) at 90 °C for 1 hour. After the alkaline pretreatment the cellulose content increased from 50.2 % (w/w) to 67.3 % (w/w). Enzyme used for saccharification of treated and untreated straw was produced from Cellulosimicrobium sp MP1 which was isolated from termite gut. Results from research showed that the highest percentage of saccharification of alkaline pretreated straw was 69.91 %, corresponding to 10.58 mg/mL of reducing sugar. The hydrolysis conditions for reaching this highest saccharification yield were: temperature of 55 ºC, substrate to liquid ratio of 2 g/100 mL, enzyme concentration of 37.5 U/g, pH of 5.5 and hydrolysis time of 48 hours.

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Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01

Cited by 47 publications

References 52 publications

The impact of Egyptian thermophylic cellulase on the dyeability of natural and recovered cellulosic fabrics

The impact of Egyptian thermophylic cellulase on the dyeability of natural and recovered cellulosic fabrics

Saccharolysis of laccase delignified Aloe vera leaf rind and fermentation through free and immobilized yeast for ethanol production

Enzymatic Sacharification of Alkaline Pretreated Rice Straw by Cellulase From Cellulosimicrobium Sp. Mp1

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