Enzymatic self-wiring in nanopores and its application in direct electron transfer biofuel cells

Trifonov, Alexander; Stemmer, Andreas; Tel‐Vered, Ran

doi:10.1039/c8na00177d

Cited by 17 publications

(9 citation statements)

References 77 publications

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“…They also lead to favorable orientation of laccase molecules at the cathode due to hydrophobic interaction of naphthoquinone with the T 1 pocket. The power density of the constructed fuel cell was 0.81 mW cm −2 and the OCV was 0.82 V. Highly favorable properties, both in terms of power and OCV, compared to those of other biofuel cells [16,23,44], and in particular compared to the sucrose-based system described by Herkendell et al [42] which is the result of the innovative procedure used for the electrode preparation ( Table 2). The CPPy-AuNP-NQ modification provides not only good catalytic but also capacitive properties of both anode and cathode.…”

Section: Discussionmentioning

confidence: 93%

“…Various nanostructures are proposed for the construction of the electrode to obtain the appropriate orientation of the enzyme relative to the electrode surface and to decrease the distance of electron transport. In the large group of possible nanostructures, platinum and gold nanoparticles, are often employed due to their attractive structural, mechanical and electronic properties [13][14][15][16]. Efficient direct electron transfer (DET) has been demonstrated using porous gold electrodes [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Multi-Substrate Biofuel Cell Utilizing Glucose, Fructose and Sucrose as the Anode Fuels

et al. 2020

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A significant problem still exists with the low power output and durability of the bioelectrochemical fuel cells. We constructed a fuel cell with an enzymatic cascade at the anode for efficient energy conversion. The construction involved fabrication of the flow-through cell by three-dimensional printing. Gold nanoparticles with covalently bound naphthoquinone moieties deposited on cellulose/polypyrrole (CPPy) paper allowed us to significantly improve the catalysis rate, both at the anode and cathode of the fuel cell. The enzymatic cascade on the anode consisted of invertase, mutarotase, Flavine Adenine Dinucleotide (FAD)-dependent glucose dehydrogenase and fructose dehydrogenase. The multi-substrate anode utilized glucose, fructose, sucrose, or a combination of them, as the anode fuel and molecular oxygen were the oxidant at the laccase-based cathode. Laccase was adsorbed on the same type of naphthoquinone modified gold nanoparticles. Interestingly, the naphthoquinone modified gold nanoparticles acted as the enzyme orienting units and not as mediators since the catalyzed oxygen reduction occurred at the potential where direct electron transfer takes place. Thanks to the good catalytic and capacitive properties of the modified electrodes, the power density of the sucrose/oxygen enzymatic fuel cells (EFC) reached 0.81 mW cm−2, which is beneficial for a cell composed of a single cathode and anode.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Multi-Substrate Biofuel Cell Utilizing Glucose, Fructose and Sucrose as the Anode Fuels

et al. 2020

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“…Similar to other carbonaceous nanostructures, [23][24][25][26] DET between the T1 blue copper center of BOD and the mpCNP matrix occurs, 27,28 enabling electrocatalysis in the absence of any additional mediating relays. At this stage, CAT-loaded ccMNPs were introduced into the electrolyte solution concurrently with a magnet placed behind the BODfunctionalized mpCNPs/GC assembly.…”

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confidence: 89%

Magnetically induced enzymatic cascades – advancing towards multi-fuel direct/mediated bioelectrocatalysis

2019

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“…Various types of amperometric glucose biosensors have been reported [ 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. SMBG sensors involve FAD-dependent glucose oxidase (GOD) [ 60 , 61 ], bacterial and fungal FAD-dependent glucose dehydrogenase (FAD-GDH) [ 62 , 63 ], PQQ-dependent soluble GDH (PQQ–sGDH) [ 64 , 65 , 66 ] and NAD-dependent GDH (NAD-GDH) [ 67 ]. Characteristics of the enzymes are summarized in a review [ 68 ].…”

Section: Fundamentals Of Bioelectrocatalytic Sensorsmentioning

confidence: 99%

Development Perspective of Bioelectrocatalysis-Based Biosensors

Adachi

Kitazumi

Shirai

et al. 2020

Sensors

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Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.

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Enzymatic self-wiring in nanopores and its application in direct electron transfer biofuel cells

Abstract: Direct electron transfer bioelectrocatalysis through synthesized metal nanoclusters in confined pores.

Cited by 17 publications

References 77 publications

Multi-Substrate Biofuel Cell Utilizing Glucose, Fructose and Sucrose as the Anode Fuels

Multi-Substrate Biofuel Cell Utilizing Glucose, Fructose and Sucrose as the Anode Fuels

Magnetically induced enzymatic cascades – advancing towards multi-fuel direct/mediated bioelectrocatalysis

Development Perspective of Bioelectrocatalysis-Based Biosensors

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