Enzymatic Staining for Detection of Phenol-Oxidizing Isozymes Involved in Lignin- Degradation by Lentinula edodes on Native-PAGE

Tanesaka, Eiji; Saeki, Naomi; Kochi, Akinori; Yoshida, Motonobu

doi:10.5772/37815

Cited by 2 publications

(3 citation statements)

References 48 publications

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“…However, when cultured in liquid medium supplemented with the sawdust extract, L. edodes produced either MnP or Lcc, but not both. These observations suggest that the expression of MnP and/or Lcc in L. edodes is controlled by a negative feedback interaction, i.e., where MnP production inhibits Lcc production and MnP-inhibition enhances Lcc production Tanesaka et al, 2012 . This study therefore examined the expression of phenol-oxidizing enzymes in L. edodes under liquid culture conditions in response to the addition of varying combinations of the following putative inducers of phenol-oxidizing enzymes: different concentrations of sawdust extract, 10 mM aromatic compounds, and 1 or 2 mM Cu 2+ . Implications of the results in the regulation of phenol-oxidizing enzyme expression employed by this fungus are also discussed.…”

Section: Enzyme Assaymentioning

confidence: 88%

“…The finding indicated that sawdust extract induced the secretion of the same isozyme, LeMnP2, which is a major MnP isozyme that is secreted into sawdust media Sakamoto et al, 2009 . The results of the Northern blotting analysis with a probe lemnp2 are shown in Tanesaka et al, 2012. observed in cultures grown in MYPG-S 500 , and not in MYPG-S 100 . These observations suggested that MnP is only enhanced by specific, concentration-dependent, functional compounds in the high-concentration sawdust extract MYPG-S 500 .…”

Section: Manganese Peroxidase Gene Transcriptionmentioning

confidence: 93%

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Phenol-Oxidizing Enzyme Expression in Lentinula edodes by the Addition of Sawdust Extract, Aromatic Compounds, or Copper in Liquid Culture Media

2013

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This study examined how the addition of a sawdust extract from Castanopsis cuspidata, several aromatic compounds, and copper affected the expression of a phenol-oxidizing enzyme in the white-rot basidiomycete, Lentinula edodes. Compared to liquid media that had not been supplemented with sawdust extract (MYPG), MYPG containing low (MYPG-S100) or high (MYPG-S500) concentrations of sawdust extract had a marked effect on the promotion of mycelial growth. No manganese peroxidase (MnP) production was observed in either MYPG or MYPG-S100 media until 35 days after inoculation. However, MnP production was enhanced by culture in MYPG-S500, with a marked increase observed suddenly at 14 days after inoculation. Northern blot analysis revealed that the transcription of the lemnp2 gene coding extracellular MnP was initially observed at detectable levels at day 10 after the initial inoculation of MYPG-S500, increasing gradually thereafter until days 22-25. However, laccase (Lcc) production was not observed in any of the media until 35 days after inoculation. Addition of 10 mM aromatic compounds - 1,2-benzenediol, 2-methoxyphenol, hydroquinone, and 4-anisidine--into the MYPG-S500 medium completely inhibited MnP production and did not enhance any Lcc production. While the addition of 1 or 2 mM Cu2+ (CuSO4 x 5H2O) to MYPG-S500 medium completely inhibited MnP production, this Cu2+ addition caused a marked increase in Lcc production at 17 and 6 days after the addition, respectively.

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Section: Enzyme Assaymentioning

confidence: 88%

Section: Manganese Peroxidase Gene Transcriptionmentioning

confidence: 93%

Phenol-Oxidizing Enzyme Expression in Lentinula edodes by the Addition of Sawdust Extract, Aromatic Compounds, or Copper in Liquid Culture Media

2013

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“…This commercial mushroom is a whiterot fungus, which belongs to the family Tricholomataceae (Ma et al, 2010;Saritha et al, 2012) and a diverse group of Basidiomycetes that attack the biologically stable polymer of plants, lignocellulose (Dorado et al, 2001;Jurado et al, 2011;Pandya and Albert, 2014). The ability of white-rot fungi to degrade plants essentially depends on the type and ecology of the fungus involved (Bisen et al, 2010;Tanesaka et al, 2004). Generally, P. ostreatus grows in conditions that would allow for the enzymebased conversion reactions to take place, and such conditions include carbon source, substrate, temperature, light, pH, and humidity (Jurado et al, 2011;Kerem et al, 1992;Patel et al, 2009;Tien and Kirk, 1983).…”

Section: Introductionmentioning

confidence: 99%

Exploring for the possibility of utilizing Pleurotus ostreatus to manage Eichhornia crassipes in Zimbabwe

Sibanda¹,

Ruzvidzo²,

Zvidzai³

et al. 2019

J. Yeast Fungal Res.

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The versatile nature of the commercial mushroom, Pleurotus ostreatus (Jacq.: Fr.) Kumm., provides basis for continued exploration of its biochemical processes during solid state fermentation on various lignocellulosic biomass as potential substrates. As a fungal organism, P. ostreatus feeds on lignocellulose by secreting various extracellular enzymes responsible for breaking down this organic polymer into smaller and simple compounds that the hyphae can absorb to develop into mycelia. In this study, P. ostreatus was assessed for its ability to grow on Eichhornia crassipes (Mart.) Solms-Laubach., or water hyacinth biomass and possibly, utilizing it as a substrate. E. crassipes is an aquatic herbaceous plant, often branded as the world's worst aquatic weed due to its invasive aggression, negative impact onto the aquatic ecosystems, and the cost usually associated with its control. When cultured onto the biomass of this nuisance weed followed by assessment by the agar plate-based clearing assay method and spectrophotometry, P. ostreatus demonstrated its practical ability to secrete cellulases, xylanases, pectinases, lignin peroxidases and laccases, thus showing its physiological capabilities to optionally utilize E. crassipes as a substrate. If properly optimized, this approach can be remarkably used as a sustainable and cost-effective strategy to manage E. crassipes in Zimbabwe.

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Enzymatic Staining for Detection of Phenol-Oxidizing Isozymes Involved in Lignin- Degradation by Lentinula edodes on Native-PAGE

Cited by 2 publications

References 48 publications

Phenol-Oxidizing Enzyme Expression in Lentinula edodes by the Addition of Sawdust Extract, Aromatic Compounds, or Copper in Liquid Culture Media

Phenol-Oxidizing Enzyme Expression in Lentinula edodes by the Addition of Sawdust Extract, Aromatic Compounds, or Copper in Liquid Culture Media

Exploring for the possibility of utilizing Pleurotus ostreatus to manage Eichhornia crassipes in Zimbabwe

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