1980
DOI: 10.1016/0009-8981(80)90135-7
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Enzymatic studies of urinary isomeric chondroitin sulfates from patients with mucopolysaccharidoses. The application of high performance liquid chromatography

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Cited by 21 publications
(4 citation statements)
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“…The semi-quantitative test for glycosaminoglycan (GAGs) hyperexcretion was performed using standard methods (Pennock 1976). One-and two-dimensional electrophoresis of GAGs (isolated from urine, amniotic fluid, brain and liver autopsy samples according to Lee et al (1980) with minor modifications) were run on celli~gel strips (Serva, Germany) (Whiteman and Henderson, 1977;Hronovsky and Anastassiades, 1979). GAG standards chondroitin sulphates (ChS) A and C, dermatan sulphate (DS) were purchased from Seikagaku Kogio, Japan.…”
Section: Metaboiitesmentioning
confidence: 99%
“…The semi-quantitative test for glycosaminoglycan (GAGs) hyperexcretion was performed using standard methods (Pennock 1976). One-and two-dimensional electrophoresis of GAGs (isolated from urine, amniotic fluid, brain and liver autopsy samples according to Lee et al (1980) with minor modifications) were run on celli~gel strips (Serva, Germany) (Whiteman and Henderson, 1977;Hronovsky and Anastassiades, 1979). GAG standards chondroitin sulphates (ChS) A and C, dermatan sulphate (DS) were purchased from Seikagaku Kogio, Japan.…”
Section: Metaboiitesmentioning
confidence: 99%
“…GAG was isolated from the urine and the isomeric chondroitin sulphates were studied by the method of Lee et al (1980), with a high-pressure liquid chromatography (HPLC) apparatus (Hitachi 655, Hitachi Ltd., Tokyo) equipped with a UV detector at 254nm (Hitachi 638-41) and a data processing system (Hitachi 655-60). A Partisil-10 PAC column (10 ~m, 25 cmx 4.6 mm i.d.…”
Section: Urinary Glycosaminoglycans (Gag)mentioning
confidence: 99%
“…RP-IP chromatography is also used to separate oligosaccharides using di- and tributyl amines . All eight disaccharides can be separated using a tributyl quaternary amine, and for larger oligosaccharides, a triethyl amine is generally used as the ion pairing agent . This method has been employed in the analysis of sugars from model organisms, such as Caenorhabditis elegans and Drosophila .…”
mentioning
confidence: 99%