Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules

Eberl, Anita; Heumann, Sonja; Brückner, Tina; Araújo, Rita; Cavaco‐Paulo, Artur; Kaufmann, Franz; Kroutil, Wolfgang; Guebitz, Georg M.

doi:10.1016/j.jbiotec.2009.07.008

Cited by 212 publications

(190 citation statements)

References 24 publications

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“…PET and 3PET soluble released products were analyzed as previously described by Eberl et al [32]. After enzymatic treatment proteins were precipitated using 1:1 (v/v) methanol abs.…”

Section: Analysis Of Pet 3pet and Pla Hydrolysis Released Productsmentioning

confidence: 99%

“…Hydrolysis of the model substrate bis(benzoyloxyethyl)terephthalate (3PET) has been proven first to be valuable for the screening of enzymes capable of PET hydrolysis [40] and to provide important mechanistic information [27,32,34,42] about the type of hydrolysis mechanism. In the case of the new esterase Thh_Est, the hydrolysis product found in highest concentration was BA, followed by HEB, both in a comparable concentration higher than 50 mmol per mol of enzyme.…”

Section: Hydrolysis Of the Pet Model Substrate Bis(benzoyloxyethyl)tementioning

confidence: 99%

“…Cutinases [30][31][32], lipases [29,32], esterases [26,30,33] including paranitrobenzyl esterase [34] have been assessed for this purpose.…”

Section: Introductionmentioning

confidence: 99%

“…Considerably fewer lipases have been described to hydrolyze PET, including enzymes from Candida antarctica [25], Thermomyces lanuginosus [32], Burkholderia spp. [38] and Triticum aestivum [39].…”

Section: Introductionmentioning

confidence: 99%

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A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA)

et al. 2012

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Abstract:A new esterase from Thermobifida halotolerans (Thh_Est) was cloned and expressed in E. coli and investigated for surface hydrolysis of polylactic acid (PLA) and polyethylene terephthalate (PET). Thh_Est is a member of the serine hydrolases superfamily containing the -GxSxG-motif with 85-87% homology to an esterase from T. alba, to an acetylxylan esterase from T. fusca and to various Thermobifida cutinases. Thh_Est hydrolyzed the PET model substrate bis(benzoyloxyethyl)terephthalate and PET releasing terephthalic acid and mono-(2-hydroxyethyl) terephthalate in comparable amounts (19.8 and 21.5 mmol/mol of enzyme) while no higher oligomers like bis-(2-hydroxyethyl) terephthalate were detected. Similarly, PLA was hydrolyzed as indicated by the release of lactic acid. Enzymatic surface hydrolysis of PET and PLA led to a strong hydrophilicity increase, as quantified with a WCA decrease from 90.8° and 75.5° to 50.4° and to a complete spread of the water drop on the surface, respectively. OPEN ACCESSPolymers 2012, 4 618

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“…PET and 3PET soluble released products were analyzed as previously described by Eberl et al [32]. After enzymatic treatment proteins were precipitated using 1:1 (v/v) methanol abs.…”

Section: Analysis Of Pet 3pet and Pla Hydrolysis Released Productsmentioning

confidence: 99%

Section: Hydrolysis Of the Pet Model Substrate Bis(benzoyloxyethyl)tementioning

confidence: 99%

“…Cutinases [30][31][32], lipases [29,32], esterases [26,30,33] including paranitrobenzyl esterase [34] have been assessed for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA)

et al. 2012

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“…Ebert et al [43] used lipase from Thermonospora lanuginosus and cutinase from Thermomonospora fusca and Fusarium solani to hydrolyze polyethylene terephthalate. Action of lipase in the presence of Triton-X-100 resulted in the formation of novel polar groups that enhanced the dyeability to as much as 130% for cutinase and 300% in the case of lipase.…”

Section: Polyester Fabric Modification By Some Lipasesmentioning

confidence: 99%

Role of Biotechnology in the Treatment of Polyester Fabric

Wavhal

Balasubramanya

2011

Indian J Microbiol

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Poly (ethylene terephthalate) fibre [PET] is the commonly used fibre for majority of end-use applications, however, the desire for improved textile properties such as wettability or hydrophilicity are increasing. Biotechnology can be defined as the application of scientific and engineering to the processing of materials by biological agents to provide goods and services. The environmental issues associated with the textile processing are not new. Currently and in the years to come, besides lower cost of operation, improved durability, wear comfort and development of new attributes for textiles, the new criteria for judging the new processes is ecology. This paves the way for biotechnology. This article throws light on the applications of enzymes for the treatment of polyester fabrics.

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Enzymes in Industrial Biotechnology

Lobedanz

Damhus

Borchert

et al. 2016

Kirk-Othmer Encyclopedia of Chemical Technology

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Enzyme technology has been used in industrial processes since ancient Greece. The present industrial applications of enzymes are, however, the result of a rapid development seen primarily over the past five decades, thanks to, first of all, the evolution of modern biotechnology. The result is a highly diversified market that is still growing in terms of both size and complexity. The introduction of protein engineering has revolutionized the development of industrial enzymes further and made it possible to tailor‐make enzymes to display new activities and adapt to new process conditions enabling a further expansion of their use. Production of enzymes involves recombinant expression in selected host microorganisms, fermentation, recovery, and formulation. The detergent industry is the largest user of enzymes, which are utilized to remove soiling, increase softness, and protect garments. The starch industry uses a variety of enzymes in the production of value‐added products from starches. In the bioethanol industry, enzymes are used to convert starch and biomass into fermentable sugars. Enzymes also find wide use in the textile, leather, pulp and paper, and animal feed industries. Another important area is the food industry in which enzymes are used in the dairy, bakery, brewing, and protein‐modification industries.

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Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules

Cited by 212 publications

References 24 publications

A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA)

A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA)

Role of Biotechnology in the Treatment of Polyester Fabric

Enzymes in Industrial Biotechnology

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