Enzymatic synthesis of ATP from RNA and adenine

Abstract

The title compound was prepared by a three-stage enzymatic procedure consisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact myceUum of Spicaria violacea, (ii) transribosylation of exogenous adenine employing whole cells of Escherichia coil as a biocatalyst, and (iii) conversion of formed adenosine into ATP by the enzymes of alcohol fermentation and the kinases extracted from baker's yeast.

One-step isolation of adenosine triphosphate from crude fermentation broth of Saccharomyces cerevisiae by anion-exchange chromatography using supermacroporous cryogel

“…Due to the high costs of starting materials or the difficult additional separations, these methods were seldom applied in industrial scale. In the recent decades biosynthesis means using enzymes (polyphosphate kinase, acetate kinase, adenylate kinase, pyruvate kinase and polyphosphate-AMP phosphotransferase) coupled with special substrates [1][2][3][4] or using microbial cells (Saccharomyces cerevisiae, Candida boidinii, Brevibacterium ammoniagenes, Escherichia coli) with the required enzymatic system [1,[5][6][7][8][9] has been applied to prepare ATP. Among them phosphorylation of AMP by yeast cells to produce ATP is an economical and easy way in industrial processes and employed in many production plants.…”