Enzymatic synthesis of chlorogenic acid glucoside using dextransucrase and its physical and functional properties

Abstract

Chlorogenic acid, a major polyphenol in edible plants, possesses strong antioxidant activity, anti-lipid peroxidation and anticancer effects. It used for industrial applications; however, this is limited by its instability to heat or light. In this study, we for the first time synthesized chlorogenic acid glucoside (CHG) via transglycosylation using dextransucrase from Leuconostoc mesenteroides and sucrose. CHG was purified and its structure determined by nuclear magnetic resonance and matrix-associated laser desorption ionization-time-of-flight mass spectroscopy. The production yield of CHG was 44.0% or 141mM, as determined by response surface methodology. CHG possessed a 65% increased water solubility and 2-fold browning resistance while it displayed stronger inhibition of lipid peroxidation and of colon cancer cell growth by MTT assay, compared to chlorogenic acid. Therefore, this study may expand the industrial applications of chlorogenic acid as water-soluble or browning resistant compound (CHG) through enzymatic glycosylation.

Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry

“…Lactiplantibacillus plantarum fermentation significantly enhanced the ability to scavenge free radical's DPPH when the fermenting conditions were optimized by the method of responsive surface design in fermenting sheep bone Produce antioxidant substances (active phenol metabolites, chlorogenic acid glucoside, sulforaphane) have a variety of beneficial effects on the human body Lactiplantibacillus plantarum (Ge et al, 2019;Mu et al, 2019;Ryu et al, 2019), Lacticaseibacillus rhamnosus, Lactobacillus acidophilus (Kêska and Stadnik, 2018), Leuconostoc mesenteroides (Nam et al, 2017) Metabolize phenolic acid by decarboxylase and reductase…”

Effects of different drying methods on drying kinetics and physicochemical properties of Chrysanthemum morifolium Ramat

“…Medicinal chrysanthemum contains many flavonoids and polyphenols, with chlorogenic acid being one of the main polyphenols, and it has strong antioxidant activity, inhibits lipid peroxidation, and has anticancer effects [5] . In modern times, chrysanthemums are also used as a functional food.…”

The impact of the co‐exposure of melanoma cells to chlorogenic acid and a moderate‐strength static magnetic field

“…Although this effect was already observed at the in vitro level, in vivo studies only confirmed it (Tajik et al, 2017). This is due to the fact that CGA is an electrophilic trapping factor and its ability to inhibit lipid peroxidation, which is triggered by free radicals (Nam et al, 2017). It has also been F I G U R E 5 Effects of chlorogenic acid and a static magnetic field on the antioxidative defense parameters -SOD (5A), GPx (5B) and CAT (5C) activity in the melanoma cells.…”

“…Although this effect was already observed at the in vitro level, in vivo studies only confirmed it (Tajik et al., 2017). This is due to the fact that CGA is an electrophilic trapping factor and its ability to inhibit lipid peroxidation, which is triggered by free radicals (Nam et al., 2017). It has also been proven that that CGA is active against the mutagenesis and carcinogenesis that is induced by various chemical carcinogens, for example, polycyclic aromatic hydrocarbons (Szaefer et al., 2004).…”