1995
DOI: 10.1271/bbb.59.179
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Enzymatic Synthesis ofα-Linked Galactooligosaccharides Using the Reverse Reaction of a Cell-boundα-Galactosidase fromCandida guilliermondiiH-404

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Cited by 18 publications
(6 citation statements)
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“…Amongst these, convincing scientific evidence for their suitability for use as prebiotics exist for inulin/oligofructose and for galactooligosaccharides (GOS) (Gibson et al, 2004). There is a multiplicity of beneficial health effects attributed to the ingestion of GOS (Sako, Matsumoto, & Tanaka, 1999;Moro et al 2002;Playne, Bennett, & 2003;van den Broek, Ton, Verdoes, van Laere, Voragen, & Beldman, 1999) or by reverse reaction of a-galactosidases on lactose hydrolyzates (Hashimoto, Katayama, Goto, Okinaga, & Kitahata, 1995). The main products reported, were a(1-6) linked tri-and tetra-saccharides, in the case of donor substrates, and similarly linked disaccharides on the reverse reaction on lactose hydrolyzates.…”
Section: Introductionmentioning
confidence: 99%
“…Amongst these, convincing scientific evidence for their suitability for use as prebiotics exist for inulin/oligofructose and for galactooligosaccharides (GOS) (Gibson et al, 2004). There is a multiplicity of beneficial health effects attributed to the ingestion of GOS (Sako, Matsumoto, & Tanaka, 1999;Moro et al 2002;Playne, Bennett, & 2003;van den Broek, Ton, Verdoes, van Laere, Voragen, & Beldman, 1999) or by reverse reaction of a-galactosidases on lactose hydrolyzates (Hashimoto, Katayama, Goto, Okinaga, & Kitahata, 1995). The main products reported, were a(1-6) linked tri-and tetra-saccharides, in the case of donor substrates, and similarly linked disaccharides on the reverse reaction on lactose hydrolyzates.…”
Section: Introductionmentioning
confidence: 99%
“…8,9) Despite the hydrolytic activity, a-galactosidase is powerful for oligosaccharide synthesis by transglycosylation, and also by reverse hydrolysis. [10][11][12][13] Therefore, the mechanism for producing polysaccharides of G. lucidum might be related to both the hydrolytic activity and galactosyl transfer activity of a-galactosidase. We isolated and characterized G. lucidum a-galactosidase and found that this enzyme was relatively thermally stable and had the transfer activity for oligosaccharide synthesis.…”
mentioning
confidence: 99%
“…Out of these pathways, only the routes using inverting or retaining trehalose phosphorylases (TP) [ 33 , 42 ] ( Figure 3 e,f) and TreT-type trehalose synthases ( Figure 3 d) [ 34 , 43 ] can be easily adapted towards biocatalytic trehalose analogue synthesis. A third possible enzymatic pathway for analogues production, not applicable for trehalose synthesis, makes use of galactosidases [ 44 , 45 ]. Each of these three routes comes with its own advantages and disadvantages, inherent to the respective enzyme class employed, on which will be further elaborated in this review.…”
Section: Biocatalytic Synthesis Routes For Trehalose Analoguesmentioning
confidence: 99%
“…Galactosidases were the first enzymes ever to be exploited for the synthesis of trehalose di- or trisaccharide analogues. In 1995, a thermostable α-galactosidase (EC 3.2.1.22) from Candida guilliermondii was reported to generate galactotrehalose through reverse hydrolysis when high concentrations of galactose were used as substrate [ 44 ]. Unfortunately, the enzyme was not very selective and preferably formed a 1,6-linkage, with 1,3- and 1,2-isomers being formed as well.…”
Section: Biocatalytic Synthesis Routes For Trehalose Analoguesmentioning
confidence: 99%