Enzymatic Synthesis of Juvenile Hormone in Manduca sexta

Reibstein, David; Law, John H.; Bowlus, Stephen B.; Katzenellenbogen, John A.

doi:10.1007/978-1-4684-7947-8_10

Cited by 21 publications

(18 citation statements)

References 8 publications

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“…The esterification of FA is assumed to precede epoxidation in most insect species, including Orthoptera, Dictyoptera, Coleoptera, and Diptera (Belles et al, 2005). In contrast, the presence of an enzyme that specifically epoxidizes FA to JH acid in the tobacco hornworm, Manduca sexta, implies that the epoxidation of FA precedes esterification in Lepidoptera (Reibstein et al, 1976;Schooley and Baker, 1985). Recent studies revealed that recombinant CYP15A1 of D. punctata (Dictyoptera) catalyzed the epoxidation of MF to JH, but not FA to JH acid (Helvig et al, 2004), while recombinant CYP15C1 of B. mori (Lepidoptera) preferably epoxidized FA over MF (Daimon et al, 2012).…”

Section: Introductionmentioning

confidence: 93%

Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Minakuchi

Ishii

Washidu

et al. 2015

Journal of Insect Physiology

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Section: Introductionmentioning

confidence: 93%

Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Minakuchi

Ishii

Washidu

et al. 2015

Journal of Insect Physiology

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“…Although glands of 6-day animals produced far less JH-I11 acid, 7-day CA synthesized JH-I, JH-11, and JH-111 acids in more comparable amounts. The combined total amount of the acids produced by prepupal CA was at least [10][11][12][13][14][15][16][17][18][19][20] times greater than the total amount of JH and JH acids produced by O-day fifth instar CA. The rather large quantities of JH acids secreted by 6-7-day CA may account for the low morphogenetic activity observed in the fourth instar assay (Table 1).…”

Section: In Vitro Synthesis Of Jh Acids and Jhmentioning

confidence: 99%

Change in corpus allatum function during metamorphosis of the tobacco hornworm Manduca sexta: Regulation at the terminal step in juvenile hormone biosynthesis

Bhaskaran

Sparagana

Barrera

et al. 1986

Arch Insect Biochem Physiol

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Changes in activity of the corpora allata (CA) during larval-pupal-adult development of the tobacco hornworm Manduca sexta were studied by transplantation assays, measurements of in vitro juvenile hormone (JH) and JH acid synthesis, and determination of JH acid methyltransferase OHAMT) and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activities.The data from these assays demonstrate that the CA cease to secrete JH by day 4 of the last larval instar (wandering stage). With regard to JH synthesis, they remain inactive throughout the prepupal, pupal, and most of the pharate adult periods. CA of females, but not of males, resume JH synthesis shortly before eclosion. The biochemical basis of the inactivation process is the loss of JHAMT activity. However, prepupal CA produce JH acids, as shown by enzyme and in vitro assays. Pupal and pharate adult CA do not synthesize JH acids although levels of HMG-CoA reductase activity seem to remain relatively high. Radiolabeled JH was recovered from hemolymph of allatectomized prepupae that had been injected with radiolabeled JH acid. These results provide further evidence that certain peripheral tissues (eg, imaginal discs) convert JH acid secreted by the prepupal CA to JH and, thus, that JH acid is a prohormone in the prepupal period. The CA change from hormone secretion to prohormone secretion during larval-prepupal transformation, a unique functional alteration in an endocrine gland.

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“…5) and no MF was found in these samples. These results suggest strongly that the methyl transferase involved in the esterification step in JH III biosynthesis has no specificity for either farnesoic acid or JH III acid, although it apparently does have specificity for the sesquiterpene acid analogs of the JH homologs (Reibstein et al, 1976).…”

Section: Resultsmentioning

confidence: 96%

“…In an earlier work (Teal et al, 2001), we identi- documented (Reibstein et al, 1976 (Reibstein et al, 1976). To determine if MF was converted to JH III by isolated, but otherwise intact, RC complexes, we incubated tissue from 1-day-old females in the presence of Manse-AST plus MF.…”

Section: Resultsmentioning

confidence: 99%

Identification of methyl farnesoate from in vitro culture of the retrocerebral complex of adult females of the moth, Heliothis virescens (Lepidoptera: Noctuidae) and its conversion to juvenile hormone III

Teal

Proveaux

2006

Arch Insect Biochem Physiol

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Gas chromatographic-mass spectral analysis of extracts obtained from in vitro culture of isolated retrocerebral complexes obtained from adult females of the moth Heliothis virescens resulted in identification of methyl farnesoate as well as juvenile hormone III (JH III) but not JH III acid. Inhibition of JH biosynthesis by incubation of tissue in synthetic Manduca sexta allatostatin (Manse-AST, pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-COOH) reduced production of these chemicals to negligible levels. However, incubation of tissue in the presence of Manse-AST plus farnesol resulted in production of significant amounts of both methyl farnesoate and JH III. Tissue incubated in the presence of Manse-AST plus methyl farnesoate produced only JH III. The results indicated that methyl farnesoate is naturally produced by the corpora allata of adult females of Heliothis virescens. However, tissue incubated in the presence of Manse-AST plus JH III acid also produced JH III in amounts equivalent to that produced by tissue incubated with methyl farnesoate. Thus, both methyl farnesoate and JH III acid could serve as a precursor for biosynthesis of JH III.

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Enzymatic Synthesis of Juvenile Hormone in Manduca sexta

Cited by 21 publications

References 8 publications

Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Change in corpus allatum function during metamorphosis of the tobacco hornworm Manduca sexta: Regulation at the terminal step in juvenile hormone biosynthesis

Identification of methyl farnesoate from in vitro culture of the retrocerebral complex of adult females of the moth, Heliothis virescens (Lepidoptera: Noctuidae) and its conversion to juvenile hormone III

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