Enzymatic synthesis of new antimicrobial peptides for food purposes

Adaro, Mauricio Omar; Ibáñez, Ángel Gabriel Salinas; Origone, Anabella Lucía; Vallés, Diego; Guzmán, Fanny; Vega, Alba Edith; Barberis, S

doi:10.3389/fmicb.2023.1153135

Cited by 2 publications

(1 citation statement)

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“…To date, traditional antibiotics and antibacterial physical treatments have been widely used to inhibit microbial growth and increase the shelf-life of food ( Rai et al, 2016 ; Adaro et al, 2023 ). However, the prolonged use of antibiotics have been associated with the emergence of multi-resistant microorganisms, representing a serious public and life-threatening health problem ( Ehuwa et al, 2021 ; Moretta et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Dual antibacterial mechanism of [K4K15]CZS-1 against Salmonella Typhimurium: a membrane active and intracellular-targeting antimicrobial peptide

Bermúdez-Puga,

Dias,

Freire de Oliveira

et al. 2023

Front. Microbiol.

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Salmonella genus is a leading cause of food-borne infections with strong public health impact and economic ramifications. The development of antimicrobial resistance added complexity to this scenario and turned the antibiotic drug discovery into a highly important challenge. The screening of peptides has served as a successful discovery platform to design new antibiotic candidates. Motivated by this, the antimicrobial and cytotoxic properties of three cruzioseptins against Salmonella Typhimurium and RAW 264.7 murine macrophage cells, respectively, were investigated. [K4K15]CZS-1 was the most potent antimicrobial peptide identified in the screening step with a minimum inhibitory concentration (MIC) of 16 μg/mL (7.26 μM) and moderate cytotoxicity. From a structural point of view, in vitro and in silico techniques evidenced that [K4K15]CZS-1 is a α-helical cationic antimicrobial peptide. In order to capture mechanistic details and fully decipher their antibacterial action, we adopted a multidimensional approach, including spectroscopy, electron microscopy and omics analysis. In general lines, [K4K15]CZS-1 caused membrane damage, intracellular alterations in Salmonella and modulated metabolic pathways, such as the tricarboxylic acid (TCA) cycle, fatty acid biosynthesis, and lipid metabolism. Overall, these findings provide deeper insights into the antibacterial properties and multidimensional mode of action of [K4K15]CZS-1 against Salmonella Typhimurium. In summary, this study represents a first step toward the screening of membrane-acting and intracellular-targeting peptides as potential bio-preservatives to prevent foodborne outbreaks caused by Salmonella.

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